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01.09.2014 | Original Article

Irisin and FNDC5: effects of 12-week strength training, and relations to muscle phenotype and body mass composition in untrained women

verfasst von: S. Ellefsen, O. Vikmoen, G. Slettaløkken, J. E. Whist, H. Nygaard, I. Hollan, I. Rauk, G. Vegge, T. A. Strand, T. Raastad, B. R. Rønnestad

Erschienen in: European Journal of Applied Physiology | Ausgabe 9/2014

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Abstract

Purpose

To investigate the effects of strength training on abundances of irisin-related biomarkers in skeletal muscle and blood of untrained young women, and their associations with body mass composition, muscle phenotype and levels of thyroid hormones.

Methods

Eighteen untrained women performed 12 weeks of progressive whole-body heavy strength training, with measurement of strength, body composition, expression of irisin-related genes (FNDC5 and PGC1α) in two different skeletal muscles, and levels of serum-irisin and -thyroid hormones, before and after the training intervention.

Results

The strength training intervention did not result in changes in serum-irisin or muscle FNDC5 expression, despite considerable effects on strength, lean body mass (LBM) and skeletal muscle phenotype. Our data indicate that training affects irisin biology in a LBM-dependent manner. However, no association was found between steady-state serum-irisin or training-associated changes in serum-irisin and alterations in body composition. FNDC5 expression was higher in m.Biceps brachii than in m.Vastus lateralis, with individual expression levels being closely correlated, suggesting a systemic mode of transcriptional regulation. In pre-biopsies, FNDC5 expression was correlated with proportions of aerobic muscle fibers, a relationship that disappeared in post-biopsies. No association was found between serum-thyroid hormones and FNDC5 expression or serum-irisin.

Conclusion

No evidence was found for an effect of strength training on irisin biology in untrained women, though indications were found for a complex interrelationship between irisin, body mass composition and muscle phenotype. FNDC5 expression was closely associated with muscle fiber composition in untrained muscle.

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Throughout the manuscript, the term “steady-state” refers to gene expression/hormone abundance measured in biological material sampled at rest (in this study: >48 h since last training session).

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Titel: Irisin and FNDC5: effects of 12-week strength training, and relations to muscle phenotype and body mass composition in untrained women
verfasst von: S. Ellefsen
O. Vikmoen
G. Slettaløkken
J. E. Whist
H. Nygaard
I. Hollan
I. Rauk
G. Vegge
T. A. Strand
T. Raastad
B. R. Rønnestad
Publikationsdatum: 01.09.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Applied Physiology / Ausgabe 9/2014
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-014-2922-x

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Abstract

Purpose

Methods

Results

Conclusion

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