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01.01.2014 | Original Article

Physical exercise increases GFAP expression and induces morphological changes in hippocampal astrocytes

verfasst von: Lisiani Saur, Pedro Porto Alegre Baptista, Priscylla Nunes de Senna, Mariana Fontoura Paim, Patricia do Nascimento, Jocemar Ilha, Pamela Brambilla Bagatini, Matilde Achaval, Léder Leal Xavier

Erschienen in: Brain Structure and Function | Ausgabe 1/2014

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Abstract

Physical exercise has an important influence on brain plasticity, which affects the neuron–glia interaction. Astrocytes are susceptible to plasticity, and induce and stabilize synapses, regulate the concentration of various molecules, and support neuronal energy metabolism. The aim of our study was to investigate whether physical exercise is capable of altering the morphology, density and expression of glial fibrillary acidic protein (GFAP) in astrocytes from the CA1 region of rat hippocampus. Thirteen male rats were divided in two groups: sedentary (n = 6) and exercise (n = 7). The animals in the exercise group were submitted to a protocol of daily physical exercise on a treadmill for four consecutive weeks. GFAP immunoreactivity was evaluated using optical densitometry and the morphological analyses were an adaptation of Sholl’s concentric circles method. Our results show that physical exercise is capable of increasing the density of GFAP-positive astrocytes as well as the regional and cellular GFAP expression. In addition, physical exercise altered astrocytic morphology as shown by the increase observed in the degree of ramification in the lateral quadrants and in the length of the longest astrocytic processes in the central quadrants. Our data demonstrate important changes in astrocytes promoted by physical exercise, supporting the idea that these cells are involved in regulating neural activity and plasticity.

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Titel: Physical exercise increases GFAP expression and induces morphological changes in hippocampal astrocytes
verfasst von: Lisiani Saur
Pedro Porto Alegre Baptista
Priscylla Nunes de Senna
Mariana Fontoura Paim
Patricia do Nascimento
Jocemar Ilha
Pamela Brambilla Bagatini
Matilde Achaval
Léder Leal Xavier
Publikationsdatum: 01.01.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Brain Structure and Function / Ausgabe 1/2014
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-012-0500-8

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