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European Journal of Applied Physiology

Erschienen in:

01.02.2013 | Original Article

Inflammatory markers in skeletal muscle of older adults

verfasst von: Marissa K. Caldow, David Cameron-Smith, Pazit Levinger, Michael J. McKenna, Itamar Levinger

Erschienen in: European Journal of Applied Physiology | Ausgabe 2/2013

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Abstract

Older adults have an increase in circulating markers of inflammation. The current study examined whether there is an increase in the expression of inflammatory markers within the vastus lateralis, a major locomotive muscle, of older adults, and if so, whether the reduction in muscle strength and aerobic capacity in older adults is related to increased muscle inflammation. Skeletal muscle biopsies were taken from older adults (n = 17, 67 ± 1.6 years) and young individuals (n = 16, 24 ± 0.6 years) under resting and fasting conditions. Muscle was analyzed for mRNA levels of intracellular inflammatory molecules (MCP1, TNFα and IL-1β) and total cellular protein abundance of cytokines, chemokines and kinases (IL-6, IL-8, MCP1, TNFα, p65 (NF-κB), JNK1/2 and STAT3). MCP1 expression was significantly higher (p < 0.05; 50 %, mRNA and 40 %, protein) in elderly than younger participants, as was IL-8 (4 %). No detectable difference in kinase protein expression was observed for STAT3, JNK or p65 (NF-κB), TNFα or IL-6. Muscle strength was lower in the elderly compared to the young group (1.55 ± 0.17 vs. 2.56 ± 0.13 Nm/kg, p < 0.001). The elderly group also had a significantly lower VO_2peak compared to the young group (24.9 ± 1.9 vs. 39.3 ± 1.9, p < 0.001), but muscle strength and VO_2peak were not correlated with the examined inflammatory markers. Older adults have increased MCP1 (mRNA and protein abundance) and IL-8 (protein abundance) and also reduced muscle strength and VO_2peak. However, the reduction in muscle strength and VO_2peak was not related to the increase in muscle inflammatory markers in this cohort.

Antonelli A, Rotondi M, Fallahi P, Ferrari SM, Paolicchi A, Romagnani P, Serio M, Ferrannini E (2006) Increase of CXC chemokine CXCL10 and CC chemokine CCL2 serum levels in normal ageing. Cytokine 34(1–2):32–38PubMedCrossRef

Baggiolini M, Dewald B, Moser B (1997) Human chemokines: an update. Ann Rev Imm 15:675–705CrossRef

Bar-Shai M, Carmeli E, Coleman R, Rozen N, Perek S, Fuchs D, Reznick AZ (2005a) The effect of hindlimb immobilization on acid phosphatase, metalloproteinases and nuclear factor-kappaB in muscles of young and old rats. Mech Ageing Dev 126(2):289–297PubMedCrossRef

Bar-Shai M, Carmeli E, Reznick AZ (2005b) The role of NF-kappaB in protein breakdown in immobilization, aging, and exercise: from basic processes to promotion of health. Ann N Y Acad Sci 1057:431–447PubMedCrossRef

Bruunsgaard H, Pedersen BK (2003) Age-related inflammatory cytokines and disease. Immunol Allergy Clin North Am 23(1):15–39PubMedCrossRef

Bruunsgaard H, Andersen-Ranberg K, Hjelmborg JB, Pedersen BK, Jeune B (2003) Elevated levels of tumor necrosis factor alpha and mortality in centenarians. Am J Med 115(4):278–283PubMedCrossRef

Buford TW, Cooke MB, Manini TM, Leeuwenburgh C, Willoughby DS (2010) Effects of age and sedentary lifestyle on skeletal muscle NF-kappaB signaling in men. J Gerontol Series A Bio Sci Med Sci 65(5):532–537CrossRef

Conboy IM, Rando TA (2005) Aging, stem cells and tissue regeneration: lessons from muscle. Cell Cycle 4(3):407–410PubMedCrossRef

Degens H (2007) Age-related skeletal muscle dysfunction: causes and mechanisms. J Musculoskelet Neuronal Interact 7(3):246–252PubMed

Deo R, Khera A, McGuire DK, Murphy SA, Meo Neto Jde P, Morrow DA, de Lemos JA (2004) Association among plasma levels of monocyte chemoattractant protein-1, traditional cardiovascular risk factors, and subclinical atherosclerosis. J Am Coll Cardiol 44(9):1812–1818

Ferrucci L, Corsi A, Lauretani F, Bandinelli S, Bartali B, Taub DD, Guralnik JM, Longo DL (2005) The origins of age-related proinflammatory state. Blood 105(6):2294–2299PubMedCrossRef

Fiatarone MA, O’Neill EF, Ryan ND, Clements KM, Solares GR, Nelson ME, Roberts SB, Kehayias JJ, Lipsitz LA, Evans WJ (1994) Exercise training and nutritional supplementation for physical frailty in very elderly people. New Engl J Med 330(25):1769–1775PubMedCrossRef

Fielding RA, Vellas B, Evans WJ, Bhasin S, Morley JE, Newman AB, Abellan van Kan G, Andrieu S, Bauer J, Breuille D, Cederholm T, Chandler J, De Meynard C, Donini L, Harris T, Kannt A, Keime Guibert F, Onder G, Papanicolaou D, Rolland Y, Rooks D, Sieber C, Souhami E, Verlaan S, Zamboni M (2011) Sarcopenia: an undiagnosed condition in older adults. Current consensus definition: prevalence, etiology, and consequences. International working group on sarcopenia. J Am Med Dir Assoc 12(4):249–256

Figarella-Branger D, Civatte M, Bartoli C, Pellissier JF (2003) Cytokines, chemokines, and cell adhesion molecules in inflammatory myopathies. Muscle Nerve 28(6):659–682PubMedCrossRef

Franceschi C, Bonafe M, Valensin S, Olivieri F, De Luca M, Ottaviani E, De Benedictis G (2000) Inflammaging an evolutionary perspective on immunosenescence. Ann N Y Acad Sci 908:244–254PubMedCrossRef

Franceschi C, Capri M, Monti D, Giunta S, Olivieri F, Sevini F, Panourgia MP, Invidia L, Celani L, Scurti M, Cevenini E, Castellani GC, Salvioli S (2007) Inflammaging and anti-inflammaging: a systemic perspective on aging and longevity emerged from studies in humans. Mech Ageing Dev 128(1):92–105PubMedCrossRef

Fulda S, Debatin KM (2002) IFNgamma sensitizes for apoptosis by upregulating caspase-8 expression through the Stat1 pathway. Oncogene 21(15):2295–2308PubMedCrossRef

Gerli R, Monti D, Bistoni O, Mazzone AM, Peri G, Cossarizza A, Di Gioacchino M, Cesarotti ME, Doni A, Mantovani A, Franceschi C, Paganelli R (2000) Chemokines, sTNF-Rs and sCD30 serum levels in healthy aged people and centenarians. Mech Ageing Dev 121(1–3):37–46PubMed

Greiwe JS, Cheng B, Rubin DC, Yarasheski KE, Semenkovich CF (2001) Resistance exercise decreases skeletal muscle tumor necrosis factor alpha in frail elderly humans. FASEB J 15(2):475–482PubMedCrossRef

Haddad F, Adams GR (2006) Aging-sensitive cellular and molecular mechanisms associated with skeletal muscle hypertrophy. J Appl Physiol 100(4):1188–1203PubMedCrossRef

Handel ML, McMorrow LB, Gravallese EM (1995) Nuclear factor-kappa B in rheumatoid synovium. Localization of p50 and p65. Arth Rheum 38(12):1762–1770

Hughes VA, Frontera WR, Roubenoff R, Evans WJ, Singh MA (2002) Longitudinal changes in body composition in older men and women: role of body weight change and physical activity. Am J Clin Nut 76(2):473–481

Inadera H, Egashira K, Takemoto M, Ouchi Y, Matsushima K (1999) Increase in circulating levels of monocyte chemoattractant protein-1 with aging. J Interf Cytokine Res 19(10):1179–1182CrossRef

Janssen I, Heymsfield SB, Wang ZM, Ross R (2000) Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr. J Appl Physiol 89(1):81–88PubMed

Kanda H, Tateya S, Tamori Y, Kotani K, Hiasa K, Kitazawa R, Kitazawa S, Miyachi H, Maeda S, Egashira K, Kasuga M (2006) MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Inv 116(6):1494–1505CrossRef

Kosek DJ, Kim JS, Petrella JK, Cross JM, Bamman MM (2006) Efficacy of 3 days/wk resistance training on myofiber hypertrophy and myogenic mechanisms in young vs. older adults. J Appl Physiol 101(2):531–544PubMedCrossRef

Krabbe KS, Pedersen M, Bruunsgaard H (2004) Inflammatory mediators in the elderly. Exp Gerontol 39(5):687–699PubMedCrossRef

Langen RC, Schols AM, Kelders MC, Van Der Velden JL, Wouters EF, Janssen-Heininger YM (2002) Tumor necrosis factor-alpha inhibits myogenesis through redox-dependent and -independent pathways. Am J Physiol Cell Physiol 283(3):C714–C721PubMed

Levinger I, Goodman C, Hare DL, Jerums G, Selig S (2007) The effect of resistance training on functional capacity and quality of life in individuals with high and low numbers of metabolic risk factors. Diabetes Care 30(9):2205–2210PubMedCrossRef

Levinger I, Levinger P, Trenerry MK, Feller JA, Bartlett JR, Bergman N, McKenna MJ, Cameron-Smith D (2011) Increased inflammatory cytokine expression in the vastus lateralis of patients with knee osteoarthritis. Arthr Rheum 63(5):1343–1348CrossRef

Liu ZG (2003) Adding facets to TNF signaling. The JNK angle. Mol Cell 12(4):795–796PubMedCrossRef

Mariani E, Cattini L, Neri S, Malavolta M, Mocchegiani E, Ravaglia G, Facchini A (2006) Simultaneous evaluation of circulating chemokine and cytokine profiles in elderly subjects by multiplex technology: relationship with zinc status. Biogerontol 7(5–6):449–459CrossRef

Miles EA, Rees D, Banerjee T, Cazzola R, Lewis S, Wood R, Oates R, Tallant A, Cestaro B, Yaqoob P, Wahle KW, Calder PC (2008) Age-related increases in circulating inflammatory markers in men are independent of BMI, blood pressure and blood lipid concentrations. Atheroscl 196(1):298–305CrossRef

Mylabathula DB, Rice KM, Wang Z, Uddemarri S, Kinnard RS, Blough ER (2006) Age-associated changes in MAPK activation in fast- and slow-twitch skeletal muscle of the F344/NNiaHSD X Brown Norway/BiNia rat model. Exp Gerontol 41(2):205–214PubMedCrossRef

Peake J, Della Gatta P, Cameron-Smith D (2010) Aging and its effects on inflammation in skeletal muscle at rest and following exercise-induced muscle injury. Am J Physiol Reg Int Comp Physiol 298(6):R1485–R1495CrossRef

Phillips T, Leeuwenburgh C (2005) Muscle fiber specific apoptosis and TNF-alpha signaling in sarcopenia are attenuated by life-long calorie restriction. FASEB J 19(6):668–670PubMed

Seidler S, Zimmermann HW, Bartneck M, Trautwein C, Tacke F (2010) Age-dependent alterations of monocyte subsets and monocyte-related chemokine pathways in healthy adults. BMC Immunol 11:30PubMedCrossRef

Shireman PK, Contreras-Shannon V, Ochoa O, Karia BP, Michalek JE, McManus LM (2007) MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leukoc Biol 81(3):775–785PubMedCrossRef

Simon AR, Rai U, Fanburg BL, Cochran BH (1998) Activation of the JAK-STAT pathway by reactive oxygen species. Am J Physiol 275(6 Pt 1):C1640–C1652PubMed

Thalacker-Mercer AE, Dell’Italia LJ, Cui X, Cross JM, Bamman MM (2010) Differential genomic responses in old vs. young humans despite similar levels of modest muscle damage after resistance loading. Physiol Genomics 40(3):141–149PubMedCrossRef

Tidball JG (2005) Inflammatory processes in muscle injury and repair. Am J Physiol Reg Int Comp Physiol 288(2):R345–R353CrossRef

Toth MJ, Matthews DE, Tracy RP, Previs MJ (2005) Age-related differences in skeletal muscle protein synthesis: relation to markers of immune activation. Am J Physiol Endocrinol Metabol 288(5):E883–E891CrossRef

Trenerry MK, Carey KA, Ward AC, Cameron-Smith D (2007) STAT3 signaling is activated in human skeletal muscle following acute resistance exercise. J Appl Physiol 102(4):1483–1489PubMedCrossRef

Trenerry MK, Carey KA, Ward AC, Farnfield MM, Cameron-Smith D (2008) Exercise-induced activation of STAT3 signaling is increased with age. Rejuvenation Res 11(4):717–724PubMedCrossRef

Trenerry MK, Della Gatta PA, Larsen AE, Garnham AP, Cameron-Smith D (2010) Impact of resistance exercise training on interleukin-6 and JAK/STAT in young men. Muscle Nerve 43(3):385–392PubMedCrossRef

Wieczorowska-Tobis K, Niemir ZI, Podkowka R, Korybalska K, Mossakowska M, Breborowicz A (2006) Can an increased level of circulating IL-8 be a predictor of human longevity? Med Sci Monit 12(3):CR118–CR121PubMed

Williamson D, Gallagher P, Harber M, Hollon C, Trappe S (2003) Mitogen-activated protein kinase (MAPK) pathway activation: effects of age and acute exercise on human skeletal muscle. J Physiol 547(Pt 3):977–987PubMedCrossRef

Titel: Inflammatory markers in skeletal muscle of older adults
verfasst von: Marissa K. Caldow
David Cameron-Smith
Pazit Levinger
Michael J. McKenna
Itamar Levinger
Publikationsdatum: 01.02.2013
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 2/2013
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-012-2458-x

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