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01.09.2013 | Original Article—Alimentary Tract

Overexpressed miR-301a promotes cell proliferation and invasion by targeting RUNX3 in gastric cancer

verfasst von: Ming Wang, Chenglong Li, Beiqin Yu, Liping Su, Jianfang Li, Jingfang Ju, Yingyan Yu, Qinlong Gu, Zhenggang Zhu, Bingya Liu

Erschienen in: Journal of Gastroenterology | Ausgabe 9/2013

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Abstract

Background

MicroRNAs can promote or suppress the evolution of malignant behaviors by regulating multiple targets. We aimed to determine the expression of miR-301a recently screened in gastric cancer, to investigate the biological effects of miR-301a and to identify the specific miR-301a target gene.

Methods

Quantitative real-time RT-PCR was used to test miR-301a expression. Functional effects were explored by a water-soluble tetrazolium salt assay, a colony formation assay in soft agar, a migration assay, an invasion assay and cytometry used to determine apoptosis and cell cycle. Nude mice were inoculated subcutaneously by retrovirus-mediated stably expressed SGC-7901 cells. The target gene was determined by bioinformatic algorithms, dual luciferase reporter assay and Western blot.

Results

Firstly, we found that miR-301a was significantly upregulated both in cells and tissues of gastric cancer. The expression level of miR-301a was inversely correlated with tumor differentiation of gastric cancer tissues. Secondly, miR-301a promoted cell growth, soft agar clonogenicity, migration, invasion, and decreased cell apoptosis induced by cisplatin in vitro, while blockage of miR-301a reduced the percentage of G2/M phase cells via flow cytometry in gastric cancer cells. Ectopic expression of miR-301a enhanced the subcutaneous tumorigenesis in vivo. Finally, miR-301a directly downregulated RUNX3 expression post-transcriptionally in gastric cancer.

Conclusion

Our results demonstrate that miR-301a plays important roles in the development of gastric cancer.

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Titel: Overexpressed miR-301a promotes cell proliferation and invasion by targeting RUNX3 in gastric cancer
verfasst von: Ming Wang
Chenglong Li
Beiqin Yu
Liping Su
Jianfang Li
Jingfang Ju
Yingyan Yu
Qinlong Gu
Zhenggang Zhu
Bingya Liu
Publikationsdatum: 01.09.2013
Verlag: Springer Japan
Erschienen in: Journal of Gastroenterology / Ausgabe 9/2013
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI: https://doi.org/10.1007/s00535-012-0733-6

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Abstract

Background

Methods

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Conclusion

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