Background
Lung cancer is the leading cause of cancer-related mortality in both men and women worldwide [
1]. More than 80% of lung cancer are non-small-cell lung cancer (NSCLC), which is comprised mostly of squamous cancers and adenocarcinomas [
2,
3]. According to survey of the American Cancer Society (ACS), lung cancer accounts for 28% of all male cancer deaths and 26% of all female cancer deaths in 2014 [
4]. As lung cancer cells infiltrate into surrounding tissue and metastasize to distant organs, the 5-year survival rate in lung cancer patients was very low. In spite of improvements in surgical, radiotherapy, platinum-based doublet chemotherapy and recently developed targeted therapies [
5], the prognosis of NSCLC is still very poor and about 30–55% of the patients who are diagnosed early and treated by surgery will develop a recurrence [
6,
7]. The targeted therapies interrupt signaling pathways which responsible for lung cancer cell proliferation and survival [
5]. Hence, it is urgent to understand the potential molecular mechanisms mediating the NSCLC tumorigenesis and identify novel biomarkers to help provide individualization treatment and assess better prognosis [
8,
9].
According to the NCBI database (
http://www.ncbi.nlm.nih.gov/), Coiled-coil-helix-coiled-coil-helix domain-containing protein 2 (CHCHD2) also named Mitochondria Nuclear Retrograde Regulator 1 (MNRR1) was located on human chromosome 7p11.2, containing 4 exons, 456 nucleotide bases and encoding 151 amino acids. It was identified as a member of the protein family which containing a coiled-coil-helix-coiled-coil-helix (CHCH) domain. There was a single putative that CHCHD2 consists of an N-terminal mitochondrion localization sequence and a strongly conserved C-terminal CHCH domain, and they were together performed their function in the cells [
10]. CHCHD2 was a gene that has not yet been clearly defined, and previous research revealed that CHCHD2 played a role in translation in human cells [
11]. Furthermore the CHCHD2 cDNA was found to promote cell migration and altered cell adhesion when ectopic overexpressed in NIH3T3 fibroblasts [
10]. Subsequently, CHCHD2 was found highly expressed in the HCC specimens [
12] and co-amplified with EGFR in NSCLC [
13], however, the expression of CHCHD2 in NSCLC and how will that affect the progress and prognosis of the NSCLC was not confirmed. To study the clinicopathologic features and prognostic implications of CHCHD2 expression in patients with NSCLC, we investigated the expressions of CHCHD2 in NSCLC by immunohistochemical staining, Real Time quantitative PCR and Western blot, and assessed the relationships between CHCHD2 and clinical parameters.
In computational screen, CHCHD2 was identified as a protein necessary for OxPhos function [
14]. Further research demonstrated that under reduced oxygen tension microenvironment, CHCHD2 expression was enhanced [
15]. Hypoxia was typically associated with many types of solid tumors [
16], Hypoxia-inducible factor-1 (HIF-1) was a principle modulator of the tumor cell response to hypoxia, and HIF-1α level was normally kept low by proteasomal degradation, it rapidly stabilized under conditions of hypoxia [
17]. Given all these elements, we speculated that CHCHD2 might was a novel molecular to regulator HIF-1α function and hypoxia signaling. Thus, the present study also focused on the correlation of CHCHD2 and HIF-1α expression in NSCLC, suggesting that CHCHD2 might interacted with HIF-1α to response hypoxia in the occurrence of NSCLC, in order to provide a basis for further study on the developmental mechanism of CHCHD2 in regards to NSCLC.
Methods
Patients and tissue samples
A total of 209 NSCLC patients tissue specimens were collected from 2006 to 2011, admitted by Department of Thoracic Surgery of Tangdu Hospital. All of these enrolled patients did not receive chemotherapy or targeted therapy. The detailed clinicopathologic features information of the patients were obtained from the medical records in a computerized registry database were summarized in Table
2. The patients median age was 62 years (range, 31–81 years). The operation date is considered as the starting date for estimating the postoperative survival time, and the deadline for follow-up was 30 July, 2016. The median follow-up period of these patients was 36 months (range, 0–79 months). The research protocol was approved by the Regional Ethics Committee for Clinical Research of the Air Force Medical University. All patients provided written informed consent and agree to use their medical records and tissue samples for research purposes.
Immunohistochemistry
The expression of CHCHD2 and HIF-1α protein in all the tissue sections of the subjects were investigated by rabbit antibodies (anti-CHCHD2, diluted 1:50, ABGENT, SanDiego, CA or anti-HIF-1α, diluted 1:100, Proteintech™, Chicago, USA). The detailed operation process and staining score of immunohistochemistry accordance with our previous methods [
18]. The final immunohistochemical staining score reported is the average of the scores from the tow investigators.
RNA isolation and quantitative RT- PCR
Extraction of total RNA from frozen tissues was performed using TRIzol reagent (Invitrogen, USA). RevertAid First-Strand cDNA Synthesis Kit (Thermoscientific, Vilnius, Lithuania) was used to prepare reverse transcription according to the manufacturer’s protocols. The qRT-PCR reaction was carried out using LightCycler-Fast-Start DNA Master SYBR Green (Roche Diagnostics, Tokyo, Japan). Gene expression was normalized to β-actin. Three parallel reactions were set for each sample. The mRNA expression value were calculated by the MxPro QPCR Software 4.10 (Mx3005P, Stratagene, Agilent Technologies) according to 2-△△CT method. The primers used for qRT-PCR were: CHCHD2, F-5′- CAG TTG GCT CTTBCTGBCTG CT-3′ and R-5′-GTA ATG GCG TGA CCC AAT GT-3′; HIF-1α, F-5′-TGC AAC ATG GAA GGT ATT GC − 3′ and R-5′-TTC ACA AAT CAG CAC CAA GC − 3′; β-actin, F-5′-TCC CTG GAG AAG AGC TAC GA-3′ and R-5′-AGC ACT GTG TTG GCG TAC AG-3′.
Western blot
The frozen Tissue was cut into pieces and placed in RIPA Lysis Buffer (P0013B, Beyotime Biotechnology) containing protease inhibitor (Phenylmethanesulfonyl fluoride, PMSF, ST506, Beyotime Biotechnology) on ice for 1 h. Then the mixture was homogenized, centrifuged at 12,000 rpm for 20 min at 4 °C. The supernatant was collected and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Total proteins were separated by electrophoresis using SDS-PAGE gel and transferred onto PVDF membrane. After by blocking in 9% not-fat milk, the membrane was incubated with specific antibodies overnight at 4 °C (anti-CHCHD2, 1: 500; anti-HIF-1α 1:300; anti-β-actin, 1: 1000). Then washing with TBST for 30 min, the membrane was incubated with a Rabbit anti-Human secondary antibody (1:5000) for 1 h at temperature. For each membrane, band intensity was analyzed using the Millipore chromogenic kit (Millipore, Billerica, MA, USA) and quantitatively analyzed using Quantity software (Bio-Rad, USA).
Immunohistofluorescence
According to immunohistochemistry procedures, the slides were incubated with primary antibodies (anti-CHCHD2, 1:50) and stained with goat anti-rabbit (Alexa Fluor 488; Zhuangzhi Bio, Xi′an, China), washed with PBS. Then the slides were incubated with anti-HIF-1α (diluted 1:100) antibodies and stained with goat anti-rabbitat (Cy3; Zhuangzhi Bio, Xi′an, China). After the final washing, the slides were mounted in 50% glycerol (in PBS) and examined by a fluorescence microscope (Leica DM4000B, Leica, Wetzlar, Germany).
Oncomine analysis
In order to further explain the expression level of CHCHD2 in NSCLC and its prognostic value, we used Oncomine database (
https://www.oncomine.org/) to analyze. Search the target gene CHCHD2, and filter as follows, Analysis Type: Lung Cancer vs. Normal Analysis, Sample Type: Surgical Specimen. Select the reporter (217720_at) for meta-analysis. The sample names, tissue types, and expression values (log2 median-centered intensity) of the included data sets were recorded, and the mRNA expression level of CHCHD2 was statistically analyzed using Graphpad Prism 5 software. Search the target gene CHCHD2, and filter as follows, Cancer Type: Lung Cancer, Clinical Outcome: Survival Status, Sample Type: Surgical Specimen. Record the Sample name, Tissue type and Expression value, Survival time, Survival status. The median of gene expression value as cut off value, and gene expression was divided into low and high expression. Survival analysis with Graphpad prism 5.
Statistical analysis
Statistical analyses were done with SPSS17.0 Software (SPSS Inc., Chicago, IL, USA). The Wilcoxon (W) text was used to evaluate the comparison of CHCHD2 and HIF-1α protein expression between NSCLC and corresponding normal tissue. Associations between immunohistochemical expression and clinical variables were evaluated by Mann-Whitney U test (among tow groups), Kruskal-Wallis H test (among multiple groups) and Spearman’s rank correlation analysis as appropriate. rs>0.3, P<0.05 was considered to be statistically significant. The Cox proportional hazards model was used for univariate and multivariate analyses. Survival curves were examined using the Kaplan-Meier method, and compared using the log-rank test of GraphPad Prism software version 5 (GraphPad Software, Inc., CA, USA).
Discussion
CHCHD2 is well-conserved among different species from humans to yeast, mouse and human CHCHD2 share 87% amino acid sequence identity [
22]. Previous studies have found that CHCHD2 contains CHCH domain, while the proteins which contain the CHCH domain have diverse functions [
23,
24]. Some studies have shown that the expression of CHCHD2 are related to glycolysis and translation [
11,
12] or play an importent role in enhancing cell migration-promoting activity [
12,
25]. However, the CHCHD2 expression on the prognosis of NSCLC has not been reported. The purpose of this study was to investigate the clinical significance of the expression of CHCHD2 in NSCLC and to reveal the possible mechanism of the expression CHCHD2 for the adverse prognosis of NSCLC.
Previously, human
CHCHD2 gene was determined overexpression in some of cancer tissues [
1,
2]. In our study, we firstly provided evidence that CHCHD2 mRNA and protein overexpressed in NSCLC tissues by qRT-PCR and Western blot methods. Then based on the data of oncomine database, it was confirmed that the mRNA expression level of CHCHD2 in NSCLC was higher than that in normal tissue. Further more we detected the protein expression of CHCHD2 in 209 pairs of specimens by immunohistochemistry, the positive rate of CHCHD2 (94.7%) with NSCLC tissues was higher than that of normal tissues (11.0%) (
P = 0.000). Consequently, both previous and our studies all showed that CHCHD2 overexpressed in cancerous tissues. These data suggest that CHCHD2 may be a new biomarker for lung cancer.
Subsequently, we found that the expression of CHCHD2 in NSCLC was notably associated with some clinical parameters of NSCLC, such as smoking (
P = 0.045), tumor size (
P = 0.000), differentiation degree (
P = 0.034), TNM stage (
P = 0.000), lymph metastasis (
P = 0.000). These findings indicate that CHCHD2 may play an import role in the proliferation and metastasis of NSCLC. And in the report of Minchul Seo [
10], overexpression of CHCHD2 can promote the migration of NIH3T3 fibroblasts cells, and knockdown of the endogenous CHCHD2 can reduce the motility of NIH3T3 fibroblasts cells. Combined with our results and existing reports, it is suggested that CHCHD2 may be related to the proliferation, invasion and metastasis of NSCLC.
In addition, we analyzed the relationship between tumor size, differentiation, TNM stage, lymph node metastasis and patient survival, these factors were all related to CHCHD2 expression. The results showed that patients with tumor size > 5 cm, poor differentiation, high TNM stage and lymph node metastasis had shorter median survival time and lower 5-year survival rates. These evidences indicated that tumor size, differentiation, TNM stage, lymph node metastasis would been important factors of surgical patients’ survival rates. Then we analyzed the relationship between the expression of CHCHD2 and HIF-1a and survival rates of patients. These results indicated that patients with high expression of CHCHD2 and HIF-1a had shorter median survival time and lower 5-year survival rates, which suggested that they were also important factors affected the survival rates of surgical patients. Therefore, CHCHD2 would been an adverse prognostic factor of NSCLC.
To further clarified CHCHD2 expression was a adverse prognostic factor of NSCLC, a Cox’s proportional hazards model was used for regression analysis. According to the results of univariate analysis, tumor size, grade of differentiation, TNM stage and lymph node metastasis showed significantly higher hazard ratios for adverse prognostic. Multivariate analysis showed that grade of differentiation, lymph node metastasis CHCHD2 and HIF-1α expressions were all showed significantly higher hazard ratios, which indicated that these factors would been independent prognostic factors of NSCLC. Thus, these evidences once again proved that CHCHD2 expression was a independent adverse prognostic factor of NSCLC. Due to the limitations of the sample size and follow-up, the median survival time in many groups could not be obtained. To compensate for these shortcomings, we intend to carry out further multicenter clinical studies, expand the sample size and enrich the means of detection [
26].
Meanwhile, we found that HIF-1α was overexpression in NSCLC tissues and had a poor prognosis. Moreover, we found an interest problem that when CHCHD2 expressed in the nucleus, HIF-1α also expressed in the nucleus. It suggested that maybe CHCHD2 and HIF-1α are co-expressed in the nucleus of cells in NSCLC. Therefore we investigate the correlation of CHCHD2 with HIF-1α for the first. The correlation of CHCHD2 with HIF-1α was significant in regards to histological type, differentiation and lymph node metastasis (P<0.01, rs >0.3). In order to further verify whether there was a co-localization phenomenon for the CHCHD2 and HIF-1α expression, immunofluorescence experiments were conducted. The results showed that the co-localization phenomenon existed. Thus, we suspect that if the expression of CHCHD2 accompanies the HIF-1α expression participated in the regulation signaling pathways, so that lung cancer cells could adapt to hypoxic environment and play a role in angiogenesis, invasion, metastasis and metabolism of lung cancer.
Conclusions
This study showed that the expression of CHCHD2 and HIF-1α were higher in NSCLC tissues than normal tissues, and the expressions of these proteins were significantly associated with differentiation, TNM stage and lymphatic metastasis. Moreover, that high CHCHD2 and HIF-1α expression are associated with poor prognosis in NSCLC patients. All these signs indicated that CHCHD2 may play an important role in migration, invasion and metastasis of NSCLC. Present study indicate a potential role for CHCHD2 expression as an independent predictive factor of poor prognosis in NSCLC patients, and put forward the expressions of CHCHD2 maybe accompanies the HIF-1α expression, which will activate the hypoxic genes in this pathway. However, further study with larger sample size and cytological experiments are needed to confirm these results, and the specific pathway and mechanism driving this effect are also need to be further study.
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