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01.06.2013 | Original Article

Regulatory T cells in the blood: a new marker of surgical stress

verfasst von: Yu Saito, Mitsuo Shimada, Tohru Utsunomiya, Yuji Morine, Satoru Imura, Tetsuya Ikemoto, Hiroki Mori, Jun Hanaoka, Shuichi Iwahashi, Shinichiro Yamada, Michihito Asanoma

Erschienen in: Surgery Today | Ausgabe 6/2013

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Abstract

Purpose

The function of regulatory T cells (Foxp3⁺CD4⁺CD25⁺ T cells: T_reg) after surgery remains unspecified. We investigated the potential role of T_reg as a new stress marker for various operations.

Methods

Thirty-three patients who underwent various operations at our department were divided into the following three groups based on the invasiveness of their surgery: Group A, those who underwent massively invasive surgery; Group B, those who underwent moderately invasive surgery; and Group C, those who underwent minimally invasive surgery. We measured T_reg levels in the peripheral blood by flow cytometry and labeling with anti-CD4, CD25, and Foxp3 antibodies on preoperative day 1 and then on postoperative days (PODs) 1 and 6. T_reg subpopulations in each group on the 3 days were compared.

Results

T_reg subpopulations were significantly higher on POD 6 than on preoperative day 1 in all patients. In Group B, T_reg subpopulations varied according to the operative procedures undertaken. For example, there were marked differences between open and laparoscopic abdominal surgery. In Group A, the T_reg subpopulations tended to be increased on POD 6, although those on POD 1 were lower than those on preoperative day 1.

Conclusions

These findings suggest that T_reg is an efficient biomarker, indicative of the degree of surgical stress and its impact on immunological status.

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Titel: Regulatory T cells in the blood: a new marker of surgical stress
verfasst von: Yu Saito
Mitsuo Shimada
Tohru Utsunomiya
Yuji Morine
Satoru Imura
Tetsuya Ikemoto
Hiroki Mori
Jun Hanaoka
Shuichi Iwahashi
Shinichiro Yamada
Michihito Asanoma
Publikationsdatum: 01.06.2013
Verlag: Springer Japan
Erschienen in: Surgery Today / Ausgabe 6/2013
Print ISSN: 0941-1291
Elektronische ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-013-0517-5

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Abstract

Purpose

Methods

Results

Conclusions

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