Background
Methods
Tissue samples
DNA extraction
Polymerase chain reaction amplification and product purification
Primer
Mutation analysis
Survival
Statistical analysis
Results
Patients' Characteristics
Number (%) | |
---|---|
Number of patients | 101 (100) |
Sex | |
Male | 59 (58.4) |
Female | 42 (41.6) |
Sub-localization | |
Colon | 54 (53.5) |
Rectum | 47 (46.5) |
Adenocarcinoma | |
Tubular/papillary | 32 (31.7) |
Mucinous | 12 (11.9) |
Others | 57 (56.4) |
Differentiation | |
Good | 2 (2.0) |
Moderate | 69 (68.3) |
Poor | 11 (10.9) |
Unknown | 19 (18.8) |
Stage (UICCa) | |
I | 7 (6.9) |
II | 20 (19.8) |
III | 49 (48.5) |
IV | 25 (24.8) |
Epidermal growth factor receptor and K-ras gene mutation types
Site | Wild type | Type of point mutation | Number of mutations (%) | Amino acid | Heterozygous/homozygous | |
---|---|---|---|---|---|---|
EGFRa [SME1] | Exon 18 | AAG | 2183A>G | 1 (33.3) | Gln849Leu | Heterozygous |
Exon 21 | CAG | 2546A>T | 1 (33.3) | Lys728Arg | Heterozygous | |
Exon 21 | GCA | 2611G>A | 1 (33.3) | Ala871Thr | Heterozygous | |
K-ras | Codon 12 | GGT | 35G>A | 16 (47.1) | Gly12Asp | Heterozygous |
Codon 12 | GGT | 34G>T | 6 (17.6) | Gly12Cys | Heterozygous | |
Codon 12 | GGT | 34G>A | 3 (8.8) | Gly12Ser | Heterozygous | |
Codon 12 | GGT | 34G>A | 1 (2.9) | Gly12Ser | Homozygous | |
Codon 12 | GGT | 34G>T | 1 (2.9) | Gly12Cys | Homozygous | |
Codon 12 | GGT | 35G>C | 1 (2.9) | Gly12Ala | Heterozygous | |
Codon 12 | GGT | 35G>A | 1 (2.9) | Gly12Asp | Homozygous | |
Codon 12 | GGT | 35G>T | 1 (2.9) | Gly12Val | Heterozygous | |
Codon 13 | GGC | 38G>A | 1 (2.9) | Gly13Asp | Heterozygous | |
Codon 45 | GTA | 133G>A | 1 (2.9) | Val45Ile | Homozygous | |
Codon 69 | GAC | 205G>A | 1 (2.9) | Asp69Asn | Homozygous | |
Codon 80 | TGT | 239G>A | 1 (2.9) | Cys80Tyr | Heterozygous |
Clinicopathological factors correlated with K-ras mutations
K-ras mutation status |
p Value | ||
---|---|---|---|
Mutation Number of patients | Wild type Number of patients | ||
Number of patients | 33 | 68 | |
Sex | 0.16 | ||
Male | 16 | 43 | |
Female | 17 | 25 | |
Age (years) | 0.89 | ||
≤56 | 17 | 34 | |
>56 | 16 | 34 | |
Sub-localization | 0.26 | ||
Colon | 15 | 39 | |
Rectum | 18 | 29 | |
Adenocarcinoma | 0.84 | ||
Tubular/papillary | 10 | 22 | |
Others | 23 | 46 | |
Differentiation | 0.05 | ||
Good | 2 | 0 | |
Moderate | 18 | 51 | |
Poor | 4 | 7 | |
Unknown | 9 | 10 | |
Stage (UICCa) | 0.18 | ||
I + II | 6 | 21 | |
III + IV | 27 | 47 |