nach oben

Erschienen in:

16.02.2018 | Original Article

Molecular detection of Leishmania species in northeast of Iran

verfasst von: Mohammad Javad Namazi, Azar Balooti Dehkordi, Faezeh Haghighi, Mohammad Mohammadzadeh, Mehdi Zarean, Morteza Haghighi Hasanabad

Erschienen in: Comparative Clinical Pathology | Ausgabe 3/2018

Einloggen, um Zugang zu erhalten

Abstract

Two known types of cutaneous leishmaniasis (CL) including zoonotic CL due to Leishmania major and anthroponotic CL due to Leishmania tropica are prevalent in 14 of 22 countries located in the Eastern Mediterranean region including Iran. According to existing data, CL is endemic in Sabzevar City (northeast of Iran) and, because of the climatic conditions in this semi-desert region, is suitable for living vector/reservoir hosts of infection. The aim of our study was to identify the recent status of CL causative species in rural areas of Sabzevar County. Suspected patients of CL who were referred to health centers in suburban areas of Sabzevar and confirmed via microscopic observation of amastigotes were included in the study. Molecular identification of Leishmania species was done via nested PCR assay, based on amplification of kinetoplast minicircle fragments of L. major and L. tropica. In total, 153 patients including 89 males and 64 females were enrolled in this study. A high infection rate was reported in the autumn season (with a peak in October). Our findings revealed that L. major is responsible for 100% of infections. In addition, there was no association between CL and risk factors after statistical analysis. It seems that the infection pattern of CL is changing predominantly to L. major in most regions of Iran, which may be due to environmental changes, or ecological amendment and their effects on (vector/reservoir) host distribution in rural parts. Finally, controlling programs as well as promotion in public health systems should be considered in this area.

Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, Boer M, the WHO Leishmaniasis Control Team (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7(5):e35671CrossRefPubMedPubMedCentral

Arjmand R, Saberi S, Tolouei S, Chizari Z, Nobari RF, Fard SS et al (2014) Identification of Leishmania isolates from Varzaneh city, Isfahan province, Iran using nested polymerase chain reaction method. Adv Biomed Res 3:167CrossRefPubMedPubMedCentral

Asgari Nezhad H, Mirzaie M, Sharifi I, Zarean M, Norouzi M (2012) The prevalence of cutaneous leishmaniasis in school children in southwestern Iran, 2009. Comp Clin Pathol 21(5):1065–1069CrossRef

Azizi K, Badzohreh A, Sarkari B, Fakoorziba MR, Kalantari M, Moemenbellah-Fard MD, Ali-Akbarpour M (2013) Nested polymerase chain reaction and sequence-based detection of leishmania infection of sand flies in recently emerged endemic focus of zoonotic cutaneous leishmaniasis, southern Iran. Iran J Med Sci 38(2 Suppl):156–162PubMedPubMedCentral

Doroodgar A, Sayyah M, Doroodgar M, Mahbobi S, Nemetian M, Rafizadeh S, Rassi Y (2012) Progressive increasing of cutaneous leishmaniasis in Kashan District, central of Iran. Asian Pac J Trop Dis 2(4):260–263CrossRef

Farahmand M, Nahrevanian H, Shirazi HA, Naeimi S, Farzanehnejad Z (2011) An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran. Braz J Infect Dis 15(1):17–21CrossRefPubMed

Feiz Haddad MH, Ghasemi E, Maraghi S, Tavala M (2016) Identification of Leishmania species isolated from human cutaneous leishmaniasis in Mehran, western Iran using nested PCR. Iran J Parasitol 11(1):65–72PubMedPubMedCentral

Gillespie PM, Beaumier CM, Strych U, Hayward T, Hotez PJ, Bottazzi ME (2016) Status of vaccine research and development of vaccines for leishmaniasis. Vaccine 34(26):2992–2995CrossRefPubMed

Hamzavi Y, Khademi N (2015) Trend of cutaneous leishmaniasis in Kermanshah province, west of Iran from 1990 to 2012. Iran J Parasitol 10(1):78–86PubMedPubMedCentral

Hosseininasab A, Sharifi I, Mohammad Hossein D, Zarean M, Dadkhah M (2014) Causes of pediatric visceral leishmaniasis in southeastern Iran. Iran J Parasitol 9(4):584–587PubMedPubMedCentral

Izadi S, Mirhendi H, Jalalizand N, Khodadadi H, Mohebali M, Nekoeian S, Jamshidi A, Ghatee MA (2016) Molecular epidemiological survey of cutaneous leishmaniasis in two highly endemic metropolises of Iran, application of FTA cards for DNA extraction from Giemsa-stained slides. Jundishapur J Microbiol 9(2):e32885CrossRefPubMedPubMedCentral

Kassiri H, Sharifinia N, Jalilian M, Shemshad K (2012) Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007). Asian Pac J Trop Dis 2:S382–S3S6CrossRef

Kassiri H, Kassiri A, Lotfi M, Farajifard P, Kassiri E (2014) Laboratory diagnosis, clinical manifestations, epidemiological situation and public health importance of cutaneous leishmaniasis in Shushtar County, southwestern Iran. J Acute Dis 3(2):93–98CrossRef

Kavarizadeh F, Khademvatan S, Vazirianzadeh B, Feizhaddad MH, Zarean M (2017) Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran. J Parasit Dis 41(1):274–281CrossRefPubMed

Kermanjani A, Akhlaghi L, Oormazdi H, Hadighi R (2016) Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran. J Parasitic Dis 1–5

Khosravi A, Sharifi I, Dortaj E, Aghaei Afshar A, Mostafavi M (2013) The present status of cutaneous leishmaniasis in a recently emerged focus in south-west of Kerman Province, Iran. Iran J Public Health 42(2):182–187PubMedPubMedCentral

Mahmoudvand H, Mohebali M, Sharifi I, Keshavarz H, Hajjaran H, Akhoundi B, Jahanbakhsh S, Zarean M, Javadi A (2011) Epidemiological aspects of visceral leishmaniasis in Baft district, Kerman Province, southeast of Iran. Iran J Parasitol 6(1):1–11PubMedPubMedCentral

Maraghi S, Mardanshah O, Rafiei A, Samarbafzadeh A, Vazirianzadeh B (2013) Identification of cutaneous leishmaniasis agents in four geographical regions of Khuzestan Province using nested PCR. Jundishapur J Microbiol 6(4)

Mathers CD, Ezzati M, Lopez AD (2007) Measuring the burden of neglected L. tropica diseases: the global burden of disease framework. PLoS Negl Trop Dis 1(2):e114CrossRefPubMedPubMedCentral

Mesgarian F, Rahbarian N, Mahmoudi Rad M, Hajaran H, Shahbaz F, Mesgarian Z et al (2010) Identification of Leishmania species isolated from human cutaneous Leishmaniasis in Gonbad-e-Qabus city using a PCR method during 2006–2007. Tehran Univ Med J 68(4):250–256

Mirahmadi H, Salimi Khorashad A, Sohrabnahad A, Heydarian P, Bizhani N (2016) Species identification and molecular typing of Leishmania spp. using targeting HSP70 gene in suspected patients of cutaneous Leishmaniasis from Sistan and Baluchestan Province, Southeast Iran. Iran J Parasitol 11(4):489–498PubMedPubMedCentral

Mohajery M, Shamsian SA, Rezaee AR, Hassanpoor K, Shakeri MT, Farnoosh GH (2010) Evaluation of molecular epidemiology of cutaneous leishmaniasis in Sabzevar. J Mashhad Univ Med Sci 53:138–144

Mohammadi F, Narimani M, Nekoian S, Shirani Bidabadi L, Mohammadi F, Hosseini M et al (2011) Identification and isolation of the cause of cutaneous leishmaniasis in Is-fahan using ITS-PCR. J Isfahan Med Sch 30:1–7

Murray CJ, Barber RM, Foreman KJ, Abbasoglu Ozgoren A, Abd-Allah F, Abera SF et al (2015) Global, regional, and national disability-adjusted life years (DALYs) for 306 diseases and injuries and healthy life expectancy (HALE) for 188 countries, 1990–2013: quantifying the epidemiological transition. Lancet 386(10009):2145–2191CrossRefPubMed

Nateghi Rostami M, Saghafipour A, Vesali E (2013) A newly emerged cutaneous leishmaniasis focus in central Iran. Int J Infect Dis 17(12):e1198–e1206CrossRefPubMed

Noyes HA, Reyburn H, Bailey JW, Smith D (1998) A nested-PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan. J Clin Microbiol 36(10):2877–2881PubMedPubMedCentral

Postigo J (2016) Leishmaniasis in high-burden countries: an epidemiological update based on data reported in 2016. Weekly epidemiological record (http://who.int/wer/2016/wer9122)

Pour R, Sharifi I, Kazemi B, Zarean M (2011) Identification of nonresponsive isolates to glucantime in patients with cutaneous leishmanaisis in Bam. J Kerman Univ Med Sci 18(2):123–133

Pouresmaeliyan S, Mirzaei M, Sharifi I, Zarean M (2011) The prevalence of cutaneous leishmaniasis in the city and suburb of Mohammadabad, Jiroft District and identification of parasite species by nested-PCR, 2008. J Kerman Univ Med Sci 18(3):218–227

Schwarz NG, Loderstaedt U, Hahn A, Hinz R, Zautner AE, Eibach D et al (2015) Microbiological laboratory diagnostics of neglected zoonotic diseases (NZDs). Acta Trop 15(7):75–78

Sharifi F, Sharifi I, Zarean M, Hakimi Parizi M, Aflatoonian MR, Fasihi Harandi M et al (2012) Spatial distribution and molecular identification of Leishmania species from endemic foci of south-eastern Iran. Iran J Parasitol 7(1):45–52PubMedPubMedCentral

Shiee MR, Hajjaran H, Mohebali M, Doroodgar A, Saadat MH, Teimouri A et al (2012) A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran. Asian Pac J Trop Dis 2(6):421–425CrossRef

Shirian S, Oryan A, Hatam GR, Panahi S, Daneshbod Y (2014) Comparison of conventional, molecular, and immunohistochemical methods in diagnosis of typical and atypical cutaneous leishmaniasis. Arch Pathol Lab Med 138(2):235–240CrossRefPubMed

Shlomai J (2004) The structure and replication of kinetoplast DNA. Curr Mol Med 4:623–647CrossRefPubMed

Tolouei S, Hejazi SH, Ghaedi K, Hasheminia SJ (2014) Identification of leishmania isolates from healing and nonhealing cutaneous leishmaniasis patients using internal transcribed spacer region PCR. Jundishapur J Microbiol 7(4):e9529CrossRefPubMedPubMedCentral

Vazirianzadeh B, Saki J, Jahanifard E, Zarean M, Amraee K, Navid Pour S (2013) Isolation and identification of Leishmania species from sandflies and rodents collected from Roffaye District, Khuzestan Province, southwest of Iran. Jundishapur J Microbiol 6(6):e10025CrossRef

Zarean M, Maraghi S, Hajjaran H, Mohebali M, Feiz-Hadad MH, Assarehzadegan MA (2015) Comparison of proteome profiling of two sensitive and resistant field Iranian isolates of Leishmania major to Glucantime® by 2-dimensional electrophoresis. Iran J Parasitol 10(1):19–29PubMedPubMedCentral

Zarean M, Maraghi SH, Hajjaran H, Mohebali M, Feiz-Haddad MH, Assarehzadegan MA (2017) Correlation between clinical responses with the drug-susceptibility of parasites in Iranian cutaneous leishmaniasis caused by Leishmania major. Trop Biomed 34(2):338–345

Titel: Molecular detection of Leishmania species in northeast of Iran
verfasst von: Mohammad Javad Namazi
Azar Balooti Dehkordi
Faezeh Haghighi
Mohammad Mohammadzadeh
Mehdi Zarean
Morteza Haghighi Hasanabad
Publikationsdatum: 16.02.2018
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 3/2018
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2658-9

Springer Medizin

Molecular detection of Leishmania species in northeast of Iran

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2018

Potential pharmaceutic effect of Shilajit (mumie) on experimental osteoarthritis in rat

Is vitamin D supplement accompanied with narrow band UVB effective for treatment of vitiligo?

Outbreak and management of Mycoplasma gallisepticum infection in desi chicken and turkey flocks in an organized mixed farm

Potential of the ImageJ software in the differentiation of various types of canine lymphadenopathies with fine needle aspiration cytology (FNAC)

Hematological and plasm biochemical values for Rhinogobio ventralis in the Yangtze River, China

Chronic immune thrombocytopenia. Egyptian experience

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie