Background
Methods
Ethic statement
Study population
Questionnaire
SNP selection and genotyping
Statistical analysis
Results
Variables | Total (N = 600) n* (%) | PTB patients (N = 200) n* (%) | LTBI controls (N = 200) n* (%) | Healthy controls without MTB infection (N = 200) n* (%) | p for difference |
---|---|---|---|---|---|
Age
| |||||
18–19 years | 48 (8.0) | 16 (8.0) | 15 (7.5) | 17 (8.5) | |
20–24 years | 183 (30.5) | 61 (30.5) | 62 (31.0) | 60 (30.0) | |
25–29 years | 68 (11.3) | 23 (11.5) | 23 (11.5) | 22 (11.0) | |
30–34 years | 50 (8.3) | 16 (8.0) | 16 (8.0) | 18 (9.0) | |
35–39 years | 60 (10.0) | 20 (10.0) | 20 (10.0) | 20 (10.0) | |
40–44 years | 48 (8.0) | 16 (8.0) | 16 (8.0) | 16 (8.0) | |
45–49 years | 65 (10.8) | 22 (11.0) | 22 (11.0) | 21 (10.5) | |
≥50 years | 78 (13.0) | 26 (13.0) | 26 (13.0) | 26 (13.0) | |
Gender
| |||||
Male | 333 (55.5) | 111 (55.5) | 111 (55.5) | 111 (55.5) | |
Female | 267 (44.5) | 89 (44.5) | 89 (44.5) | 89 (44.5) | |
Education
| |||||
≤9 years | 109 (18.2) | 71 (35.5) | 16 (8.0) | 22 (11.0) | <0.01§
|
10–12 years | 139 (23.2) | 55 (27.5) | 31 (15.5) | 53 (26.5) | <0.01&
|
>12 years | 352 (58.7) | 74 (37.0) | 153 (76.5) | 125 (62.5) | |
Per capita income in the family
| |||||
<500 Yuan/Month | 109 (18.2) | 59 (29.5) | 24 (12.0) | 26 (13.0) | <0.01§
|
500-2000 Yuan/Month | 254 (42.3) | 73 (36.5) | 96 (48.0) | 85 (42.5) | <0.01&
|
≥2000 Yuan/Month | 237 (39.5) | 68 (34.0) | 80 (40.0) | 89 (44.5) | |
Smoke status
| |||||
Never smoker | 429 (71.5) | 131 (65.5) | 146 (73.0) | 152 (76.0) | 0.10§
|
Former smoker | 72 (12.0) | 38 (19.0) | 23 (11.5) | 11 (5.5) | <0.01&
|
Current smoker | 99 (16.4) | 31 (15.5) | 31 (15.5) | 37 (18.5) | |
Current alcohol drinking
| |||||
No | 236 (39.4) | 79 (39.5) | 86 (43.2) | 71 (35.5) | 0.45§
|
Yes | 363 (60.0) | 121 (60.5) | 113 (56.8) | 129 (64.5) | 0.41&
|
BMI
| |||||
≤18.5 (underweight) | 78 (12.2) | 42 (21.0) | 17 (8.5) | 14 (7.0) | <0.01§
|
18.5-25 (normal) | 412 (68.7) | 146 (73.0) | 128 (64.0) | 138 (69.0) | <0.01&
|
>25 (overweight) | 115 (19.2) | 12 (6.0) | 55 (27.5) | 48 (24.0) | |
Is there TB patient in the family
| |||||
No | 550 (91.8) | 179 (89.9) | 185 (92.5) | 186 (93.0) | 0.37§
|
Yes | 49 (8.2) | 20 (10.1) | 15 (7.5) | 14 (7.0) | 0.28&
|
Number of scars by BCG vaccination
| |||||
0 | 242 (40.3) | 131 (65.5) | 66 (33.0) | 45 (22.5) | <0.01§
|
1 | 309 (51.5) | 60 (30.0) | 117 (58.5) | 132 (66.0) | <0.01&
|
≥2 | 49 (8.2) | 9 (4.5) | 17 (8.5) | 23 (11.5) |
Selected SNP | Genotype distribution* | p for difference | |||
---|---|---|---|---|---|
SNP (ID) | Genotype | PTB patients, n (%) | LTBI controls n (%) | Healthy controls without MTB infection n (%) | |
IL1RN
| TT | 170 (85.0) | 169 (85.8) | 173 (88.3) | 0.607§
|
9589 A/T | TA | 29 (14.5) | 28 (14.2) | 23 (11.7) | 0.432&
|
(rs454078) | AA | 1 (0.5) | 0 | 0 | |
IL1B
| CC | 54 (27.0) | 63 (32.0) | 59 (30.1) | 0.270§
|
-511 C/T | CT | 96 (48.0) | 97 (49.2) | 99 (50.5) | 0.393&
|
(rs16944) | TT | 50 (25.0) | 37 (18.8) | 38 (19.4) | |
IL2
| TT | 89 (44.5) | 90 (45.7) | 87 (44.4) | 0.972§
|
-330 T/G | TG | 88 (44.0) | 85 (43.2) | 88 (44.9) | 0.964&
|
(rs2069762) | GG | 23 (11.5) | 22 (11.2) | 21 (10.7) | |
IL4
| TT | 120 (60.0) | 106 (53.8) | 105 (53.6) | 0.456§
|
-589 C/T | TC | 70 (35.0) | 79 (40.1) | 77 (39.3) | 0.375&
|
(rs2243250) | CC | 10 (5.0) | 12 (6.1) | 14 (7.1) | |
IL8
| AA | 74 (37.0) | 75 (38.1) | 68 (34.7) | 0.967§
|
-251 T/A | AT | 97 (48.5) | 93 (47.2) | 99 (50.5) | 0.890&
|
(rs4073) | TT | 29 (14.5) | 29 (14.7) | 29 (14.8) | |
IL8RB
| TT | 83 (41.5) | 83 (42.1) | 78 (39.8) | 0.985§
|
1208 C/T | TC | 85 (42.5) | 82 (41.6) | 88 (44.9) | 0.891&
|
(rs1126579) | CC | 32 (16.0) | 32 (16.2) | 30 (15.3) | |
IL10
| TT | 99 (49.5) | 83 (42.1) | 81 (41.3) | 0.282§
|
-819 T/C | TC | 80 (40.0) | 94 (47.7) | 87 (44.4) | 0.217&
|
(rs1800871) | CC | 21 (10.5) | 20 (10.2) | 28 (14.3) | |
IL10
| TT | 176 (88.0) | 162 (82.2) | 160 (81.6) | 0.133§
|
-1082 A/G | TC | 23 (11.5) | 35 (17.8) | 35 (17.9) | 0.201&
|
(rs1800896) | CC | 1 (0.5) | 0 | 1 (0.5) | |
IL18
| CC | 157 (78.5) | 147 (74.6) | 152 (77.6) | 0.648§
|
-137 G/C | CG | 40 (20.0) | 46 (23.4) | 42 (21.4) | 0.865&
|
(rs187238) | GG | 3 (1.5) | 4 (2.0) | 2 (1.0) | |
IL18
| CC | 58 (29.0) | 50 (25.4) | 49 (25.0) | 0.708§
|
-607 A/C | CA | 99 (49.5) | 101 (51.3) | 101 (51.5) | 0.658&
|
(rs1946518) | AA | 43 (21.5) | 46 (23.4) | 46 (23.5) | |
TLR1
| GG | 90 (45.2) | 90 (35.4) | 74 (37.8) | 0.325§
|
-7202 A/G | GA | 84 (42.2) | 91 (32.7) | 103 (52.6) | 0.117&
|
(rs5743551) | AA | 25 (12.6) | 16 (8.1) | 19 (9.7) | |
TLR2
| TT | 97 (48.5) | 102 (51.8) | 97 (49.5) | 0.657§
|
597 C/T | TC | 83 (41.5) | 73 (37.1) | 81 (41.3) | 0.956&
|
(rs3804099) | CC | 20 (10.0) | 22 (11.2) | 18 (9.2) | |
TLR5
| CC | 189 (94.5) | 189 (95.9) | 188 (96.4) | 0.502§
|
1174 C/T | CT | 11 (5.5) | 8 (4.1) | 7 (3.6) | 0.377&
|
(rs5744168) | TT | 0 | 0 | 0 | |
TLR9
| AA | 70 (35.0) | 67 (34.0) | 68 (34.7) | 0.051§
|
1174 A/G | AG | 89 (44.5) | 106 (53.8) | 95 (48.5) | 0.592&
|
(rs352139) | GG | 41 (20.5) | 24 (12.2) | 33 (16.8) | |
TLR9
| GG | 72 (36.0) | 70 (35.5) | 71 (36.2) | 0.052§
|
1635 A/G | GA | 88 (44.0) | 104 (52.8) | 93 (47.5) | 0.608&
|
(rs352140) | AA | 40 (20.0) | 23 (11.7) | 32 (16.3) |
Selected SNP | Genotype distribution* | p for difference | |||
---|---|---|---|---|---|
Gene | SNP (ID) | PTB patients, n (%) | LTBI controls n (%) | Healthy controls without MTB infection n (%) | |
CCR2
| GG | 125 (62.5) | 125 (63.5) | 120 (61.2) | 0.397§
|
190 A/G | GA | 62 (31.0) | 65 (33.0) | 67 (34.2) | 0.612&
|
(rs1799864) | AA | 13 (6.5) | 7 (3.6) | 9 (4.6) | |
CCR5
| GG | 71 (35.5) | 62 (31.5) | 59 (30.1) | 0.511§
|
59029 A/G | GA | 97 (48.5) | 107 (54.3) | 102 (52.0) | 0.515&
|
(rs1799987) | AA | 32 (16.0) | 28 (14.2) | 35 (17.9) | |
RANTES
| GG | 77 (38.5) | 63 (32.0) | 74 (37.8) | 0.349§
|
-403 G/A | GA | 96 (48.0) | 101 (51.3) | 95 (48.5) | 0.988&
|
(rs2107538) | AA | 27 (13.5) | 33 (16.8) | 27 (13.8) | |
MCP1
| GG | 71 (35.5) | 64 (32.5) | 75 (38.3) | 0.632§
|
2518 A/G | GA | 96 (48.0) | 104 (52.8) | 88 (44.9) | 0.812&
|
(rs1024611) | AA | 33 (16.5) | 29 (14.7) | 33 (16.8) | |
NRAMP1
| GG | 151 (75.5) | 151 (76.7) | 139 (70.9) | 0.837§
|
D543N | GA | 47 (23.5) | 43 (21.8) | 55 (28.1) | 0.582&
|
(rs17235409) | AA | 2 (1.0) | 3 (1.5) | 2 (1.0) | |
P2X7
| AA | 116 (58.0) | 113 (57.4) | 109 (55.6) | 0.502§
|
1513 A/C | AC | 76 (38.0) | 71 (36.0) | 76 (38.8) | 0.722&
|
(rs3751143) | CC | 8 (4.0) | 13 (6.6) | 11 (5.6) | |
P2X7
| CC | 92 (46.2) | 86 (43.7) | 86 (43.9) | 0.835§
|
-762 T/C | CT | 81 (40.7) | 86 (43.7) | 88 (44.9) | 0.669&
|
(rs2393799) | TT | 26 (13.1) | 25 (12.7) | 22 (11.2) | |
TNF-α
| GG | 169 (84.5) | 178 (90.4) | 176 (89.8) | 0.210§
|
-308 G/A | GA | 29 (14.5) | 18 (9.1) | 19 (9.7) | 0.284&
|
(rs1800629) | AA | =2 (1.0) | 1 (0.5) | 1 (0.5) | |
IFNG
| AA | 74 (37.0) | 57 (28.9) | 73 (37.2) | 0.119§
|
2109 G/A | AG | 97 (48.5) | 99 (50.3) | 85 (43.4) | 0.374&
|
(rs1861494) | GG | 29 (14.5) | 41 (20.8) | 38 (19.4) | |
CD4
| CC | 188 (94.0) | 185 (93.9) | 184 (93.9) | 0.593§
|
868 C/T | CT | 11 (5.5) | 12 (6.1) | 12 (6.1) | 0.593&
|
(rs28919570) | TT | 1 (0.5) | 0 | 0 | |
CD14
| TT | 54 (27.1) | 66 (33.5) | 60 (30.6) | 0.331§
|
159 C/T | TC | 103 (51.7) | 97 (49.2) | 104 (53.1) | 0.438&
|
(rs2569190) | CC | 42 (21.1) | 34 (17.3) | 32 (16.3) | |
MBL
| CC | 150 (75.0) | 150 (76.1) | 141 (71.9) | 0.964§
|
-221 G/C | CG | 48 (24.0) | 45 (22.8) | 52 (26.5) | 0.742&
|
(rs7096206) | GG | 2 (1.0) | 2 (1.0) | 3 (1.5) | |
TIRAP
| CC | 196 (98.0) | 190 (96.5) | 188 (96.4) | 0.346§
|
539 C/T | CT | 4 (2.0) | 7 (3.6) | 7 (3.6) | 0.359&
|
(rs8177374) | TT | 0 | 0 | 0 | |
NFKBIA
| AA | 150 (75.0) | 148 (75.1) | 153 (78.1) | 0.999§
|
-881 A/G | AC | 48 (24.0) | 47 (23.8) | 43 (21.9) | 0.322&
|
(rs3138053) | CC | 2 (1.0) | 2 (1.0) | 0 | |
NFKBIA
| CC | 149 (74.9) | 148 (75.1) | 153 (78.1) | 0.998§
|
-826 C/T | CT | 48 (24.1) | 47 (23.8) | 43 (21.9) | 0.316&
|
(rs2233406) | TT | 2 (1.0) | 2 (1.0) | 0 |
Genotype | PTB patients, n (%) | LTBI controls n (%) | Adjusted OR* (95% CI) p value | Adjusted OR&(95% CI) p value | PTB patients n (%) | Healthy controls without MTB infection n (%) | Adjusted OR* (95% CI) p value | Adjusted OR&(95% CI) p value | |
---|---|---|---|---|---|---|---|---|---|
TLR9 1174 A/G (rs352139) | AA | 70 (35.0) | 67 (34.0) | Ref. | Ref. | 70 (35.0) | 68 (34.7) | Ref. | Ref. |
AG | 89 (44.5) | 106 (53.8) | 0.80 (0.52-1.25)p = 0.325 | 0.74 (0.44-1.25) p = 0.264 | 89 (44.5) | 95 (48.5) | 0.91 (0.58-1.41) p = 0.668 | 0.67 (0.39-1.15) p = 0.149 | |
GG | 41 (20.5) | 24 (12.2) | 1.64 (0.19-3.01) p = 0.110 | 1.72 (0.81-3.64) p = 0.155 | 41 (20.5) | 33 (16.8) | 1.21 (0.69-2.13) p = 0.511 | 0.98 (0.49-1.96) p = 0.953 | |
Dominant | GG + AG vs AA | 0.96 (0.63-1.45) p = 0.833 | 0.90 (0.55-1.48) p = 0.687 | 0.99 (0.65-1.49) p = 0.949 | 0.75 (0.45-1.24) p = 0.263 | ||||
Recessive | GG vs AG + AA |
1.87 (1.08-3.23) p = 0.027
|
2.04 (1.02-4.06) p = 0.043
| 1.28 (0.77-2.13) p = 0.346 | 1.22 (0.65-2.27) p = 0.536 | ||||
TLR9 1635 A/G (rs352140) | GG | 72 (36.0) | 70 (35.5) | Ref. | Ref. | 72 (36.0) | 71 (36.2) | Ref. | Ref. |
GA | 88 (44.0) | 104 (52.8) | 0.82 (0.53-1.27) p = 0.377 | 0.80 (0.47-1.34) p = 0.391 | 88 (44.0) | 93 (37.5) | 0.93 (0.60-1.45) p = 0.749 | 0.72 (0.42-1.23) p = 0.223 | |
AA | 40 (20.0) | 23 (11.7) | 1.70 (0.92-3.12) p = 0.091 | 1.74 (0.82-3.69) p = 0.152 | 40 (20.0) | 32 (16.3) | 1.24 (0.70-2.18) p = 0.468 | 1.00 (0.50-2.00) p = 1.00 | |
Dominant | AA + GA vs GG | 0.98 (0.65-1.48) p = 0.920 | 0.95 (0.58-1.56) p = 0.839 | 1.01 (0.67-1.52) p = 0.964 | 0.79 (0.48-1.30) p = 0.353 | ||||
Recessive | AA vs GA + GG |
1.90 (1.09-3.31) p = 0.025
| 1.97 (0.98-3.96) p = 0.057 | 1.29 (0.77-2.15) p = 0.341 | 1.20 (0.64-2.45) p = 0.579 | ||||
IFNG 2109 G/A (rs1861494) | AA | 74 (37.0) | 57 (28.9) | Ref. | Ref. | 74 (37.0) | 73 (37.2) | Ref. | Ref. |
AG | 97 (48.5) | 99 (50.3) | 0.75 (0.48-1.18) p = 0.212 | 0.57 (0.33-0.99) p = 0.045 | 97 (48.5) | 85 (43.4) | 1.13 (0.73-1.74) p = 0.591 | 1.22 (0.71-2.07) p = 0.470 | |
GG | 29 (14.5) | 41 (20.8) |
0.54 (0.30-0.98) p = 0.042
|
0.44 (0.21-0.91) p = 0.027
| 29 (14.5) | 38 (19.4) | 0.75 (0.42-1.34) p = 0.334 | 0.74 (0.37-1.51) p = 0.413 | |
Dominant | GG + AG vs AA | 0.69 (0.45-1.06) p = 0.087 |
0.53 (0.32-0.90) p = 0.018
| 1.01 (0.67-1.52) p = 0.960 | 1.06 (0.65-1.74) p = 0.814 | ||||
Recessive | GG vs AG + AA | 0.65 (0.38-1.09) p = 0.100 | 0.62 (0.33-1.18) p = 0.145 | 0.70 (0.41-1.20) p = 0.194 | 0.67 (0.35-1.28) p = 0.228 |
TLR91174 A/G |
IFNG2109G/A | PTB patients, n (%) | LTBI controls n (%) | Adjusted OR* (95% CI) p value | Adjusted OR&(95% CI) p value | PTB patients, n (%) | Healthy controls without MTB infection n (%) | Adjusted OR* (95% CI) p value | Adjusted OR&(95% CI)p value |
---|---|---|---|---|---|---|---|---|---|
AA/AG | GG/GA | 97 (48.5) | 122 (61.9) | Ref. | Ref. | 97 (48.5) | 103 (52.6) | Ref. | Ref. |
AA/AG | AA | 62 (31.0) | 51 (25.9) | 1.54 (0.97-2.43) p = 0.065 |
1.97 (1.12-3.47) p = 0.019
| 62 (31.0) | 60 (30.6) | 1.10 (0.70-1.73) p = 0.683 | 1.02 (0.59-1.77) p = 0.941 |
G/G | GG/GA | 29 (14.5) | 18 (9.1) |
2.04 (1.07-3.91) p = 0.031
| 2.17 (0.96-4.91) p = 0.062 | 29 (14.5) | 20 (10.2) | 1.55 (0.82-2.94) p = 0.178 | 1.41 (0.65-3.08) p = 0.399 |
G/G | A/A | 12 (6.0) | 6 (3.1) | 2.53 (0.92-7.00) p = 0.073 |
4.31 (0.21-15.31) p = 0.024
| 12 (6.0) | 13 (6.6) | 0.98 (0.43-2.25) p = 0.965 | 0.95 (0.34-2.62) p = 0.918 |
p for trend |
0.004
|
0.002
| 0.433 | 0.697 |