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01.03.2007

Effects of Tumour Necrosis Factor-α on Human Sperm Motility and Apoptosis

verfasst von: Anna Perdichizzi, Ferdinando Nicoletti, Sandro La Vignera, Nunziata Barone, Rosario D’Agata, Enzo Vicari, Aldo E. E. Calogero

Erschienen in: Journal of Clinical Immunology | Ausgabe 2/2007

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Abstract

The aim of this study was to evaluate the effects of tumour necrosis factor-α (TNF-α) on sperm motility, mitochondrial membrane potential (ΔΨ), phosphatidylserine (PS) externalization, sperm chromatin packaging quality, and DNA fragmentation. Motile spermatozoa, obtained from 10 normozoospermic men, were incubated with increasing concentrations of TNF-α and analyzed 1, 3, 6, and 24 h after incubation by flow cytometry. TNF-α decreased total motility 24 h after incubation at 10 ng/mL and progressive motility 3 h after incubation. Accordingly, TNF-α reduced sperm ΔΨ in a concentration- and time-dependent manner. TNF-α increased the percentage of spermatozoa with PS externalization from the concentration of 1 ng/mL 1 h after incubation. TNF-α produced sperm chromatin and DNA damage in a concentration- and time-dependent manner. In conclusion, these findings may explain the reduction of fertility, secondary to upregulated production of TNF-α, in men with urogenital infections.

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Titel: Effects of Tumour Necrosis Factor-α on Human Sperm Motility and Apoptosis
verfasst von: Anna Perdichizzi
Ferdinando Nicoletti
Sandro La Vignera
Nunziata Barone
Rosario D’Agata
Enzo Vicari
Aldo E. E. Calogero
Publikationsdatum: 01.03.2007
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 2/2007
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-007-9071-5

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