Introduction
Materials and methods
Microarray data
Dataset | Control | IPF | Total number |
---|---|---|---|
GSE10667 | 15 | 23 | 38 |
GSE110147 | 11 | 22 | 33 |
GSE71351 | 4 | 8 | 12 |
Identification of differentially expressed genes
GO and KEGG function enrichment analyses
PPI network and hub genes identification
Molecular docking
Western blot testing
Statistical analysis
Results
Identification of DEGs
GO and KEGG enrichment analysis
Biological functions | Term | Count | P |
---|---|---|---|
Cellular component | Plasma membrane | 35 | 0.023 |
Cellular component | An integral component of the membrane | 35 | 0.029 |
Cellular component | Extracellular region | 22 | 0.000 |
Cellular component | Extracellular space | 18 | 0.008 |
Cellular component | Extracellular exosome | 18 | 0.027 |
Cellular component | Golgi apparatus | 11 | 0.040 |
Transcriptional misregulation in cancer | Hsa05202 | 5 | 0.042 |
Calcium signaling pathway | Hsa04020 | 6 | 0.023 |
p53 signaling pathway | Hsa04115 | 4 | 0.013 |
FoxO signaling pathway | Hsa04068 | 5 | 0.012 |
Mineral absorption | Hsa04978 | 4 | 0.008 |
Melanoma | Hsa05218 | 5 | 0.001 |
Proteoglycans in cancer | Hsa05205 | 5 | 0.048 |
PPI network and hub genes
Gene names | Gene descriptions | Scores |
---|---|---|
CTGF | Connective tissue growth factor | 20 |
COL5A2 | Collagen type V alpha 2 chain | 20 |
LOX | Lysyl oxidase | 19 |
COL3A1 | Collagen type III alpha 1 chain | 16 |
FBN1 | Fibrillin 1 | 14 |
HGF | Hepatocyte growth factor | 12 |
FBN2 | Fibrillin 2 | 12 |
HBEGF | Heparin-binding EGF-like growth factor | 10 |
FGF7 | Fibroblast growth factor 7 | 10 |
ITGAV | Integrin subunit alpha V | 10 |