Background
Methods
Strains in this study
Susceptibility testing
Pulsed-field gel electrophoresis
Confirmation of KPC production and KPC types by sequencing
Conjugation experiments
PCR detection of virulence-associated genes rmpA and aerobactin, and serotypes K1, K2, and K5
Multilocus sequence typing (MLST)
Virulence studies
Results
Conjugation of plasmids of KPC-2 and -3 into serotype recipient K2 and antimicrobial susceptibility test of their transconjugants
Antibiotics | Recipient K2 | Donor | Transconjugant | ||
---|---|---|---|---|---|
KPC-2 | KPC-3 | KPC-2 | KPC-3 | ||
Ampicillin | ≥32 | ≥32 | ≥32 | ≥32 | ≥32 |
Cefazolin | ≤2 | ≥32 | ≥32 | ≥32 | ≥32 |
Cefoxitin | ≤4 | ≥128 | ≥128 | ≥128 | ≥128 |
Aztreonam | ≤1 | ≥32 | ≥32 | ≥32 | ≥32 |
Cefotaxime | ≤1 | 32 | 32 | 8 | 8 |
Ceftazidime | ≤1 | ≥32 | ≥32 | ≥32 | ≥32 |
Cefepime | ≤1 | ≥32 | 16 | 16 | 8 |
Ertapenem | ≤0.25 | ≥8 | ≥8 | ≥8 | ≥8 |
Imipenem | 0.5 | ≥8 | ≥8 | ≥8 | ≥8 |
Meropenem | ≤0.25 | ≥8 | ≥8 | ≥8 | ≥8 |
Doripenem | ≤0.12 | ≥4 | ≥4 | ≥4 | ≥4 |
Colistin | 1 | ≤0.5 | ≥4 | ≤1 | 1 |
Tigecycline | ≤0.25 | 1 | ≤0.25 | ≤0.25 | ≤0.25 |
Gentamicin | ≤1 | ≤1 | ≥16 | ≤1 | ≤1 |
Amikacin | ≤4 | 16 | 32 | ≤4 | ≤4 |
Ciprofloxacin | 0.12 | ≥4 | ≥4 | ≤0.06 | 0.12 |
Levofloxacin | ≤0.5 | ≥8 | ≥8 | ≤0.5 | ≤0.5 |
SXT* | ≤0.5 | ≥4 | ≥4 | ≥4 | ≤0.5 |