nach oben

Journal of Natural Medicines

Erschienen in:

01.07.2013 | Note

Application of quantitative ¹H-NMR method to determination of paeoniflorin in Paeoniae radix

verfasst von: Rie Tanaka, Marina Yamazaki, Keiko Hasada, Akito Nagatsu

Erschienen in: Journal of Natural Medicines | Ausgabe 3/2013

Einloggen, um Zugang zu erhalten

Abstract

A quantitative ¹H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45 %. We demonstrated that this method is useful for quantitative analysis of crude drugs.

Shibata S, Nakahara M (1963) Studies on the constituents of Japanese and Chinese crude drugs. VIII. Paeoniflorin, a glucoside of Chinese paeony root. Chem Pharm Bull 11(3):372–378CrossRef

Harada M (1989) Experimental methodology on pharmacological studies on crude drugs. J Tradit Sino Jpn Med 107:1–16

Hikino H (1985) Oriental medical plants. In: Wagner H, Hikino H, Farnsworth NR (eds) Economic and medicinal plant research, vol. 1. London Academic Press, London

Yamahara J, Yamada T, Kimura H, Sawada T, Fujimura H (1982) Biologically active principles of crude drugs. II. Anti-allergic principles in “Shoseiryu-To” anti-inflammatory properties of paeoniflorin and its derivatives. J Pharmacobiodyn 5(11):921–929PubMedCrossRef

Takagi K, Harada M (1969) Pharmacological studies on herb paeony root. II. Anti-inflammatory effect, inhibitory effect on gastric juice secretion, preventive effect on stress ulcer, antidiuretic effect of paeoniflorin and combined effects with licorice component Fm 100. Yakugaku Zasshi 89(7):887–892PubMed

Sugishita E, Amagaya S, Ogihara Y (1984) Studies on the combination of Glycyrrhizae Radix in Shakuyakukanzo-To. J Pharmacobiodyn 7(7):427–435PubMedCrossRef

Ministry of Health and Welfare (2000) The Japanese Pharmacopeia, 15th edn. Japan, pp 1223–1224

Pauli GF, Jaki BU, Lankin DC (2005) Quantitative ¹H NMR: development and potential of a method for natural products analysis. J Nat Prod 68:133–149PubMedCrossRef

Tahara M, Sugimoto N, Suematsu T, Arifuku K, Saito T, Ihara T, Yoshida Y, Tada A, Kubota R, Shimizu K, Yamazaki T, Tanamoto K, Nakazawa H, Nishimura T (2009) Quality control of organophosphorous isoxathion oxon based on qNMR. Jpn J Food Chem Safety 16:28–33

10.

Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2010) Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by ¹H-NMR spectrometry. J Nat Med 64:161–164PubMedCrossRef

11.

Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2011) Application of ¹H-NMR spectroscopy to validation of berberine alkaloid reagents and to chemical evaluation of Coptidis Rhizoma. J Nat Med 65:262–267PubMedCrossRef

12.

Saito T, Ihara T, Koike M, Kinugasa S, Fujimine Y, Nose K, Hirai T (2009) A new traceability scheme for the development of international system-traceable persistent organic pollutant reference materials by quantitative nuclear magnetic resonance. Accredit Qual Assur 14:79–86CrossRef

13.

Yoo J-S, Song M-C, Ahn E-M, Lee Y-H, Rho Y-D, Baek N-I (2006) Quantitative analysis of paeoniflorin from Paeonia lactiflora using ¹H-NMR. Nat Prod Sci 12(4):237–240

Titel: Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix
verfasst von: Rie Tanaka
Marina Yamazaki
Keiko Hasada
Akito Nagatsu
Publikationsdatum: 01.07.2013
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 3/2013
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-012-0711-6

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2013

Application of NIR spectroscopy for the quality control of mangosteen pericarp powder: quantitative analysis of alpha-mangostin in mangosteen pericarp powder and capsule

Tricin 4′-O-(erythro-β-guaiacylglyceryl) ether and tricin 4′-O-(threo-β-guaiacylglyceryl) ether isolated from Njavara (Oryza sativa L. var. Njavara), induce apoptosis in multiple tumor cells by mitochondrial pathway

Medicinal parasitic plants on diverse hosts with their usages and barcodes

Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel

New cholestane glycosides and sterols from the underground parts of Chamaelirium luteum and their cytotoxic activity

Anti-degranulating activity in rat basophil leukemia RBL-2H3 cells of flavanone glycosides and their aglycones in citrus fruits