Background
Materials and methods
Cell lines and tissue samples
VEGF-C expression | |||||
---|---|---|---|---|---|
case | mean | ± sd | p-value | ||
age | ≧65 | 44 | -0.074 | ± 0.6 | 0.73 |
< 65 | 62 | 0.16 | ± 0.66 | ||
gender | male | 87 | 0.066 | ± 0.65 | 0.06 |
female | 19 | 0.037 | ± 0.63 | ||
Tfactor | Tis | 5 | -0.021 | ± 0.14 | |
T1 | 12 | 0.11 | ± 0.34 | ||
T2 | 11 | -0.098 | ± 0.42 | ||
T3 | 33 | -0.038 | ± 0.7 | ||
T4 | 17 | 0.218 | ± 1.0 | ||
Tis, T1 vs T2-T4 | 0.8 | ||||
Nfactor | N0 | 29 | -0.049 | ± 0.37 | |
N1 | 77 | 0.1 | ± 0.72 | 0.28 | |
Stage | Stage0 | 6 | -0.23 | ± 0.14 | |
Stage1 | 6 | -0.072 | ± 0.35 | ||
Stage2A | 13 | -0.09 | ± 0.31 | ||
Stage2B | 17 | 0.061 | ± 0.47 | ||
Stage3 | 30 | 0.085 | ± 0.66 | ||
Stage4 | 11 | -0.19 | ± 1 | ||
Stage4A | 23 | 0.34 | ± 0.73 | ||
Stage0-2A vs Stage2B-4A | 0.049 | ||||
Histrogical Type | |||||
well | 41 | 0.092 | ± 0.57 | ||
moderate | 56 | 0.053 | ± 0.75 | ||
poor | 9 | -0.087 | ± 0.19 | ||
well vs moderate · poor | 0.34 | ||||
lymphatic invasion | |||||
positive | 69 | 0.056 | ± 0.72 | 0.61 | |
negative | 37 | 0.07 | ± 0.47 | ||
vein invasion | |||||
positive | 54 | 0.024 | ± 0.78 | 0.22 | |
negative | 52 | 0.098 | ± 0.47 |
RNA extraction and RT-PCR analysis
TaqMan gene expression assay
Statistical analysis
Results
Quantitative RT-PCR of VEGF-C in cell lines
Quantitative RT-PCR of VEGF-C in clinical specimens
parameter | Riskratio | 95%aCI | p-value |
---|---|---|---|
Primary tumor | |||
Tis, T1 | 1 | 2.11-16.13 | < 0.001 |
T234 | 5.85 | ||
Lymph node metastasis | |||
N0 | 1 | 1.66-6.9 | < 0.001 |
N1 | 3.38 | ||
Lymph Invasion | |||
Negative | 1 | 0.98-3.11 | 0.056 |
Positive | 1.75 | ||
Vein invasion | |||
Negative | 1 | 0.96-2.72 | 0.067 |
Positive | 1.62 | ||
VEGF-C expression | |||
Low expression | 1 | 1.2-3.4 | 0.0085 |
High expression | 2.02 | ||
aCI; confidence interval |
parameter | Riskratio | 95%aCI | p-value |
---|---|---|---|
Primary tumor | |||
Tis, T1 | 1 | 1.62-12.7 | 0.004 |
T234 | 4.52 | ||
Lymph node metastasis | |||
N0 | 1 | 1.14-4.85 | 0.02 |
N1 | 2.36 | ||
VEGF-C expression | |||
Low expression | 1 | 0.97-2.78 | 0.065 |
High expression | 1.64 | ||
aCI; confidence interval |
VEGF-C expression | |||||
---|---|---|---|---|---|
case | mean | ± sd | p-value | ||
age | ≧65 | 8 | -0.11 | ± 0.34 | 0.15 |
< 65 | 13 | 0.12 | ± 0.33 | ||
gender | male | 19 | 0.06 | ± 0.35 | 0.28 |
female | 2 | 0.25 | ± 0.24 | ||
Tfactor | Tis | 6 | -0.02 | ± 0.14 | 0.029 |
T1 | 15 | 0.13 | ± 0.35 | ||
Nfactor | N0 | 12 | -0.15 | ± 0.27 | |
N1 | 9 | 0.27 | ± 0.3 | 0.003 | |
Stage | Stage0 | 6 | -0.23 | ± 0.14 | |
Stage1 | 6 | -0.072 | ± 0.35 | ||
Stage2A | 1 | -0.09 | |||
Stage2B | 8 | 0.31 | ± 0.29 | ||
Stage0,1 vs Stage2A,2B | 0.014 | ||||
Histrogical Type | |||||
well | 4 | 0.45 | ± 0.18 | ||
moderate | 14 | -0.1 | ± 0.29 | ||
poor | 3 | 0.092 | ± 0.36 | ||
well · moderate vs poor | 0.69 | ||||
lymphatic invasion | |||||
positive | 7 | 0.006 | ± 0.39 | ||
negative | 14 | -0.04 | ± 0.34 | 0.77 | |
vein invasion | |||||
positive | 3 | 0.053 | ± 0.51 | ||
negative | 18 | 0.025 | ± 0.33 | > 0.99 |