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03.02.2021 | Research Article

Multimodality In Vivo Imaging of Perfusion and Glycolysis in a Rat Model of C6 Glioma

verfasst von: Qi Qi, Matthew S. Fox, Heeseung Lim, Robert Bartha, Timothy J. Scholl, Lisa Hoffman, Ting-Yim Lee, Jonathan D. Thiessen

Erschienen in: Molecular Imaging and Biology | Ausgabe 4/2021

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Abstract

Purpose

Chemical exchange saturation transfer MRI using an infusion of glucose (glucoCEST) is sensitive to the distribution of glucose in vivo; however, whether glucoCEST is more related to perfusion or glycolysis is still debatable. We compared glucoCEST to computed tomography perfusion (CTP), [¹⁸F] fluorodeoxyglucose positron emission tomography (FDG-PET), and hyperpolarized [1-¹³C] pyruvate magnetic resonance spectroscopy imaging (MRSI) in a C6 rat model of glioma to determine if glucoCEST is more strongly correlated with measurements of perfusion or glycolysis.

Methods

10⁶ C6 glioma cells were implanted in Wistar rat brains (n = 11). CTP (including blood volume, BV; blood flow, BF; and permeability surface area product, PS) and FDG-PET standardized uptake value (SUV) were acquired at 11 to 13 days post-surgery. GlucoCEST measurements (∆CEST) were acquired the following day on a 9.4 T MRI before and after an infusion of glucose solution. This was followed by MRSI on a 3.0 T MRI after the injection of hyperpolarized [1-¹³C] pyruvate to generate regional maps of the lactate:pyruvate ratio (Lac:Pyr). Pearson’s correlations between glucoCEST, CTP, FDG-PET, and Lac:Pyr ratio were evaluated.

Results

Tumors had significantly higher SUV, BV, and PS than the contralateral brain. Tumor ∆CEST was most strongly correlated with CTP measurements of BV (ρ = 0.74, P = 0.01) and PS (ρ = 0.55, P = 0.04). No significant correlation was found between glycolysis measurements of SUV or Lac:Pyr with tumor ∆CEST. PS significantly correlated with SUV (ρ = 0.58, P = 0.005) and Lac:Pyr (ρ = 0.75, P = 0.005). BV significantly correlated with Lac:Pyr (ρ = 0.57, P = 0.02), and BF significantly correlated with SUV (ρ = 0.49, P = 0.02).

Conclusion

This study determined that glucoCEST is more strongly correlated to measurements of perfusion than glycolysis. GlucoCEST measurements have additional confounds, such as sensitivity to changing pH, that merit additional investigation.

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Titel: Multimodality In Vivo Imaging of Perfusion and Glycolysis in a Rat Model of C6 Glioma
verfasst von: Qi Qi
Matthew S. Fox
Heeseung Lim
Robert Bartha
Timothy J. Scholl
Lisa Hoffman
Ting-Yim Lee
Jonathan D. Thiessen
Publikationsdatum: 03.02.2021
Verlag: Springer International Publishing
Erschienen in: Molecular Imaging and Biology / Ausgabe 4/2021
Print ISSN: 1536-1632
Elektronische ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-021-01585-1

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Multimodality In Vivo Imaging of Perfusion and Glycolysis in a Rat Model of C6 Glioma