Background
Methods
Chemical and reagents
Plant materials
Animals
Acute toxicity assessment
Body weight and liver weight variation analysis
Water and food assessment
Effect of poncirin on coordination and motor functions
Survival rate
NO determination
Hematological and serum analysis
Histopathological study of liver tissues
Effect of poncirin on liver anti-oxidants and oxidative stress markers
Effect of the poncirin on myeloperoxidase (MPO) activity
Evans blue vascular permeability
Measurement of IL-1β, IL-6 and TNF-α production
Molecular docking study
Statistical analysis
Results
Effect of the poncirin on the acute toxicity
Effect of poncirin treatment on the body weight and liver weight
Effect of poncirin treatment on water and food assessment following CCL4-induced liver injury
Effect of poncirin treatment on the CCL4-induced muscle strength and coordination
Survival analysis
Effect of poncirin treatment on the blood chemistry
Parameters | WBC (109/L) | LYM (109/L) | NEU (109/L) | MON (109/L) | EOS (109/L) | PLT (109/L) | RBC (1012/L) | HB (g/dL) |
---|---|---|---|---|---|---|---|---|
Normal control | 3.91 ± 0.30 | 4.2 ± 0.90 | 7.1 ± 0.04 | 0.4 ± 0.10 | 0.21 ± 0.05 | 213.0 ± 15.01 | 13.1 ± 22 | 15.3 ± 0.34 |
CCl4 Treated | 7.01 ± 0.32### | 6.3 ± 0.30### | 11.2 ± 0.3### | 0.91 ± 0.30### | 0.40 ± 0.01### | 423.7 ± 9.0### | 8.5 ± 13.6### | 11.5 ± 1.0### |
Silymarin 50 mg/kg | 4.56 ± 0.11** | 4.7 ± 0.21* | 7.6 ± 0.21* | 0.53 ± 0.20* | 0.24 ± 0.20** | 278.2 ± 3.2** | 12.3 ± 25* | 14.0 ± 0.2* |
Poncirin 30 mg/kg | 4.89 ± 0.23** | 4.8 ± 0.02* | 8.2 ± 0.09* | 0.50 ± 0.12* | 0.27 ± 0.02** | 256.0 ± 87** | 12.0 ± 21* | 14.3 ± 0.45* |
Effect of poncirin on biochemical parameters and serum enzymes
Sample | ALT (μl/dl) | AST (μl/dl) | ALP (μ/dl) | Total Bilirubin (mg/dl) | Total Protein (g/dl) |
---|---|---|---|---|---|
Normal control | 43.12 ± 2.5 | 40.03 ± 0.7 | 37.04 ± 1.8 | 0.24 ± .03 | 8.18 ± 0.36 |
CCL4 treated | 215.1 ± 1.3### | 198.1 ± 3.0### | 66.3 ± 2.6### | 1.04 ± 0.06### | 4.44 ± 0.46### |
Silymarin 50 mg/kg | 78.8 ± 4.24** | 84.5 ± 5.65* | 40.23 ± 1.4** | 0.375 ± 0.10** | 7.52 ± 0.14* |
Poncirin 30 mg/kg | 80.3 ± 4.04** | 95.6 ± 8.14* | 40.65 ± 2.8** | 0.38 ± 0.22** | 7.89 ± 0.31* |
Effect of the poncirin treatment on the CCL4-induced electrolytes imbalance
Sample | Sodium (mEq/L) | Potassium (mEq/L) | Bicarbonate (mEq/L) |
---|---|---|---|
Normal control | 132.1 ± 0.01 | 9.5 ± 0.7 | 27.5 ± 0.4 |
CCL4 treated | 111.6 ± 0.70### | 10.3 ± 0.56### | 38.4 ± 1.7### |
Silymarin 50 mg/kg | 124.03 ± 0.3*** | 9.3 ± 0.78 | 31.09 ± 0.06** |
Poncirin 30 mg/kg | 120.24 ± 0.50*** | 8.43 ± 0.30 | 32.9 ± 0.09* |