Background
Methods
Strain collection
Origin | No. of isolates | No. of MLST types | AmpR | PCR IS16+ | PCR esp+ | PCR hyl
Efm
+
| PCR vanA+/B+
|
---|---|---|---|---|---|---|---|
Blood cultures | 160 | 32 | 98.1% (n = 157) | 96.9% (n = 155) | 63.8% (n = 102) | 45.6% (n = 73) | 58.1% (n = 93) |
Hosp.-associated E. faecium (CC17)
1
|
155
|
28
|
100% (n = 156)
|
100% (n = 156)
|
65.8% (n = 102)
|
46.5% (n = 72)
|
58.7% (n = 91)
|
Human commensal | 68 | 46 | 2.9% (n = 2) | 4.4% (n = 3)2
| 0% | 0% | 14.7% (n = 10) |
Animal commensal and meat sources | 32 | 22 | 6.2% (n = 2) | 0% | 0% | 0% | 40.6% (n = 13) |
DNA isolation
PCR
PFGE typing
Southern hybridizations
MLST and DNA sequencing
Statistics
Results
Identification of IS16primer sequences
Screening for markers in the two sets of strains
MLST typing
Southern hybridizations of IS16probes
Strain no. | Origin | MLST type |
vangenotype | I-CeuI PFGE | Plasmid |
---|---|---|---|---|---|
UW 6714 | BC |
202
| - | -* | + |
UW 1245 | BC |
202
|
vanA
| - | + |
UW 6293 | BC |
202
|
vanB
| -* | -#
|
UW 6295 | BC |
202
|
vanB
| -* | -#
|
UW 4962 | BC |
203
|
vanA
| + | -#
|
UW 6989 | BC |
203
| - | + | -#
|
UW 6502 | BC |
208
|
vanB
| + | + |
UW 5918 | BC |
252
|
vanA
| + | + |
UW 4675 | BC |
252
|
vanB
| + | - |
UW 6617 | BC |
275
|
vanA
| - | + |
UW 4960 | BC |
279
|
vanB
| + | - |
UW 6033 | BC |
282
|
vanA
| + | + |
UW 4512 | BC |
294
| - | + | + |
UW 6297 | BC |
306
| - | + | + |
UW 6935 | BC |
371
| - | + | + |
UW 3142 | BC |
375
|
vanA
| + | + |
UW 5275 | BC |
376
|
vanB
| + | + |
UW 6983 | BC |
377
| - | + | + |
UW 6112 | BC |
378
| - | + | + |
UW 4208 | BC |
New
| - | + | + |
UW 6642 | BC |
New
|
vanA
| + | + |
AK-EM 40 | HC |
18
| - | + | + |
AK-EM 53 | HC |
413
| - | + | + |
K226.7 | HC |
18
| - | + | - |
UW 2055 | BC |
16
|
vanA
| -* | + |
UW 3695 | BC |
17
| - | + | + |
UW 3544 | BC |
17
|
vanA
| + | -#
|
UW 5352 | BC |
17
|
vanB
| - | + |
UW 6990 | BC |
18
| - | + | + |
UW 1952 | BC |
18
|
vanA
| + | + |
UW 2900 | BC |
18
|
vanB
| + | -#
|
UW 1218 | BC |
19
|
vanA
| + | -#
|
UW 3056 | BC |
65
| - | + | + |
UW 6880 | BC |
78
| - | + | + |
UW 4671 | BC |
78
|
vanA
| + | - |
UW 2457 | BC |
80
|
vanA
| + | - |
UW 6985 | BC |
81
| - | - | + |
UW 2771 | BC |
81
|
vanA
| + | - |
UW 6882 | BC |
117
| - | + | - |
UW 1983 | BC |
117
|
vanA
| + | - |
UW 6805 | BC |
125
|
vanA
| + | + |
UW 2824 | BC |
132
|
vanA
| + | -#
|
UW 1716 | BC |
192
| - | + | -#
|
UW 5852 | BC |
192
|
vanA
| + | + |
UW 5267 | BC |
192
|
vanB
| + | -#
|