Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 5/2011

01.05.2011 | Goanadal Physiology and Disease

The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population

verfasst von: Sonia Brahem, Meriem Mehdi, Hatem Elghezal, Ali Saad

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2011

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To investigate the effects of male aging on semen quality, DNA fragmentation and chromosomal abnormalities in the spermatozoa of infertile patients and fertile men.

Methods

Semen samples of 140 infertile patients (24–76 years) and 50 men with proven fertility (25–65 years) were analyzed according to WHO guidelines. DNA fragmentation was detected by TUNEL assay, while aneuploidy was assessed by FISH.

Results

In the patient group, semen volume and vitality of spermatozoa decreased significantly with age, while sperm concentration showed a statistically significant increase with age. DNA fragmentation as well as disomy of sex chromosomes and disomy 8 did not show a statistically significant change with age. However, the diploidy rate was significantly increased with patient’s age. In the control group, conventional semen parameters as well as DNA fragmentation and chromosomal abnormalities did not show a statistically significant with age.

Conclusion

Increased age in infertile men is associated with an increase in sperm concentration and diploidy, as well as a decline in semen volume and sperm vitality. However motility, morphology and DNA fragmentation are not affected by male age.
Literatur
1.
Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on semen quality and fertility: a review of the literature. Fertil Steril. 2001;75:237–48.PubMedCrossRef
2.
Eskenazi B, Wyrobek AJ, Sloter E, Kidd SA, Moore L, Young S, et al. The association of age and semen quality in healthy men. Hum Reprod. 2003;18:447–54.PubMedCrossRef
3.
Lewis SE, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 2005;322:33–41.PubMedCrossRef
4.
Benchaib M, Lornage J, Mazoyer C, Lejeune H, Salle B, François Guerin J. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril. 2007;87:93–100.PubMedCrossRef
5.
Bungum M, Humaidan P, Axmon A, Spano M, Bungum L, Erenpreiss J, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod. 2007;22:174–9.PubMedCrossRef
6.
Vagnini L, Baruffi RL, Mauri AL, Petersen CG, Massaro FC, Pontes A, et al. The effects of male age on sperm DNA damage in an infertile population. Reprod Biomed Online. 2007;15:514–9.PubMedCrossRef
7.
Spano M, Kolstad AH, Larsen SB, Cordelli E, Leter G, Giwercman A, et al. The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies. Hum Reprod. 1998;13:2495–505.PubMedCrossRef
8.
Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs EW, et al. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci USA. 2006;103:9601–6.PubMedCrossRef
9.
Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) its relationship fertilization embryo development. Hum Reprod. 2002;17:990–8.PubMedCrossRef
10.
Colin A, Barroso G, Gomez-Lopez N, Duran EH, Oehninger S. The effect of age on the expression of apoptosis biomarkers in human spermatozoa. Fertil Steril 2010 (in press).
11.
Schmid TE, Eskenazi B, Baumgartner A, Marchetti F, Young S, Weldon R, et al. The effects of male age on sperm DNA damage in healthy non-smokers. Hum Reprod. 2007;22:180–7.PubMedCrossRef
12.
Winkle T, Rosenbusch B, Gagsteiger F, Paiss T, Zoller N. The correlation between male age, sperm quality and sperm DNA fragmentation in 320 men attending a fertility center. J Assist Reprod Genet. 2009;26:41–6.PubMedCrossRef
13.
Hook EB, Schreinemachers DM, Willey AM, Cross PK. Rates of mutant structural chromosome rearrangements in human foetuses: data from prenatal cytogenetic studies and associations with maternal age and parental mutagen exposure. Am J Hum Genet. 1983;35:96–109.PubMed
14.
Nicolaidis P, Petersen MB. Origin and mechanisms of nondisjunction in human autosomal trisomies. Hum Reprod. 1998;13:313–9.PubMedCrossRef
15.
Sloter ED, Marchetti F, Eskenazi B, Weldon RH, Nath J, Cabreros D, et al. Frequency of human sperm carrying structural aberrations of chromosome 1 increases with advancing age. Fertil Steril. 2007;87:1077–86.PubMedCrossRef
16.
Luetjens CM, Rolf C, Gassner P, Werny JE, Nieschlag E. Sperm aneuploidy rates in younger and older men. Hum Reprod. 2002;17:1826–32.PubMedCrossRef
17.
World Health O. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge, UK, Published on behalf of the World Health Organization by Cambridge University Press; 1999.
18.
David G. Editorial: sperm banks in France. Arch Fr Pediatr. 1975;5:401–4.
19.
Schwartz D, Mayaux MJ, Spria A, Moscato ML, Jouannet P, Czyglik F, et al. Semen characteristics as a function of age in 833 fertile men. Fertil Steril. 1983;39:530–5.PubMed
20.
Levitas E, Lunenfeld E, Weisz N, Friger M, Potashnik G. Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6, 022 semen samples. Andrologia. 2007;39:45–50.PubMedCrossRef
21.
Rolf C, Behre HM, Nieschlag E. Reproductive parameters of older couples compared to younger men of infertile couples. Int J Androl. 1996;19:135–42.PubMedCrossRef
22.
Rolf C, Kenkel S, Nieschlag E. Age-related disease pattern in infertile men: increasing incidence of infections in older patients. Andrologia. 2002;34:209–17.PubMedCrossRef
23.
Andolz P, Bielsa MA, Vila J. Evolution of semen quality in northeastern Spain: a study in 22 759 infertile men over a 36 year period. Hum Reprod. 1999;14:731–5.PubMedCrossRef
24.
Mladenovic I, Micic S, Papic N, Genbacev O, Marinkovic B. Sperm morphology and motility in different age populations. Arch Androl. 1994;32:197–205.PubMedCrossRef
25.
Auger J, Kunstmann JM, Czyglik F, Jouannet P. Decline in semen quality among fertile men in Paris during the past 20 years. N Engl J Med. 1995;332:281–5.PubMedCrossRef
26.
Fisch H, Goluboff ET, Olson JH, Feldshuh J, Broder SJ, Barad DH. Semen analysis in 1, 283 men from the United States over a 25-year period: no decline in quality. Fertil Steril. 1996;65:1009–14.PubMed
27.
Bujan L, Mansat A, Pontonnier F, Mieusset R. Time series analysis of sperm concentration in fertile men in Toulouse, France between 1977 and 1992. BMJ. 1996;312:471–2.PubMed
28.
Irvine S, Cawood E, Richardson D, MacDonald E, Aitken J. Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in Scotland over 11 years. BMJ. 1996;312:467–71.PubMed
29.
Plastira K, Msaouel P, Angelopoulou R, Zanioti K, Plastiras A, Pothos A, et al. The effects of age on DNA fragmentation, chromatin packaging and conventional semen parameters in spermatozoa of oligoasthenoteratozoospermic patients. J Assist Reprod Genet. 2007;24:437–43.PubMedCrossRef
30.
Sailer BL, Jost LK, Evenson DP. Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal desoxynucleotidyl transferase assay. J Androl. 1995;16:80–7.PubMed
31.
Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. CMAJ. 2006;175:495–500.PubMedCrossRef
32.
Agarwal A, Saleh RA. Utility of oxidative stress test in the male infertility clinic. Zhonghua Nan Ke Xue. 2002;8:1–9.PubMed
33.
34.
Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod. 1999;4:31–7.PubMedCrossRef
35.
Singh NP, Muller CH, Berger RE. Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil Steril. 2003;80:1420–30.PubMedCrossRef
36.
Sati L, Ovari L, Bennett D, Simon SD, Demir R, Huszar G. Double probing of human spermatozoa for persistent histones, surplus cytoplasm, apoptosis and DNA fragmentation. Reprod Biomed Online. 2008;16:570–9.PubMedCrossRef
37.
Moskovtsev SI, Willis J, Mullen JB. Age-related decline in sperm deoxyribonucleic acid integrity in patients evaluated for male infertility. Fertil Steril. 2006;85:496–9.PubMedCrossRef
38.
Moskovtsev SI, Willis J, White J, Mullen JB. Sperm DNA damage: correlation to severity of semen abnormalities. Urology. 2009;74:789–93.PubMedCrossRef
39.
Bosch M, Rajmil O, Martinez-Pasarell O, Egozcue J, Templado C. Linear increase of diploidy in human sperm with age: a four-colour FISH study. Eur J Hum Genet. 2001;9:533–8.PubMedCrossRef
40.
Bosch M, Rajmil O, Egozcue J, Templado C. Linear increase of structural and numerical chromosome 9 abnormalities in human sperm regarding age. Eur J Hum Genet. 2003;11:754–9.PubMedCrossRef
41.
Griffin DK, Abruzzo MA, Millie EA, Sheean LA, Feingold E, Sherman SL, et al. Non-disjunction in human sperm: evidence for an effect of increasing paternal age. Hum Mol Gen. 1995;4:2227–32.PubMedCrossRef
42.
Robbins WA, Baulch JE, Moore DI, Weire HU, Blakey D, Wyrobek AJ. Three-probe fluorescence in situ hybridization to assess chromosome X, Y, and 8 aneuploidy in sperm of 14 men from two healthy groups: evidence for a paternal age effect on sperm aneuploidy. Reprod Fertil Dev. 1995;7:799–809.PubMedCrossRef
43.
Martin RH, Spriggs E, Ko E, Rademaker AW. The relationship between paternal age, sex ratios, and aneuploidy frequencies in human sperm, as assessed by multicolor FISH. Am J Hum Gen. 1995;57:1395–9.
44.
Kinakin B, Rademaker A, Martin R. Paternal age effect of YY aneuploidy in human sperm, as assessed by fluorescence in situ hybridization. Cytogenet Cell Genet. 1997;78:116–9.PubMedCrossRef
45.
Asada H, Sueoka K, Hashiba T, Kuroshima M, Kobayashi N, Yoshimura Y. The effects of age and abnormal sperm count on the nondisjunction of spermatozoa. J Assist Reprod Genet. 2000;17:51–9.PubMedCrossRef
46.
Guttenbach M, Kohn FM, Engel W, Schmid M. Meiotic nondisjunction of chromosomes 1, 17, 18, X, and Y in men more than 80 years of age. Biol Reprod. 2000;63:1727–9.PubMedCrossRef
47.
Lowe X, Eskenazi B, Nelson DO, Kidd S, Alme A, Wyrobek AJ. Frequency of XY sperm increases with age in fathers of boys with Klinefelter syndrome. Am J Hum Genet. 2001;69:1046–54.PubMedCrossRef
Metadaten
Titel
The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population
verfasst von
Sonia Brahem
Meriem Mehdi
Hatem Elghezal
Ali Saad
Publikationsdatum
01.05.2011
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 5/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-011-9537-5

Weitere Artikel der Ausgabe 5/2011

Journal of Assisted Reproduction and Genetics 5/2011 Zur Ausgabe

Assisted Reproduction Technologies

Are poor responders patients at higher risk for producing aneuploid embryos in vitro?

EDITOR'S COMMENTARY

Nanosurgical interventions in the practice of human ARTs

Fertility Preservation

The human postmenopausal ovary as a tool for evaluation of cryopreservation protocols towards whole ovary cryopreservation

Assisted Reproduction Technologies

The impact of environmental exposure to perfluorinated compounds on oocyte fertilization capacity

Gamete Biology

Is sperm dna damage associated with IVF embryo quality? A systematic review

Technical Innovations

Use of parthenogenetic activation of human oocytes as an experimental model for evaluation of polar body based PGD assay performance

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

26.04.2024 Hirsutismus Nachrichten

Laser- und Lichtbehandlungen können bei Frauen mit polyzystischem Ovarialsyndrom (PCOS) den übermäßigen Haarwuchs verringern und das Wohlbefinden verbessern – bei alleiniger Anwendung oder in Kombination mit Medikamenten.

ICI-Therapie in der Schwangerschaft wird gut toleriert

23.04.2024 Medikamente in Schwangerschaft und Stillzeit Nachrichten

Müssen sich Schwangere einer Krebstherapie unterziehen, rufen Immuncheckpointinhibitoren offenbar nicht mehr unerwünschte Wirkungen hervor als andere Mittel gegen Krebs.

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

23.04.2024 Depression antepartal oder postpartal Nachrichten

Bislang gibt es kein Medikament zur Prävention von Wochenbettdepressionen. Das Injektionsanästhetikum Esketamin könnte womöglich diese Lücke füllen.

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

22.04.2024 DGIM 2024 Kongressbericht

Durch die Häufung nach der COVID-19-Pandemie sind Infektionen mit dem Respiratorischen Synzytial-Virus (RSV) in den Fokus gerückt. Fachgesellschaften empfehlen eine Impfung inzwischen nicht nur für Säuglinge und Kleinkinder.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen
Bildnachweise