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Clinical Oral Investigations

Erschienen in:

23.12.2015 | Original Article

Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft

verfasst von: Gülnihal Eren, Alpdoğan Kantarcı, Anton Sculean, Gül Atilla

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2016

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Abstract

Objective

The aim of this study was to evaluate histologically the following treatment of bilateral localized gingival recessions with coronally advanced flap (CAF) combined with platelet-rich fibrin (PRF) or subepithelial connective tissue graft (SCTG).

Materials and methods

Tissue samples were harvested from 14 subjects either 1 or 6 months after the surgeries. The 2-mm punch biopsies were obtained from the mid-portion of the grafted sites. Neutral buffered formalin fixed, paraffin-embedded 5-μm thick tissue sections were stained with hematoxylin eosin and Masson’s trichrome in order to analyze the collagen framework, epithelium thickness and rete-peg length. Multiple sequential sections were cut from paraffin-embedded blocks of tissue and immunohistochemically prepared for detection of vascular endothelial growth factor, CD31 and CD34, for the assessment of vascularization.

Results

Rete peg formation was significantly increased in the sites treated with PRF compared to the SCTG group after 6 months (p < 0.05). On the contrary, the number of vessels was increased in the SCTG group compared to the PRF group after 6 months (p < 0.05). No statistically significant differences were observed in the collagen density. Staining intensity of CD31 increased in submucosal area of PRF group than SCTG group after 1 month. Higher staining intensity of CD34 was observed in the submucosal area of PRF group compared with SCTG group after 6 months.

Conclusions

The results of the present study suggest that in histological evaluation because of its biological compounds, PRF results earlier vessel formation and tissue maturation compared to connective tissue graft.

Clinical relevance

PRF regulated the vascular response associated with an earlier wound healing.

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Titel: Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft
verfasst von: Gülnihal Eren
Alpdoğan Kantarcı
Anton Sculean
Gül Atilla
Publikationsdatum: 23.12.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1697-8

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Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

Neu im Fachgebiet Zahnmedizin

Endlich: Zi zeigt, mit welchen PVS Praxen zufrieden sind

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Newsletter

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

Objective

Materials and methods

Results

Conclusions

Clinical relevance

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