Background
Methods
Study design and patients
Histological analysis
DNA analysis by pyrosequencing
Bioinformatics analysis and metagenomic prediction
Results
Sample_code | gender | age | Histology | Maximum diameter | Regiona | Root canal therapyb |
---|---|---|---|---|---|---|
RC_1 | M | 28 | RC | 21 mm | 4.6 | + 4.6 |
RC_2 | F | 30 | RC | 20 mm | 3.6 | – |
RC_3 | M | 31 | RC | 24 mm | 4.6 | + 4.6 |
RC_4 | M | 29 | RC | 22 mm | 1.1, 1.2, 1.3 | + 1.1 |
RC_5 | M | 40 | RC | 13.5 mm | 4.6 | – |
PG_6 | F | 23 | PG | 20 mm | 1.6, 1.7 1.8 | + 1.6, 1.7, 1.8 |
PG_7 | F | 39 | PG | 20 mm | 3.6 | – |
PG_8 | F | 25 | PG | 15 mm | 4.7 | + 4.7 |
PG_9 | M | 36 | PG | 20 mm | 2.6, 2.7 | + 2.6, 2.7 |
PG_10 | F | 20 | PG | 11 mm | 3.8 | – |
Microbial diversity
Samplea | OTUs | ESC | chao1 | Shannon Index |
---|---|---|---|---|
RC_1 | 202.00 | 1.00 | 209.46 | 4.48 |
RC _2 | 67.00 | 0.99 | 80.13 | 2.21 |
RC _3 | 164.00 | 1.00 | 171.16 | 2.24 |
RC _4 | 86.00 | 0.99 | 109.40 | 2.86 |
RC _5 | 143.00 | 1.00 | 155.83 | 2.34 |
PG_6 | 129.00 | 1.00 | 135.00 | 2.58 |
PG_7 | 49.00 | 0.99 | 56.33 | 1.90 |
PG_9 | 22.00 | 0.96 | 61.00 | 2.29 |
PG_10 | 49.00 | 0.97 | 76.60 | 2.34 |
OTUs | RCs | SD | Type | OTUs | PGs | SD | Type |
---|---|---|---|---|---|---|---|
Lactococcus lactis
| 54.20 | 12.73 | Facultative anaerobe |
Lactococcus lactis
| 59.63 | 9.97 | Facultative anaerobe |
Propionibacterium acnes
| 17.96 | 8.37 | Facultative anaerobe |
Propionibacterium acnes
| 18.68 | 7.84 | Facultative anaerobe |
Corynebacterium matruchotii
| 5.51 | 4.76 | Facultative anaerobe |
Corynebacterium matruchotii
| 5.83 | 4.96 | Facultative anaerobe |
Staphylococcus warneri
| 4.27 | 1.13 | Facultative anaerobe |
Staphylococcus warneri
| 5.67 | 7.32 | Facultative anaerobe |
Gemellales
| 2.75 | 2.83 | Anaerobe |
Lactobacillus zeae
| 2.59 | 3.52 | Facultative anaerobe |
Actinomyces johnsonii
| 2.31 | 4.52 | Facultative anaerobe |
Gemellales
| 1.06 | 0.11 | Anaerobe |
Lactobacillus zeae
| 1.63 | 1.92 | Facultative anaerobe |
Pseudomonas
| 0.40 | 0.23 | Aerobic |
Capnocytophaga ochracea
| 1.04 | 2.00 | Facultative anaerobe |
Streptococcus mitis
| 0.35 | 0.22 | Facultative anaerobe |
Streptococcus mitis
| 0.81 | 0.68 | Facultative anaerobe |
Paracoccus
| 0.31 | 0.26 | Anaerobe |
Paracoccus
| 0.68 | 0.61 | Anaerobe |
Finegoldia
| 0.24 | 0.22 | Anaerobe |
Pseudomonas
| 0.51 | 0.17 | Aerobic |
Sphingomonas
| 0.23 | 0.07 | Aerobic |
Fusobacterium nucleatum
| 0.50 | 0.43 | Anaerobe |
Sediminibacterium
| 0.18 | 0.24 | Facultative anaerobe |
Leptotrichia
| 0.39 | 0.65 | Anaerobe |
Anaerococcus
| 0.15 | 0.14 | Anaerobe |
Prevotella intermedia
| 0.26 | 0.28 | Anaerobe |
Enhydrobacter
| 0.15 | 0.18 | Anaerobe |
Finegoldia
| 0.23 | 0.17 | Anaerobe |
Streptococcus
| 0.14 | 0.13 | Facultative anaerobe |
Streptococcus
| 0.23 | 0.10 | Facultative anaerobe |
Acinetobacter lwoffii
| 0.10 | 0.12 | Aerobic |
Bradyrhizobium
| 0.19 | 0.06 | Aerobic |
Methylobacterium
| 0.10 | 0.13 | Anaerobe |
Micrococcus luteus
| 0.19 | 0.19 | Aerobic |
Actinomyces johnsonii
| 0.09 | 0.11 | Facultative anaerobe |
Enhydrobacter
| 0.18 | 0.09 | Anaerobe |
Micrococcus luteus
| 0.09 | 0.13 | Aerobic |
Rothia dentocariosa
| 0.18 | 0.38 | Anaerobe |
Bradyrhizobium
| 0.08 | 0.07 | Aerobic |
Anaerococcus
| 0.17 | 0.22 | Anaerobe |
Rothia dentocariosa
| 0.08 | 0.15 | Anaerobe |
Acinetobacter lwoffii
| 0.16 | 0.13 | Aerobic |
Fusobacterium nucleatum
| 0.07 | 0.10 | Anaerobe |
Methylobacterium
| 0.15 | 0.08 | Anaerobe |
Acinetobacter johnsonii
| 0.06 | 0.10 | Aerobic |
Campylobacter
| 0.12 | 0.16 | Facultative anaerobe |
Leptotrichia
| 0.03 | 0.03 | Anaerobe |
Sediminibacterium
| 0.11 | 0.08 | Facultative anaerobe |
Capnocytophaga ochracea
| 0.01 | 0.02 | Facultative anaerobe |
Acinetobacter johnsonii
| 0.10 | 0.08 | Aerobic |
Prevotella intermedia
| 0.01 | 0.03 | Anaerobe |
Sphingomonas
| 0.08 | 0.08 | Aerobic |
Campylobacter
| 0.00 | 0.00 | Facultative anaerobe |