Background
Methods
Patients and Samples
Cell culture
DNA extraction
PCR-restriction fragment length polymorphism analysis of BCL2 -938 polymorphism
Immunohistochemistry (IHC) and evaluation of IHC staining
Western-blot analysis of Bcl-2 expression in breast cancer cell lines
Statistical Analysis
Results
The BCL-2(-938C > A) genotype distribution
Genotype | No. of subjects (%) | Non-adjusted b
| Adjusted c
| |||
---|---|---|---|---|---|---|
Case a
| Control a
|
pvalue | OR |
pvalue | OR | |
AA | 19(16.7) | 8(7.5) | 1.00 | 1.00 | ||
AC | 53(46.5) | 53(49.5) | 0.062 | 0.42 | 0.081 | 0.44 |
CC | 42(36.8) | 46(43.0) | 0.043 | 0.38 | 0.054 | 0.40 |
AC+CC | 95(83.3) | 99(92.5) | 1.00 | 1.00 | ||
AA | 19(16.7) | 8(7.5) | 0.042 | 2.47 | 0.055 | 2.37 |
AC+AA | 72(63.2) | 61(57.0) | 1.00 | 1.00 | ||
CC | 42(36.8) | 46(43.0) | 0.351 | 0.77 | 0.358 | 0.77 |
AA+CC | 61(53.5) | 54(50.5) | 1.00 | 1.00 | ||
AC | 53(46.5) | 53(49.5) | 0.651 | 0.89 | 0.711 | 0.90 |
All | 114 | 107 |
Association between BCL-2(-938C > A) polymorphism and known clinicopathological variables
Clinicopathological Variables | All | Genotype (%) | Adjusted a
| ||
---|---|---|---|---|---|
AA | AC+CC |
pvalue | OR | ||
Age | |||||
≤ 40 | 21(18.9) | 3(14.3) | 18(85.7) | 1.00 | |
> 40 | 90(81.1) | 15(16.7) | 75(83.3) | 0.790 | 1.20 |
Pathological diagnosis | |||||
Ductal | 87(76.3) | 14(16.1) | 73(83.9) | 1.00 | |
Lobular | 7(6.1) | 3(42.9) | 4(57.1) | 0.036 | 6.42 |
Others | 20(17.6) | 2(10.0) | 18(90.0) | 0.605 | 0.66 |
Tumor size(cm) | |||||
≤ 2 | 50(53.2) | 9(18.0) | 41(82.0) | 1.00 | |
> 2 | 44(46.8) | 9(20.4) | 35(79.6) | 0.704 | 1.23 |
Grade | |||||
I, II | 59(79.7) | 10(16.9) | 49(83.1) | 1.00 | |
III | 15(20.3) | 2(13.3) | 13(86.7) | 0.725 | 0.74 |
Positive lymph node | |||||
0 | 34(44.7) | 3(8.8) | 31(91.2) | 1.00 | |
1-3 | 25(32.9) | 3(12.0) | 22(88.0) | 0.672 | 1.44 |
≥ 4 | 17(22.4) | 7(41.2) | 10(58.8) | 0.014 | 6.95 |
ER | |||||
Negative | 28(26.9) | 6(21.4) | 22(78.6) | 1.00 | |
Positive | 76(73.1) | 12(15.8) | 64(84.2) | 0.493 | 0.68 |
PR | |||||
Negative | 37(35.9) | 8(21.6) | 29(78.4) | 1.00 | |
Positive | 66(64.1) | 10(15.2) | 56(84.8) | 0.688 | 0.80 |
Receptor status | |||||
Negative | 26(25.2) | 6(23.1) | 20(76.9) | 1.00 | |
Positive | 77(74.8) | 12(15.6) | 65(84.4) | 0.115 | 3.06 |
c-erbB2
| |||||
Negative | 73(70.2) | 13(17.8) | 60(82.2) | 1.00 | |
Positive | 31(29.8) | 5(16.1) | 26(83.9) | 0.936 | 1.05 |
Triple-negative | |||||
No | 87(84.5) | 14(16.1) | 73(83.9) | 1.00 | |
Yes | 16(15.5) | 4(25.0) | 12(75.0) | 0.444 | 1.67 |
Ki67
| |||||
Negative | 59(58.4) | 10(16.9) | 49(83.1) | 0.875 | 1.00 |
Positive | 42(41.6) | 7(16.7) | 35(83.3) | 1.09 |