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01.09.2006 | Original Paper

The Effect of Melatonin on TNBS-Induced Colitis

verfasst von: Ahmet Necefli, Burcu Tulumoğlu, Murat Giriş, Umut Barbaros, Mücteba Gündüz, Vakur Olgaç, Recep Güloğlu, Gülçin Toker

Erschienen in: Digestive Diseases and Sciences | Ausgabe 9/2006

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Abstract

Ulcerative colitis is a multifactorial inflammatory disease of the colon and rectum with an unknown etiology. The present study was undertaken to investigate the effect of melatonin administration on oxidative damage and apoptosis in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Rats were divided into four groups as follows: Group 1 (n=8)—TNBS colitis; Group 2 (n=8)—melatonin, 10 mg/kg/day ip, for 15 days in addition to TNBS; Group 3 (n=8)—melatonin alone, 10 mg/kg/day ip, for 15 days; and Group 4 (n=8)—isotonic saline solution, 1ml/rat ip, for 15 days (sham control group). Colonic myeloperoxidase (MPO) activities, malondialdehyde (MDA) levels, and glutathione (GSH) levels are indicators of oxidative damage, while caspase-3 activities reveal the degree of apoptosis of the colonic tissue. In all TNBS-treated rats, colonic MPO activity and MDA levels were found to be increased significantly compared to those in the sham group. Colonic MPO activity and MDA levels were significantly lower in the melatonin treatment group compared to TNBS-treated rats. GSH levels of colonic tissues were found to be significantly lower in TNBS-treated rats compared to the sham group. Treatment with melatonin significantly increased GSH levels compared to those in TNBS-treated rats. Caspas-3 activity of colonic tissues was found to be significantly higher in TNBS-treated rats compared to the sham group. Treatment with melatonin significantly decreased caspase-3 activity compared to that in TNBS-treated rats. These results imply a reduction in mucosal damage due to anti-inflammatory and anti-apoptotic effects of melatonin.

Guarner F, Malagelada JR (2003) Role of bacteria in experimental colitis. Best Pract Res Clin Gastroenterol 17(5):793–804PubMedCrossRef

Kankuri E, Hamalainen M, Hukkanen M, Salmenpera P, Kivilaakso E, Vapaatalo H, Moilanen E (2003) Suppresion of pro-inflamaory cytokine release by selective inhibition of inducible nitric oxide synthase in mucosal explants from patients with ulcerative colitis. Scand J Gastroenterol 38(2):186–192PubMedCrossRef

Stokkers P, Hommes D (2004) New cytokine therapeutics for inflammatory bowel disease. Cytokine 28:167–173PubMedCrossRef

Cho C (2001) Current roles of nitric oxide in gastrointestinal disorders. J Physiol 95:253–256

Danese S, Sans M, Fiocchi C (2004) Inflammatory bowel disease: the role of environmental factors. Aotuimmun Rev 3:394–400CrossRef

Shusterman T, Sela S, Cohen H, Kristal B, Sbeit W, Reshef R (2003) Effect of the antioxidant Mesna (2–mercaptoethane sulfonate) on experimental colitis. Dig Dis Sci 48(6):1177–1185PubMedCrossRef

Sugimoto K, Hanai H, Tozawa K, Aoshi T, Uchijima M, Nagata T, Koide Y (2002) Curcumin prevents and amoliorates trinitrobenzene sulfonic acid–induced colitis in mice. Gastroenterology 123(6):1912–1922PubMedCrossRef

Akin ML, Gulluoglu BM, Uluutku H, Erenoglu C, Elbuken E, Yildirim S, Celenk T (2002) Hyperbaric oxygen improves healing in experimental rat colitis. Undersea Hyperb Med 29(4):279–285PubMed

Andreadou I, Papalois A, Triantafillidis JK, Demonakou M, Govosdis V, Vidali M, Anagnostakis E, Kourounakis PN (2002) Benefical effects of a novel non-steroidal anti-inflammatory agent with basic character and antioxidant properties on experimental colitis in rats. Eur J Pharmacol 441(3):209–214PubMedCrossRef

10.

Woodruff TM, Arumugam TV, Shiels IA, Newman ML, Ross PA, Reid RC, Fairlie DP, Taylor SM (2005) A potent and selective inhibitor of group II a secretory phospholipase A2 protects rats from TNBS-induced colitis. Int Immunopharmacol 5:883–892PubMedCrossRef

11.

Pfeiffer CJ, Qiu B, Lam SK (2001) Reduction of colonic mucus by repeated short term stress enhances experimental colitis in rats. J Physiol 95:81–87

12.

Ademoğlu E, Erbil Y, Tam B, Barbaros U, İlhan E, Olgaç V, Türkoğlu UM (2004) Do vitamin E and selenium have beneficial effects on Trinitrobenzenesulfonic acid-induced experimental colitis. Dig Dis Sci 49:102–108PubMedCrossRef

13.

Jian YT, Mai GF, Wang JD, Zhang YL, Luo RC, Fang YX (2005) Preventive and therapeutic effects of NF-kappaB inhibitor curcumin in rats colitis induced by trinitrobenzene sulfonic acid. World J Gastroenterol 11:1747–1752PubMed

14.

Otegbayo JA, Otegbeye FM, Rotimi O (2005) Microscopic colitis syndrome—-a review article. J Natl Med Assoc 97(5):678–682PubMed

15.

Kazez A, Demirbağ M, Üstündağ B, Özercan İH, Sağlam M (2000) The role of melatonin in prevention of intestinal ischemia—Reperfusion injury in rats. J Pediatr Surg 35:1444–1448PubMedCrossRef

16.

Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, WallaceI JL (1989) Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology 96:795–803PubMed

17.

Millar AD, Rampton DS, Chander CL, Claxson AW, Blades S, Coumbe A, Panetta J, Morris CJ, Blake DR (1996) Evaluating the antioxidant potential of new treatments for inflammatory bowel disease using a rat model of colitis. Gut 39:407–415PubMedCrossRef

18.

Bradley PP, Priebat DA Christensen RD, Rothstein G (1982) Measurement of cutaneous inflammation estimation of neutrophil content with an enzyme marker. J Invest Dermatol 78:206–209PubMedCrossRef

19.

Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310PubMedCrossRef

20.

Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissues with Ellmann's reagent. Anal Biochem 25:192–205PubMedCrossRef

21.

Chang WK, Yang KD, Chuang H, Jan JT, Shaio MF (2002) Glutamine protects activated human T cells from apoptosis by up-regulating glutathione and Bcl-2 levels. Clin Immunol 104(2):151–160PubMedCrossRef

22.

Chang CK, Llanes S, Schumer W (1999) Inhibitory effect of dimethyl sulfoxide on nuclear factor-kappa B activation and intercellular adhesion molecule 1 gene expression in septic rats. J Surg Res 82:294–299PubMedCrossRef

23.

Jobin C, Sartor RB (2000) The I kappa B/NF-kappa B system: a key determinant of mucosal inflammation and protection. Am J Physiol Cell Physiol 278(3):451–462

24.

Schmid RM, Adler G (2000) NF-B/Rel/IkB: Implications in gastrointestinal diseases. Gastroenterology 118:1208–1226PubMedCrossRef

25.

Akdis CA, Blaser K, Akdis M (2004) Apoptosis in tissue inflammation and allergic disease. Curr Opin Immunol 16(6):717–723PubMedCrossRef

26.

Jones BA, Gores GJ (1997) Physiology and pathophysiology of apoptosis in epithelial cells of the liver, pancreas, and intestine. Am J Physiol 273:1174–1188

27.

Potten CS, Booth C (1997) The role of radiation-induced and spontaneous apoptosis in the homeostasis of the gastrointestinal epithelium: a brief review. Biochem Physiol Biochem Mol Biol 118(3):473–478CrossRef

28.

Potten CS, Wilson JW, Booth C (1997) Regulation and significance of apoptosis in the stem cells of the gastrointestinal epithelium. Stem Cells 15(2):82–93PubMedCrossRef

29.

Sun F, Hamagawa E, Tsutsui C, Sakaguchi N, Kakuta Y, Tokumaru S, Kojo S (2003) Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 65(1):101–107PubMedCrossRef

30.

Lee PP, Pang SF (1993) Melatonin and its receptors in the gastrointestinal tract. Biol Signals 2(4):181–193PubMedCrossRef

31.

Clausrat B, Brun J, Chazot G (2005) The basic physiology and pathophysiology of melatonin. Sleep Med Rev 9(1):11–24CrossRef

32.

Sileri P, Sica GS, Gentileschi P, Venza M, Benavoli D, Jarzembowski T, Manzelli A, Gaspari AL (2004) Melatonin reduces bacterial translocation after intestinal ischemia-reperfusion injury. Transplant Proc 36(10):2944–2946PubMedCrossRef

33.

Ozacmak VH, Sayan H, Arslan SO, Altaner S, Aktas RG (2005) Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum. Life Sci 18; 76(14):1575–1588CrossRef

34.

Bulbuller N, Dogru O, Yekeler H, Cetinkaya Z, Ilhan N, Kirkil C (2005) Effect of melatonin on wound healing in normal and pinealectomized rats. J Surg Res 123(1):3–7PubMedCrossRef

35.

Ates B, Yilmaz I, Geckil H, Iraz M, Birincioglu M, Fiskin K (2004) Protective role of melatonin given either before ischemia or prior to reperfusion on intestinal ischemia-reperfusion damage. J Pineal Res 37(3):149–152PubMedCrossRef

36.

Jahovic N, Sener G, Cevik H, Ersoy Y, Arbak S, Yegen BC (2004) Amelioration of methotrexate-induced enteritis by melatonin in rats. Cell Biochem Funct 22(3):169–178CrossRef

37.

Sener G, Jahovic N, Tosun O, Atasoy BM, Yegen BC (2003) Melatonin ameliorates ionizing radiation-induced oxidative organ damage in rats. Life Sci 74(5):563–572PubMedCrossRef

Titel: The Effect of Melatonin on TNBS-Induced Colitis
verfasst von: Ahmet Necefli
Burcu Tulumoğlu
Murat Giriş
Umut Barbaros
Mücteba Gündüz
Vakur Olgaç
Recep Güloğlu
Gülçin Toker
Publikationsdatum: 01.09.2006
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 9/2006
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-005-9047-3

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