Introduction
Materials and methods
Screening of candidate targeted circRNAs
Construction initial circRNA-miRNA-mRNA network
Functional annotation for genes of interest and PPI network analysis
Screening of hub genes and visualizing the miRNA binding sites of the circRNAs
Reconstruction of the circRNA-miRNA-hubgene network
Identification and validation of hubgenes
Results
Construction of weighted gene co-expression modules
Identification DE-circRNA from GEO database and WGCNA co-expression module
Preliminary circRNA-miRNA-mRNA network
circRNA | Chromosome | Start position | End position | Strand | Gene symbol |
---|---|---|---|---|---|
has_circ_0000301 | Chr11 | 47,379,617 | 47,379,952 | – | SPL1 |
MiRNA | Sites |
---|---|
has-miR-1178-3p | 6 |
hsa-miR-1228-3p | 6 |
hsa-miR-377-3p | 11 |
hsa-miR-767-3p | 3 |
Functional annotation for genes of interest
Reconstruction of the circRNA-miRNA-mRNA network
Regulatory axis | circRNA | miRNA | Hubgene |
---|---|---|---|
1 | hsa_circ_0000301 | hsa-miR-1228-3p | CXCL16 |
2 | hsa_circ_0000301 | hsa-miR-1228-3p | IRF4 |
3 | hsa_circ_0000301 | hsa-miR-767-3p | OAS3 |
4 | hsa_circ_0000301 | hsa-miR-767-3p | PTGER3 |