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Lasers in Medical Science
Erschienen in: Lasers in Medical Science 1/2018

30.09.2017 | Original Article

Influence of pre-irradiation time employed in antimicrobial photodynamic therapy with diode laser

verfasst von: Ana Caroline Fumes, Priscilla Coutinho Romualdo, Rachel Maciel Monteiro, Evandro Watanabe, Silmara Aparecida Milori Corona, Maria Cristina Borsatto

Erschienen in: Lasers in Medical Science | Ausgabe 1/2018

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Abstract

The aim of the present study was to evaluate, in vitro, the effect of different pre-irradiation times of the photosensitizer in photodynamic therapy in biofilms formed by Streptococcus mutans and Candida albicans, through the evaluation of the microbial load. The factors under study were as follows: times of pre-irradiation of the photosensitizer in three levels (1, 2, or 5 min). For the control of the cariogenic dental biofilm with antimicrobial photodynamic therapy (aPDT), methylene blue (0.01%) was used in association with the diode laser (InGaAlP) with a wavelength of 660 nm. Chlorhexidine digluconate (0.12% CHX) and saline were used as positive and negative controls, respectively. The study design was carried out in complete and randomized blocks. The sample consisted of 15 S. mutans biofilms cultures, randomly divided into five groups and 15 C. albicans cultures, also divided into five groups. The experiment was performed in triplicate (n = 3) and the response variables were obtained through quantitative analysis of bacterial viability, expressed in colony-forming units (CFU) per square millimeter of the specimen area. The data were analyzed with the aid of the ANOVA one-way test and Tukey’s post-test. All analyses were performed using the Graph Pad Prism 4.0 program, with a significance level of 5%. For the S. mutans group, only the saline solution presented a statistically significant difference when compared to the other treatments (p < 0.05), that is, the treatment with aPDT, irrespective of the irradiation time applied, was similar to the treatment with CHX and both were more effective in reducing cariogenic biofilm compared to saline. For the group of C. albicans, there was no statistical difference between the groups (p > 0.05). Therefore, it can be concluded that the treatment with aPDT reduced the number of CFUs of S. mutans in a similar way to CHX, independently of the pre-irradiation time applied. No effect of this therapy or of the different pre-irradiation times on the C. albicans biofilm could be observed. In this way, the pre-irradiation time of 1 min can be used to reduce the microbial load of S. mutans.
Literatur
1.
Iwano Y, Sugano N, Matsumoto K, Nishihara R, Iizuka T, Yoshinuma N et al (2010) Salivary microbial levels in relation to periodontal status and caries development. J Periodontal Res 45(2):165–169CrossRefPubMed
2.
Nelson-Filho P, Olmedo LY, Andrucioli MC, Saraiva Mda C, Matsumoto MA, de Queiroz AM et al (2011) Use of the checkerboard DNA-DNA hybridisation technique for in vivo detection of cariogenic microorganisms on metallic brackets, with or without use of an antimicrobial agent. J Dent 39(7):513–517CrossRefPubMed
3.
Saravia ME, Nelson-Filho P, Silva RA, De Rossi A, Faria G, Silva LA et al (2013) Recovery of mutans streptococci on MSB, SB-20 and SB-20M agar media. Arch Oral Biol 58(3):311–316CrossRefPubMed
4.
Metwalli KH, Khan SA, Krom BP, Jabra-Rizk MA (2013) Streptococcus mutans, Candida albicans, and the human mouth: a sticky situation. PLoS Pathog 9(10):e1003616CrossRefPubMedPubMedCentral
5.
Falsetta ML, Klein MI, Colonne PM, Scott-Anne K, Gregoire S, Pai CH et al (2014) Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo. Infect Immun 82(5):1968–1981CrossRefPubMedPubMedCentral
6.
7.
Samaranayake LP, MacFarlane TW (1981) A retrospective study of patients with recurrent chronic atrophic candidosis. Oral Surg Oral Med Oral Pathol 52(2):150–153CrossRefPubMed
8.
9.
Allen CM (1994) Animal models of oral candidiasis. A review. Oral Surg Oral Med Oral Pathol 78(2):216–221CrossRefPubMed
10.
Ciancio SG (1989) Adjunctive uses of topical antimicrobial mouthrinses. Am J Dent 2:299–302PubMed
11.
Addy M, Wade W, Goodfield S (1991) Staining and antimicrobial properties in vitro of some chlorhexidine formulations. Clin Prev Dent 13(1):13–17
12.
Soukos NS, Goodson JM (2011) Photodynamic therapy in the control of oral biofilms. Periodontol 55(1):143–166CrossRef
13.
Zanin IC, Goncalves RB, Junior AB, Hope CK, Pratten J (2005) Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 56(2):324–330CrossRefPubMed
14.
Zanin IC, Lobo MM, Rodrigues LK, Pimenta LA, Hofling JF, Goncalves RB (2006) Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur J Oral Sci 114(1):64–69CrossRefPubMed
15.
Wood S, Metcalf D, Devine D, Robinson C (2006) Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother 57(4):680–684CrossRefPubMed
16.
Basso FG, Oliveira CF, Fontana A, Kurachi C, Bagnato VS, Spolidorio DM et al (2011) In vitro effect of low-level laser therapy on typical oral microbial biofilms. Braz Dent J 22(6):502–510CrossRefPubMed
17.
Mang TS, Tayal DP, Baier R (2012) Photodynamic therapy as an alternative treatment for disinfection of bacteria in oral biofilms. Lasers Surg Med 44(7):588–596CrossRefPubMed
18.
Wilson M, Burns T, Pratten J, Pearson GJ (1995) Bacteria in supragingival plaque samples can be killed by low-power laser light in the presence of a photosensitizer. J Appl Bacteriol 78(5):569–574CrossRefPubMed
19.
Wood S, Nattress B, Kirkham J, Shore R, Brookes S, Griffiths J et al (1999) An in vitro study of the use of photodynamic therapy for the treatment of natural oral plaque biofilms formed in vivo. J Photochem Photobiol B 50(1):1–7CrossRefPubMed
20.
Lima JP, Sampaio de Melo MA, Borges FM, Teixeira AH, Steiner-Oliveira C, Nobre Dos Santos M et al (2009) Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries. Eur J Oral Sci 117(5):568–574CrossRefPubMed
21.
Guglielmi Cde A, Simionato MR, Ramalho KM, Imparato JC, Pinheiro SL, Luz MA (2011) Clinical use of photodynamic antimicrobial chemotherapy for the treatment of deep carious lesions. J Biomed Opt 16(8):088003CrossRefPubMed
22.
O’Neill JF, Hope CK, Wilson M (2002) Oral bacteria in multi-species biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg Med 31(2):86–90CrossRefPubMed
23.
Muller P, Guggenheim B, Schmidlin PR (2007) Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 115(1):77–80CrossRefPubMed
24.
Pereira CA, Romeiro RL, Costa AC, Machado AK, Junqueira JC, Jorge AO (2011) Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study. Lasers Med Sci 26(3):341–348CrossRefPubMed
25.
Araujo NC, Fontana CR, Bagnato VS, Gerbi ME (2014) Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine. Lasers Med Sci 29(2):629–635CrossRefPubMed
26.
Melo MA, Rolim JP, Passos VF, Lima RA, Zanin IC, Codes BM et al (2015) Photodynamic antimicrobial chemotherapy and ultraconservative caries removal linked for management of deep caries lesions. Photodiagn Photodyn Ther 12(4):581–586CrossRef
27.
Coimbra FC, Salles MM, De Oliveira VC, Macedo AP, Da Silva CH, Pagnano VO et al (2016) Antimicrobial efficacy of complete denture cleansers. Am J Dent 29(3):149–153PubMed
28.
Marsh PD (2010) Microbiology of dental plaque biofilms and their role in oral health and caries. Dent Clin N Am 54(3):441–454CrossRefPubMed
29.
Sousa AS, Prates RA, de Santi ME, Lopes RG, Bussadori SK, Ferreira LR et al (2016) Photodynamic inactivation of Candida albicans biofilm: influence of the radiant energy and photosensitizer charge. Photodiagn Photodyn Ther 14:111–114CrossRef
30.
Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a common cause of persistent infections. Science 284(5418):1318–1322CrossRefPubMed
31.
Williams JA, Pearson GJ, Colles MJ, Wilson M (2003) The effect of variable energy input from a novel light source on the photoactivated bactericidal action of toluidine blue O on Streptococcus mutans. Caries Res 37(3):190–193CrossRefPubMed
32.
Costa AC, Chibebe Junior J, Pereira CA, Machado AK, Beltrame Junior M, Junqueira JC et al (2010) Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode. Braz Oral Res 24(4):413–418CrossRefPubMed
33.
Dovigo LN, Pavarina AC, Carmello JC, Machado AL, Brunetti IL, Bagnato VS (2011) Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin. Lasers Surg Med 43(9):927–934CrossRefPubMed
34.
Dovigo LN, Pavarina AC, Mima EG, Giampaolo ET, Vergani CE, Bagnato VS (2011) Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata. Mycoses 54(2):123–130CrossRefPubMed
35.
Quishida CC, Carmello JC, Mima EG, Bagnato VS, Machado AL, Pavarina AC (2015) Susceptibility of multispecies biofilm to photodynamic therapy using photodithazine(R). Lasers Med Sci 30(2):685–694CrossRefPubMed
36.
Ramage G, Vandewalle K, Wickes BL, Lopez-Ribot JL (2001) Characteristics of biofilm formation by Candida albicans. Rev Iberoam Micol 18(4):163–170PubMed
37.
Varoni E, Tarce M, Lodi G, Carrassi A (2012) Chlorhexidine (CHX) in dentistry: state of the art. Minerva Stomatol 61(9):399–419PubMed
38.
de Sousa Farias SS, Nemezio MA, Corona SA, Aires CP, Borsatto MC (2016) Effects of low-level laser therapy combined with toluidine blue on polysaccharides and biofilm of Streptococcus mutans. Lasers Med Sci 31(5):1011–1016CrossRefPubMed
39.
Ccahuana-Vasquez RA, Cury JA (2010) S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization. Braz Oral Res 24(2):135–141CrossRefPubMed
40.
Wilson M, Burns T, Pratten J (1996) Killing of Streptococcus sanguis in biofilms using a light-activated antimicrobial agent. J Antimicrob Chemother 37(2):377–381CrossRefPubMed
41.
Schneider M, Kirfel G, Berthold M, Frentzen M, Krause F, Braun A (2012) The impact of antimicrobial photodynamic therapy in an artificial biofilm model. Lasers Med Sci 27(3):615–620CrossRefPubMed
42.
Ricatto LG, Conrado LA, Turssi CP, Franca FM, Basting RT, Amaral FL (2014) Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: an in vitro study. Eur J Dent 8(4):509–514CrossRefPubMedPubMedCentral
43.
Wainwright M (2005) The development of phenothiazinium photosensitisers. Photodiagn Photodyn Ther 2(4):263–272CrossRef
44.
Usacheva MN, Teichert MC, Biel MA (2003) The interaction of lipopolysaccharides with phenothiazine dyes. Lasers Surg Med 33(5):311–319CrossRefPubMed
Metadaten
Titel
Influence of pre-irradiation time employed in antimicrobial photodynamic therapy with diode laser
verfasst von
Ana Caroline Fumes
Priscilla Coutinho Romualdo
Rachel Maciel Monteiro
Evandro Watanabe
Silmara Aparecida Milori Corona
Maria Cristina Borsatto
Publikationsdatum
30.09.2017
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 1/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-017-2336-1

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