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01.11.2015 | Original Article

Genetic evaluation of bone marrow-derived mesenchymal stem cells by a modified karyotyping method

verfasst von: Seyedeh-Leili Asadi-Yousefabad, Azizollah Khodakaram-Tafti, Mehdi Dianatpour, Davood Mehrabani, Shahrokh Zare, Amin Tamadon, Saman Nikeghbalian, Alireza Raayat-Jahromi, Somayeh Ahmadlou

Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2015

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Abstract

Bone marrow is the most important source of mesenchymal stem cells (MSCs). Significant development in the comprehension of MSCs biology has opened the way to their clinical use. The object of this study was to establish a method for karyotyping of canine MSCs. We examined the effect of the increase in passage number on the chromosome and appearance of MSCs. A large 3-year-old mixed breed dog was used. Bone marrow (20 ml) was collected from the proximal humerus under general anesthesia and cultured and passaged in alpha minimal essential medium (αMEM) and fetal bovine serum (FBS). The canine bone marrow MSCs (BM-MSCs) were characterized by reverse transcription polymerase chain reaction (RT-PCR) and flask adherent fibroblast-like morphology. Karyotyping of cells of passages 1 and 8 was performed. The chromosome numbers were counted and evaluated to obtain the percentage of diploid cells. The best time for exposing BM-MSCs with media containing colcemid was 10 h. These cells were positive for the BM-MSCs markers CD90 and negative for hematopoietic markers CD34 and CD45 by RT-PCR. Karyotyping of these cells was normal, and also fibroblast-like cells were observed in every passage.

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Titel: Genetic evaluation of bone marrow-derived mesenchymal stem cells by a modified karyotyping method
verfasst von: Seyedeh-Leili Asadi-Yousefabad
Azizollah Khodakaram-Tafti
Mehdi Dianatpour
Davood Mehrabani
Shahrokh Zare
Amin Tamadon
Saman Nikeghbalian
Alireza Raayat-Jahromi
Somayeh Ahmadlou
Publikationsdatum: 01.11.2015
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 6/2015
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-015-2081-4

Springer Medizin

Genetic evaluation of bone marrow-derived mesenchymal stem cells by a modified karyotyping method

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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