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01.04.2012 | Original Article—Alimentary Tract

Endogenous orexin-A in the brain mediates 2-deoxy-d-glucose-induced stimulation of gastric motility in freely moving conscious rats

verfasst von: Tsukasa Nozu, Yoshihiro Tuchiya, Shima Kumei, Kaoru Takakusaki, Koji Ataka, Mineko Fujimiya, Toshikatsu Okumura

Erschienen in: Journal of Gastroenterology | Ausgabe 4/2012

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Abstract

Background

Increasing evidence has indicated that brain orexin plays a vital role in the regulation of gastrointestinal (GI) physiology such as gastric acid secretion and GI motility. The aim of this study was to elucidate the effects and mechanisms of orexin on gastric motility in non-fasted rats.

Methods

In this study, we recorded intraluminal gastric pressure waves in freely moving conscious rats with a manometric catheter located in the antrum. We assessed the area under the manometric trace as the motor index (MI), and compared its values for 1 h before and after drug administration.

Results

Intracisternal (ic) injection of orexin-A (10 μg) significantly increased the MI, but intraperitoneal (ip) injection did not have any effect. Pretreatment of ip injection of atropine significantly blocked the orexin-A-induced stimulation of gastric motility. Intravenous injection of 2-deoxy-d-glucose (2-DG, 200 mg/kg), a central vagal stimulant, significantly increased the MI. The ic injection of SB-334687 (40 μg), a selective orexin-A antagonist, did not modify the basal MI, but this antagonist significantly suppressed the stimulant action of 2-DG.

Conclusions

These results suggest that endogenous orexin-A in the brain is involved in the vagal-dependent stimulation of gastric contractions.

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Titel: Endogenous orexin-A in the brain mediates 2-deoxy-d-glucose-induced stimulation of gastric motility in freely moving conscious rats
verfasst von: Tsukasa Nozu
Yoshihiro Tuchiya
Shima Kumei
Kaoru Takakusaki
Koji Ataka
Mineko Fujimiya
Toshikatsu Okumura
Publikationsdatum: 01.04.2012
Verlag: Springer Japan
Erschienen in: Journal of Gastroenterology / Ausgabe 4/2012
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI: https://doi.org/10.1007/s00535-011-0506-7

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Endogenous orexin-A in the brain mediates 2-deoxy-d-glucose-induced stimulation of gastric motility in freely moving conscious rats