Background
Results
CDV cytotoxicity, effective concentration for abolishing VV plaque formation
Virus | Parental virus strain | Original mutation in E9L | E9L sequence of reconstructed virus |
---|---|---|---|
CDVR 1 | VV WR | A314V | A314V |
CDVR 2 | VV TK::GFP | A314V | A314V |
CDVR 11 | VV TK::GFP, line 11 | A314V; P738S | ND |
CDVR 14 | VV WR, line 14 | A314V | ND |
CDVR 15 | VV WR, line 15 | M671I | M671I, ΔK174 |
CDVR 16 | VV WR, line 16 | ΔK174 | ΔK174 |
Marker rescue and mapping of mutations conferring resistance
Growth properties of CDVR viruses
Levels of resistance
Virus | HFF EC50 (μM)a | Vero EC50 (μM)a | Fold resistance over parental strain (HFF) | Fold resistance over parental strain (Vero) |
---|---|---|---|---|
VV-WR, UAB | 28 ± 4.4 | 62 ± 12 | - | - |
VV-WR, Moyer | 18 ± 9.2 | 54 ± 2.9 | - | - |
CDVR 1A | 122 ± 69 | >317 ± 0 | 7 | >6 |
CDVR 15A | 98 ± 55 | 214 ± 17 | 5 | 4 |
CDVR 16A | 49 ± 4.5 | 199 ± 2.8 | 3 | 4 |
Virulence of CDVR viruses in mice
Mortality | ||||
---|---|---|---|---|
Virusa | Number | Percent | MDDb | LD90 |
VV-WR, UAB
c
| ||||
2.8 × 104 | 10/10 | 100 | 7.1 | 2.9 × 103 |
2.8 × 103 | 7/10 | 70 | 8.1 | -- |
2.8 × 102 | 2/10 | 20 | 9.0 | -- |
28 | 0/10 | 0 | -- | -- |
2.8 | 0/10 | 0 | -- | -- |
VV-WR, Moyer
c
| ||||
1.3 × 104 | 10/10 | 100 | 7.8 | <1.3 × 104 |
1.3 × 103 | 0/10 | 0 | -- | -- |
1.3 × 102 | 0/10 | 0 | -- | -- |
13 | 0/10 | 0 | -- | -- |
1.3 | 0/10 | 0 | -- | -- |
CDV
R
1A
c
| ||||
Stock 1.2 × 104 | 0/10 | 0 | -- | >1.2 × 104 |
1.2 × 103 | 0/10 | 0 | -- | -- |
1.2 × 102 | 0/10 | 0 | -- | -- |
12 | 0/10 | 0 | -- | -- |
1.2 | 0/10 | 0 | -- | -- |
Mortality | ||||
---|---|---|---|---|
Virusa | Number | Percent | MDDb | LD90 |
VV-WR
c
| ||||
1.6 × 104 | 15/15 | 100 | 8.5 | 4.4 × 103 |
1.6 × 103 | 2/15 | 13 | 8.5 | -- |
1.6 × 102 | 0/15 | 0 | -- | -- |
16 | 0/15 | 0 | -- | -- |
1.6 | 0/15 | 0 | -- | -- |
CDV
R
15A
c
| ||||
Stock, 6 × 104 | 0/15 | 0 | -- | -- |
6 × 103 | 0/15 | 0 | -- | -- |
6 × 102 | 0/15 | 0 | -- | -- |
60 | 0/15 | 0 | -- | -- |
6 | 0/15 | 0 | -- | -- |
CDV
R
16A
c
| ||||
Stock, 8 × 105 | 3/15 | 20 | 8.0 | >8 × 105 |
8 × 103 | 0/15 | 0 | -- | -- |
8 × 102 | 0/15 | 0 | -- | -- |
80 | 0/15 | 0 | -- | -- |
8 | 0/15 | 0 | -- | -- |