Erschienen in:
01.09.2012 | Brief Report
First report of bovine herpesvirus 5 in bull semen
verfasst von:
Majela Rodríguez, Maritza Barrera, Oliberto Sánchez, Eya Caridad Rodríguez, Nadia Martínez, Natalie C. Parra, Jorge R. Toledo
Erschienen in:
Archives of Virology
|
Ausgabe 9/2012
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Abstract
BoHV-5 was detected in one of several extended semen samples from a healthy donor bull during routine virus screening. This was achieved by polymerase chain reaction assay (PCR) and virus isolation, with primary identification by the fluorescent antibody test. The isolated virus, B4180, was characterized by sequencing a cloned fragment of the gC gene and by restriction enzyme analysis (REA). The nucleotide sequence shared 99 % similarity with published sequences of BoHV-5, and the REA showed that the isolate was of the BoHV-5a subtype. This study provides the first evidence of intermittent BoHV-5 shedding in bull semen as well as information about its geographic distribution.