Background
Cervical cancer is the second most common cancer in female, next to breast cancer, accounting for approximately 527,000 new cancer cases and 265,000 cancer deaths each year worldwide [
1]. With great development of diagnostic and therapeutic strategies, including cervical cancer early screening, surgical resection, chemotherapy, and radiotherapy, the 5-year survival rate of cervical cancer has reached to 60–70% [
2]. However, considerable cervical cancer patients’ long-term survivals are dismal [
3]. Therefore, it is urgent to investigate the molecular mechanisms underpinning the initiation and progression of cervical cancer and develop better therapeutic modalities for cervical cancer therapy.
Accumulating evidences revealed that many non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are frequently deregulated and have critical roles in multiple cancers [
4‐
10]. These non-coding RNAs could be novel candidates for prognostic biomarkers and therapeutic targets in various cancers including cervical cancer [
9‐
13]. LncRNAs are a class of novel non-coding transcripts with more than 200 nucleotides in length [
14,
15]. Next-generation sequencing technologies have identified more than 58,000 lncRNAs among human transcriptome, while the number of protein-coding genes is only 21,000 [
16]. A variety of lncRNAs are revealed to be deregulated and associated with patients’ prognosis in several cancers including cervical cancer [
17‐
20]. Furthermore, many lncRNAs are shown to regulate various biological aspects of cancer cells, such as cell proliferation, apoptosis, cell cycle, migration, invasion, drug-resistance, and so on [
21‐
23]. These lncRNAs may be implicated in many signaling pathways critical for cancers, and regulate various oncogenes and tumor suppressors in different cancers [
24‐
27].
LncRNA differentiation antagonizing non-protein coding RNA (DANCR), previously termed as ANCR, was first revealed to suppress progenitor differentiation [
28]. Subsequent studies demonstrated that DANCR is a promising cancer-associated lncRNA [
29]. DANCR is shown to be upregulated in gastric cancer, lung cancer, glioma, colorectal cancer, retinoblastoma, osteosarcoma, oesophageal cancer, breast cancer, prostate cancer, and hepatocellular carcinoma [
30‐
37]. In these different cancers, DANCR mainly functions as an oncogene via promoting cell proliferation, invasion, migration, and/or inhibiting cell apoptosis [
30‐
37]. However, the mechanisms underpinning the functions of DANCR in different cancers are various. DANCR was reported to modulate PI3K-Akt pathway in osteosarcoma, β-catenin pathway in hepatocellular carcinoma, miR-634-RAB1A signaling pathway in glioma, androgen-AR signaling pathway in prostate cancer [
31,
32,
38,
39]. In cervical cancer, however, the expressions, roles, and mechanisms of action of DANCR are still unclear.
In the present study, we determined DANCR expression in cervical cancer tissues and cell lines, analyzed the correlation between DANCR expression and cervical cancer patients’ clinicopathological features, including prognosis. Furthermore, gain-of-function and loss-of-function assays were performed to investigate the biological roles of DANCR in cervical cancer growth. Finally, using public available dataset, combined with experimental verification, we investigated the mechanisms underlying the biological effects of DANCR in cervical cancer. We demonstrated that DANCR, which is an oncogenic lncRNA in cervical cancer through activating the Wnt/β-catenin signaling pathway, might be a promising prognostic biomarker and therapeutic target for cervical cancer.
Methods
Patient tissue samples
A total of 82 pairs of cervical cancer and adjacent noncancerous cervix tissues were obtained from cervical cancer patients with informed written consent who underwent potentially curative surgery in the First Affiliated Hospital of Zhengzhou University (Zhengzhou, China) from 2014 to 2016. All tissue samples were diagnosed by histopathological examination. Freshly resected tissue specimens were immediately frozen in liquid nitrogen and stored at − 80 ℃ until use. The Medical Ethics Committee of the First Affiliated Hospital of Zhengzhou University reviewed and approved this program in accordance with Helsinki Declaration.
Cell culture and treatment
The human normal cervical epithelial cell line HCerEpiC was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). The cervical cancer cell lines HeLa, SiHa, C-33A, and ME-180 were purchased from American Type Culture Collection (Rockville, MD, USA). HCerEpiC cells were grown in Cervical Epithelial Cell Growth Supplement (ScienCell, Carlsbad, CA, USA). HeLa, SiHa, and C-33A cells were grown in Eagle’s Minimum Essential Medium (Invitrogen Carlsbad, CA, USA). ME-180 cells were grown in McCoy’s 5A Medium (Sigma-Aldrich, St. Louis, MO, USA). All media were added with 10% fetal bovine serum (Gibco, Grand Island, NY, USA). Where indicated, the cervical cancer cells were treated with 5 μM ICG-001 (Selleck Chemicals, Houston, TX, USA). All cell lines were grown in a humidified incubator with 5% CO2 at 37 ℃.
Total RNA was extracted from indicated tissues and cells with TRIzol Regent (Invitrogen) following the protocol. After being treated with DNase I (Takara, Dalian, China) to remove genomic DNA, the isolated RNA was subjected to reverse transcription using a PrimeScript RT Reagent Kit (Takara) following the protocol. Next, the cDNA was subjected to quantitative Real-Time PCR (qRT-PCR) using SYBR Premix Ex Taq (Takara) and gene specific primers. Primer sequences are as follows: for DANCR, 5′-GCGCCACTATGTAGCGGGTT-3′ (sense) and 5′-TCAATGGCTTGTGCCTGTAGTT-3′ (antisense); for FRAT1, 5′-TGGAAGCGAGAGTAAAAAGC-3′ (sense) and 5′-GGTCACGCCAAATAAGGAG-3′ (antisense); for FRAT2, 5′-TACCTCACTTAGCCCTTGG-3′ (sense) and 5′-ATGCGTGTCGTTAGTTTTCA-3′ (antisense); for C-myc, 5′-GCTGCTTAGACGCTGGATTT-3′ (sense) and 5′-CTCCTCCTCGTCGCAGTAGA-3′ (antisense); for Cyclin D1, 5′-TTCCTGTCCTACTACCGC-3′ (sense) and 5′-CTCCTCCTCTTCCTCCTC-3′ (antisense); and for β-actin, 5′-GGGAAATCGTGCGTGACATTAAG-3′ (sense) and 5′-TGTGTTGGCGTACAGGTCTTTG-3′ (antisense). The quantification of RNA expression was calculated following the comparative Ct method. β-actin was used as endogenous control.
Plasmids construction and transfection
DANCR expressing plasmid pcDNA3.1-DANCR was constructed as previously described [
40]. Briefly, DANCR full-length sequence was PCR amplified using the PrimeSTAR HS DNA polymerase (Takara) and the primers 5′-CCCAAGCTTGCCCTTGCCCAGAGTCTTC-3′ (sense) and 5′-CGGGATCCGTCAGGCCAAGTAAGTTTATTAAC-3′ (antisense). Next, the PCR products were subcloned into the Hind III and BamH I sites of pcDNA3.1. DANCR knockdown plasmid was constructed as previously described [
38]. Briefly, one pair of cDNA oligonucleotides specifically targeting DANCR was inserted into the shRNA expression plasmid pGPH1/Neo (GenePharma, Shanghai, China). The sequences of DANCR specific shRNA were: 5′-CACCAGCCAACTATCCCTTCAGTTACATTCAAGAGATGTAACTGAAGGGATAGTTGGCTTTTTTTG-3′ (sense) and 5′-GATCCAAAAAAAGCCAACTATCCCTTCAGTTACATCTCTTGAATGTAACTGAAGGGATAGTTGGCT-3′ (antisense). The sequences of control scrambled shRNA were: 5′-CACCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTG-3′ (sense) and 5′-GATCCAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAC-3′ (antisense). Plasmids transfection was carried out using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocols.
Stable cell lines construction
For construction of DANCR stably overexpressed and control HeLa cells, DANCR expressing plasmid pcDNA3.1-DANCR or control empty plasmid pcDNA3.1 was transfected into HeLa cells. After 48 h, the transfected HeLa cells were treated with neomycin for 4 weeks. To construct DANCR stably depleted and control C-33A cells, DANCR specific shRNA or control scrambled shRNA was transfected into C-33A cells. After 48 h, the transfected C-33A cells were treated with neomycin for 4 weeks. The efficiencies of overexpression and knockdown were determined by qRT-PCR.
Cell proliferation assays
Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) and Ethynyl deoxyuridine (EdU) assays. For CCK-8 assay, DANCR stably overexpressed or depleted cervical cancer cells were resuspended and plated into 96-well plates at a density of 3000 cells per-well. After culture for indicated time, CCK-8 solution (Beyotime, Shanghai, China) was added into the wells. After 2 h of continued culture, absorbance values of optical density (OD) at 450 nm for each well were detected using automatic enzyme-linked immune detector. EdU assay was performed with the Cell-Light™ EdU Apollo®643 In Vitro Imaging Kit (RiboBio, Guangzhou, China) according to the instructions. Results were analyzed by Zeiss fluorescence photomicroscope (Carl Zeiss, Oberkochen, Germany) via randomly counting ten fields.
Mouse xenograft model
1 × 10
6 DANCR stably overexpressed or depleted cervical cancer cells were resuspended in 100 μl phosphate buffered saline containing 50% matrigel (Invitrogen) and then subcutaneously injected into the flanks of female BALB/c-nu mice of 6 weeks old. The mice were maintained in a sterile environment on a daily 12-h light/12-h dark cycle. Subcutaneous tumor volumes were measured every 3 days using caliper and calculated following the formula: tumor volumes = 0.5 × length × width
2. At the twenty-first day after injection, the mice were sacrificed and subcutaneous tumors were resected and weighed. The Animal Ethics Committee of the First Affiliated Hospital of Zhengzhou University reviewed and approved this program. Ki67 immunohistochemical staining was carried out as we previously described with Ki67 primary antibody (Cell Signaling Technology, Boston, MA, USA) [
41].
Western blot
Nuclear proteins were extracted from DANCR stably overexpressed or depleted cervical cancer cells using CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich). Total proteins were extracted from indicted cervical cancer cells using RIPA buffer (Beyotime). Next, nuclear proteins or total proteins were subjected to 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). After being transferred onto polyvinylidene fluoride (PVDF) microporous membrane (Millipore, Boston, MA, USA), the blots were incubated with primary antibodies against β-catenin (Abcam, Hong Kong, China), histone H3 (Abcam), FRAT1 (Abcam), FRAT2 (Sigma-Aldrich), C-myc (Cell Signaling Technology), Cyclin D1 (Cell Signaling Technology), or GAPDH (Cell Signaling Technology). Histone H3 was employed as a loading control for nuclear protein, and GAPDH was employed as a loading control for total protein. The blots were visualized with chemiluminescence.
Statistical analysis
All statistical analyses were carried out using the GraphPad Prism 5.0 version (La Jolla, CA, USA). For comparisons, Wilcoxon signed-rank test, Pearson Chi square test, log-rank test, Student’s t-test, Mann–Whitney test, and Pearson correlation analysis were carried put as indicated. Results were considered as statistically significant when p < 0.05.
Discussion
Genomic and molecular features of cervical cancer are complex [
46,
47]. Accumulating studies have identified various genomic mutations of
PIK3CA,
EP300,
FBXW7,
PTEN,
HLA-A,
ARID1A and so on in different cervical cancer tissues [
11,
46]. The aberrant expression of many mRNAs, including FGFR3, CTGF, TP63, IL36G, ADH7, SPINK5, and so on, have also been identified in cervical cancer [
47]. Furthermore, the dysregulation of non-coding RNAs gradually attracts researchers’ attention, such as miR-205, miR-200a, miR-30a, lncRNA BCAR4, lncRNA HOTAIR, lncRNA MALAT1, lncRNA MEG3, and so on [
46,
47]. Due to the huge amount of lncRNAs, the clinical significances of most of lncRNAs in cervical cancer are unclear.
In the present study, we focused on a lncRNA DANCR, which is located on chromosome 4q12. Although DANCR has been investigated in several cancers, and been regarded as a cancer-associated lncRNA [
29], the functions and clinical significances of DNACR in cervical cancer are unclear. In this study, we identified DANCR is upregulated in cervical cancer tissues and cell lines compared with adjacent noncancerous cervix tissues and normal cervical epithelial cell line, respectively. High expression of DANCR is positively associated with large tumor size, advanced FIGO stage, and poor overall survival of cervical cancer patients. Functional experiments demonstrated that ectopic expression of DANCR promotes cervical cancer cell proliferation in vitro and cervical cancer xenograft growth in vivo. Conversely, DANCR knockdown inhibits cervical cancer cell proliferation in vitro and cervical cancer xenograft growth in vivo. Therefore, our data demonstrated that DANCR also functions as an oncogene in cervical cancer, further supporting DANCR as a cancer-associated lncRNA. Our findings also implied that DANCR might be a promising prognostic biomarker and therapeutic target for cervical cancer.
In this study, we identified a novel mechanism mediating the oncogenic roles of DANCR in cervical cancer, which is the activation of the Wnt/β-catenin signalling pathway via upregulation of FRAT1 and FRAT2. Both public available TCGA data and cervical cancer tissues we collected display that the expression of FRAT1 and FRAT2 are positively associated with the expression of DANCR in cervical cancer tissues, supporting the positive regulation of FRAT1 and FRAT2 by DANCR. FRAT1 and FRAT2 belong to the GSK-3-binding protein family, inhibit GSK-3-mediated β-catenin phosphorylation and degradation, promote nuclear translocation of β-catenin, and activate Wnt/β-catenin signaling pathway [
44]. Indocyanine Green-001 (ICG-001) is an antagonist of β-catenin that specifically downregulates the expression of responsive genes of β-catenin [
48]. Thus, we used ICG-001 to inhibit Wnt/β-catenin signaling pathway in the functional assays, which led to the abolishment of the pro-proliferation of cervical cancer cells caused by DANCR overexpression and the anti-proliferatory roles of DNACR knockdown in cervical cancer cells. The results of functional experiments suggest that the effects of DANCR on cervical cancer cells are dependent on the activation of Wnt/β-catenin signaling pathway. DANCR has previously been reported to activate the Wnt/β-catenin signaling pathway in hepatocellular carcinoma, gastric cancer, and glioma [
32,
35,
48]. However, the detailed mechanisms underpinning the activation of the Wnt/β-catenin signaling pathway by DANCR in gastric cancer and glioma are unreported 9 [
35,
49]. In hepatocellular carcinoma, Yuan et al. reported that DANCR directly bound to β-catenin mRNA and inhibited β-catenin mRNA degradation [
32]. Several recent studies have reported the role of DANCR in cervical cancer associated with certain miRNAs [
50,
51], however whether DANCR also affects the Wnt/β-catenin signalling pathway in cervical cancer has not been revealed. In this present study, we provide a novel insight that the activation of the Wnt/β-catenin signalling pathway by DANCR is associated with cervical cancer progression. In addition, we also identify that DANCR regulates the expression levels of FRAT1 and FRAT2, which are regulators of Wnt/β-catenin signalling pathway.
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