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Archives of Virology

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26.12.2016 | Original Article

Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants

verfasst von: Rachelle Bester, Johan T. Burger, Hans J. Maree

Erschienen in: Archives of Virology | Ausgabe 4/2017

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Abstract

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs (sRNA) that play an essential role in the regulation of target mRNAs expressed during plant development and in response to stress. MicroRNA expression profiling has helped to identify miRNAs that regulate a range of processes, including the plant’s defence response to pathogens. In this study, differential miRNA expression in own-rooted Vitis vinifera cv. Cabernet Sauvignon plants infected with grapevine leafroll-associated virus 3 was investigated with microarrays and next-generation sequencing (NGS) of sRNA and mRNA. These high-throughput approaches identified several differentially expressed miRNAs. Four miRNAs, identified by both approaches, were validated by stemloop RT-PCRs. Three of the predicted targets of the differentially expressed miRNAs were also differentially expressed in the transcriptome data of infected plants, and were validated by RT-qPCR. Identification of these miRNAs and their targets can lead to a better understanding of host-pathogen interactions involved in grapevine leafroll disease and the identification of possible targets for virus resistance.

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Titel: Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants
verfasst von: Rachelle Bester
Johan T. Burger
Hans J. Maree
Publikationsdatum: 26.12.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-3197-9

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