Background
Results and discussion
No. | Sex | Age (years) | ALAT (IU/l) | GGTP (IU/l) | Hist-pat | Cryoglob. | Genotype | Negative strand HCV RNA | NS3 positive cells (%) | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CD3+ | CD14+ | CD19+ | CD3+ | CD14+ | CD19+ | ||||||||
1 | F | 61 | 31 | 53 | G9, S2 |
+
| 1b |
-
|
+
|
-
| 17 |
-
| - |
2 | M | 52 | 35 | nd | nd | - | 1b |
-
|
-
|
-
|
-
| - |
-
|
3 | M | 49 | 55 | 28 | Cirrhosis | - | 1b |
-
|
-
|
-
| 8 |
-
| - |
4 | M | 59 | 81 | 58 | G4, S3 | - | 1b |
-
|
-
|
-
|
-
| - |
-
|
5 | F | 66 | 36 | 54 | Cirrhosis |
+
| 1b |
-
|
-
|
-
| 7 | 1 | 1 |
6 | F | 63 | 55 | 29 | G4, S2 |
+
| 1b |
-
|
-
|
+
|
-
| - |
-
|
7 | F | 69 | 63 | 157 | nd |
+
| 1b |
-
|
-
|
-
| 4 | - | - |
8 | F | 59 | 77 | 121 | nd | - | 1b |
-
|
-
|
-
|
-
| - |
-
|
9 | F | 54 | 211 | 39 | nd | - | 1b |
+
|
-
|
-
| 1 | - | 2 |
10 | F | 51 | 64 | 81 | G9, S2 |
+
| 1b |
+
|
-
|
-
|
-
| - |
-
|
11 | M | 50 | 50 | 85 | Cirrhosis |
+
| 3a |
-
|
-
|
-
| 1 | - | - |
12 | F | 59 | 88 | 37 | G5, S2 |
+
| 1b |
-
|
-
|
-
|
-
| 2 | 4 |
13 | F | 54 | 71 | 91 | Cirrhosis | - | 1b |
-
|
-
|
-
|
-
| - |
-
|
14 | F | 62 | 116 | 64 | G5, S2 |
+
| 1b |
-
|
+
|
-
| 2 | 2 | 1 |
15 | F | 58 | 158 | 58 | G8, S2 | - | 1b |
-
|
-
|
-
|
-
| - |
-
|
16 | M | 58 | 35 | 133 | G7, S2 | - | 1b |
-
|
-
|
-
|
-
| 2 |
-
|
17 | F | 62 | 26 | 97 | Cirrhosis | - | 1b |
-
|
-
|
-
| 9 |
-
| 23 |
18 | M | 52 | 2165 | 401 | acute h. | - | 1b |
-
|
-
|
-
| 5 | 1 | - |
19 | M | 30 | 147 | 142 | G8, S2 | - | 1b |
-
|
-
|
+
|
-
| - |
-
|
20 | F | 64 | 307 | 140 | Cirrhosis |
+
| 1b |
-
|
-
|
-
|
-
| - |
-
|
21 | F | 58 | 63 | 109 | Cirrhosis | - | 1b |
-
|
-
|
-
| 8 | - | - |
22 | F | 65 | 79 | 24 | G4, S4 |
+
| 1b |
-
|
-
|
-
|
-
| - | 1 |
23 | M | 34 | 90 | 54 | G5, S1 | - | 1b |
-
|
-
|
-
| 1 | 1 | - |
24 | M | 28 | 105 | 28 | G5, S1 | - | 3a |
-
|
-
|
-
|
-
|
-
|
-
|
25 | F | 42 | 44 | 80 | Cirrhosis | - | 1b |
+
|
-
|
-
|
-
|
-
|
-
|
26 | F | 63 | 26 | nd | nd |
+
| 1b |
-
|
+
|
-
|
-
|
-
|
-
|
Conclusions
Methods
Magnetic cells sorting
Detection of negative strand HCV RNA
Sequences of primers | PCR conditions | |
---|---|---|
Reverse transcription
| 5′-TGR TGC ACG GTC TAC GAG ACC TC-3′ | Temp. 72°C/30 min |
I PCR
| 5′-RAYCACTCCCCTGTGAGGAAC-3′ | 1 min at 94°C; |
50 cycles of 94°C for 15 s, 58°C for 30 s, and 72°C for 30 s; 72°C for 7 min | ||
II PCR
| 5′-ACTGTCTTCACGCAGAAAGCGTC-3′ | 1 min at 94°C; |
5′-CAAGCACCCTATCAGGCAGTACC-3′ | 30 cycles of 94°C for 15 s, 58°C for 1 min, and 72°C for 1 min; at 72°C for 7 min |