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Journal of Natural Medicines

Erschienen in:

01.01.2014 | Note

Application of a quantitative ¹H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus

verfasst von: Rie Tanaka, Akane Nitta, Akito Nagatsu

Erschienen in: Journal of Natural Medicines | Ausgabe 1/2014

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Abstract

A quantitative ¹H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d ₅ to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13 % in 2 lots of Persicae semen, 3.62 and 5.19 % in 2 lots of Armeniacae semen, and 0.23 % in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs.

Fukuda T, Ito H, Yoshida T (2003) Glycosides and their quantitative HPLC analysis of commercial Persicae semen. Nat Med 57(1):18–22

Miyagoshi M, Amagaya S, Ogihara Y (1986) Antitussive effects of L-ephedrine, amygdalin, and Makyokansekito (Chinese traditional medicine) using a cough model induced by sulfur dioxide gas in mice. Planta Med 52(4):275–278CrossRef

Hwang H-J, Kim P, Kim C-J, Lee HJ, Shim I, Yin CS, Yang Y, Hahm DH (2008) Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull 31:1559–1564PubMedCrossRef

Fukuda T, Ito H, Mukainaka T, Tokuda H, Nishino H, Yoshida T (2003) Anti-tumor promoting effect of glycosides from Prunus persica seeds. Biol Pharm Bull 26:271–273PubMedCrossRef

Mirmiranpour H, Khaghani S, Zandieh A, Khalilzadeh OO, Gerayesh-Nejad S, Morteza A, Esteghamati A (2012) Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats. Indian J Pathol Microbiol 55:211–214PubMedCrossRef

Chang HK, Shin MS, Yang HY, Lee JW, Kim YS, Lee MH, Kim J, Kim KH, Kim CJ (2006) Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCap prostate cancer cells. Biol Pharm Bull 29:1597–1602PubMedCrossRef

Ministry of Health and Welfare (2000) The Japanese Pharmacopoeia, 15th edn. Japan, pp 1223–1224

Pauli GF, Jaki BU, Lankin DC (2005) Quantitative ¹H NMR: development and potential of a method for natural products analysis. J Nat Prod 68:133–149PubMedCrossRef

Tahara M, Sugimoto N, Suematsu T, Arifuku K, Saito T, Ihara T, Yoshida Y, Tada A, Kubota R, Shimizu K, Yamazaki T, Tanamoto K, Nakazawa H, Nishimura T (2009) Quality control of organophosphorus pesticide isoxathion oxon based on qNMR. Jpn J Food Chem Safety 16:28–33

10.

Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2010) Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by ¹H-NMR spectroscopy. J Nat Med 64:161–166PubMedCrossRef

11.

Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2011) Application of ¹H-NMR spectroscopy to validation of berberine alkaloid reagents and to chemical evaluation of Coptidis Rhizoma. J Nat Med 65:262–267PubMedCrossRef

12.

Tanaka R, Yamazaki M, Hasada K, Nagatsu A (2012) Application of quantitative ¹H-NMR method to determination of paeoniflorin in Paeoniae radix. J Nat Med (in press)

13.

Saito T, Ihara T, Koike M, Kinugasa S, Fujimine Y, Nose K, Hirai T (2009) A new traceability scheme for the development of international system-traceable persistent organic pollutant reference materials by quantitative nuclear magnetic resonance. Accred Qual Assur 14:79–86CrossRef

14.

Ministry of Health and Welfare (2011) The Japanese Pharmacopeia, 16th edn. Japan, pp 1480–1481

15.

Seigler DS, Pauli GF, Nahrstedt A, Leen R (2002) Cyanogenic allosides and glucosides from Passiflora edulis and Carica papaya. Phytochemistry 60(8):873–882PubMedCrossRef

16.

Someya K, Mikoshiba S, Okumura T, Takenaka H, Ohdera M, Shirota O, Kuroyanagi M (2006) Suppressive effect of constituents isolated from kernel of Prunus armeniaca on 5 α-androst-16-en-3-one generated by microbial metabolism. J Oleo Sci 55(7):353–364CrossRef

17.

Cervellati C, Paetz C, Dondini L, Tartarini S, Bassi D, Schneider B, Masia A (2012) A qNMR approach for bitterness phenotyping and QTL identification in an F1 apricot progeny. J Biotechnol 159:312–319PubMedCrossRef

Titel: Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus
verfasst von: Rie Tanaka
Akane Nitta
Akito Nagatsu
Publikationsdatum: 01.01.2014
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 1/2014
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-013-0783-y

Springer Medizin

Abstract

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