Background
Methods
Blood samples
RNA purification
miRNA qRT-PCR
Statistical analysis
Results
Statistical analysis of patients’ characteristics
CHS
|
Age (YEARS)
|
M/F
|
CS (%)
|
TC
|
TG
|
HDL
|
LDL
|
SBP
|
DBP
|
DM
|
HT (%)
|
HL (%)
|
P
|
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Stroke (24 h)
| |||||||||||||
LA (n = 10) | 62 ± 7 | 5/5 | 2 (20%) | 4.48 ± 0.71 | 1.53 ± 0.43 | 1.13 ± 0.18 | 2.53 ± 0.58 | 132 ± 12 | 84 ± 13 | 1 (10%) | 2 (20%) | 2 (20%) | 0.12 |
SA (n = 9) | 63 ± 6 | 4/5 | 2 (22%) | 4.32 ± 0.63 | 1.43 ± 0.23 | 1.24 ± 0.16 | 2.42 ± 0.61 | 125 ± 10 | 82 ± 14 | 2 (22%) | 2 (22%) | 1 (11%) | 0.32 |
CEmb (n = 9) | 64 ± 5 | 5/4 | 1 (11%) | 4.18 ± 0.52 | 1.23 ± 0.18 | 1.02 ± 0.12 | 2.43 ± 0.47 | 130 ± 12 | 85 ± 11 | 1 (11%) | 1 (11%) | 1 (11%) | 0.46 |
UDN (n = 10) | 61 ± 6 | 5/5 | 2 (20%) | 4.23 ± 0.34 | 1.36 ± 0.33 | 1.25 ± 0.27 | 2.45 ± 0.45 | 135 ± 13 | 80 ± 13 | 1 (10%) | 2 (20%) | 2 (20%) | 0.78 |
Stroke (1 W)
| |||||||||||||
LA (n = 11) | 65 ± 6 | 5/6 | 2 (18%) | 4.12 ± 0.25 | 1.54 ± 0.46 | 1.15 ± 0.12 | 2.50 ± 0.35 | 128 ± 14 | 82 ± 10 | 1 (9%) | 2 (18%) | 2 (20%) | 0.98 |
SA (n = 10) | 66 ± 4 | 5/5 | 2 (20%) | 4.16 ± 0.32 | 1.44 ± 0.35 | 1.10 ± 0.17 | 2.43 ± 0.42 | 138 ± 27 | 86 ± 14 | 1 (10%) | 2 (20%) | 1 (10%) | 0.54 |
CEmb (n = 11) | 62 ± 7 | 6/5 | 2 (18%) | 4.31 ± 0.17 | 1.32 ± 0.38 | 1.15 ± 0.14 | 2.46 ± 0.38 | 137 ± 10 | 85 ± 13 | 1 (9%) | 1 (9%) | 1 (9%) | 0.65 |
UDN (n = 10) | 63 ± 7 | 4/6 | 2 (20%) | 4.42 ± 0.54 | 1.42 ± 0.43 | 1.17 ± 0.12 | 2.35 ± 0.36 | 132 ± 15 | 87 ± 11 | 1 (10%) | 1 (10%) | 1 (10%) | 0.98 |
Stroke (4 W)
| |||||||||||||
LA (n = 10) | 65 ± 5 | 6/4 | 2 (20%) | 4.39 ± 0.47 | 1.55 ± 0.39 | 1.18 ± 0.13 | 2.62 ± 0.56 | 130 ± 12 | 88 ± 10 | 1 (10%) | 2 (20%) | 2 (20%) | 0.82 |
SA (n = 10) | 64 ± 7 | 4/6 | 1 (10%) | 4.36 ± 0.52 | 1.36 ± 0.36 | 1.09 ± 0.13 | 2.43 ± 0.34 | 129 ± 16 | 85 ± 11 | 2 (20%) | 2 (20%) | 1 (10%) | 0.12 |
CEmb (n = 10) | 63 ± 7 | 5/5 | 2 (20%) | 4.26 ± 0.17 | 1.38 ± 0.29 | 1.12 ± 0.17 | 2.43 ± 0.33 | 130 ± 12 | 86 ± 10 | 1 (10%) | 1 (10%) | 1 (10%) | 0.12 |
UDN (n = 10) | 64 ± 6 | 6/4 | 2 (20%) | 4.24 ± 0.43 | 1.42 ± 0.35 | 1.19 ± 0.16 | 2.44 ± 0.44 | 127 ± 13 | 82 ± 12 | 1 (10%) | 2 (20%) | 1 (10%) | 0.34 |
Stroke (24 W)
| |||||||||||||
LA (n = 10) | 65 ± 7 | 6/4 | 1 (10%) | 4.36 ± 0.27 | 1.56 ± 0.42 | 1.14 ± 0.15 | 2.43 ± 0.56 | 126 ± 15 | 88 ± 12 | 1 (10%) | 2 (20%) | 2 (20%) | 0.87 |
SA (n = 9) | 66 ± 6 | 4/5 | 1 (11%) | 4.39 ± 0.59 | 1.44 ± 0.32 | 1.17 ± 0.12 | 2.39 ± 0.45 | 124 ± 13 | 83 ± 11 | 2 (22%) | 2 (22%) | 1 (11%) | 0.23 |
CEmb (n = 10) | 64 ± 5 | 5/5 | 1 (10%) | 4.35 ± 0.49 | 1.37 ± 0.41 | 1.15 ± 0.16 | 2.50 ± 0.43 | 126 ± 15 | 85 ± 12 | 1 (10%) | 1 (10%) | 1 (10%) | 0.13 |
UDN (n = 9) | 65 ± 6 | 5/4 | 1 (11%) | 429 ± 0.39 | 1.49 ± 0.29 | 1.19 ± 0.18 | 2.45 ± 0.45 | 125 ± 13 | 86 ± 11 | 1 (11%) | 1 (11%) | 1 (11%) | 0.56 |
Stroke (48 W)
| |||||||||||||
LA (n = 10) | 64 ± 7 | 6/4 | 2 (20%) | 4.46 ± 0.28 | 1.57 ± 0.42 | 1.15 ± 0.13 | 2.55 ± 0.43 | 127 ± 12 | 84 ± 12 | 1 (10%) | 2 (20%) | 2 (20%) | 0.11 |
SA (n = 10) | 66 ± 5 | 5/5 | 2 (20%) | 4.35 ± 0.42 | 1.43 ± 0.32 | 1.17 ± 0.20 | 2.46 ± 0.53 | 123 ± 11 | 83 ± 10 | 2 (20%) | 1 (10%) | 1 (10%) | 0.45 |
CEmb (n = 10) | 65 ± 7 | 4/6 | 1 (10%) | 4.37 ± 0.42 | 1.38 ± 0.41 | 1.23 ± 0.15 | 2.61 ± 0.53 | 125 ± 10 | 87 ± 14 | 1 (10%) | 1 (10%) | 1 (10%) | 0.55 |
UDN (n = 9) | 64 ± 8 | 5/4 | 1 (11%) | 4.44 ± 0.32 | 1.42 ± 0.33 | 1.17 ± 0.18 | 2.65 ± 0.62 | 129 ± 13 | 87 ± 13 | 1 (11%) | 1 (10%) | 1 (10%) | 0.87 |
Healthy outpatient (n = 50)
| 64 ± 6 | 24/26 | 10 (20%) | 4.52 ± 0.48 | 1.39 ± 0.40 | 1.15 ± 0.14 | 2.50 ± 0.62 | 125 ± 11 | 82 ± 11 | 5 (10%) | 5 (10%) | 6 (12%) |
MiRNAs plasma levels in ischemic stroke patients and healthy volunteers
STROKE | HA ΔCT ± SD | |||||
---|---|---|---|---|---|---|
24 h | 1 w | 4 w | 24 w | 48 w | ||
miR-30a
| −6.18 ± 1.27 | |||||
LA Δct ± SD
| −4.42 ± 0.57 | −4.12 ± 0.43 | −4.53 ± 0.46 | −4.65 ± 0.47 | −6.02 ± 0.45 | |
SA Δct ± SD
| −4.15 ± 0.43 | −4.36 ± 0.18 | −4.63 ± 0.35 | −4.38 ± 0.35 | −5.97 ± 0.34 | |
CEmb Δct ± SD
| −4.79 ± 0.40 | −4.71 ± 0.15 | −5.26 ± 0.26 | −5.7 ± 0.42 | −6.12 ± 0.32 | |
UND Δct ± SD
| −3.94 ± 0.32 | −4.91 ± 0.28 | −5.33 ± 0.29 | −4.29 ± 0.49 | −6.05 ± 0.19 | |
p value
| 0.02 | 0.01 | 0.03 | 0.01 | 0.01 | |
miR-126
| 0.39 ± 0.012 | |||||
LA Δct ± SD
| 4.51 ± 1.25 | 4.28 ± 1.13 | 3.95 ± 1.05 | 3.45 ± 1.03 | 0.41 ± 0.023 | |
SA Δct ± SD
| 4.38 ± 1.12 | 4.16 ± 1.15 | 3.75 ± 1.12 | 2.95 ± 0.23 | 0.36 ± 0.013 | |
CEmb Δct ± SD
| 4.57 ± 1.32 | 4.08 ± 0.98 | 3.86 ± 0.89 | 3.15 ± 0.43 | 0.37 ± 0.021 | |
UND Δct ± SD
| 4.49 ± 1.08 | 4.12 ± 1.02 | 3.69 ± 0.97 | 1.98 ± 0.38 | 0.42 ± 0.032 | |
p value
| 0.01 | 0.01 | 0.02 | 0.03 | 0.01 | |
Let-7b
| −11.12 ± 2.16 | |||||
LA Δct ± SD
| −9.12 ± 1.25 | −9.36 ± 1.06 | −9.28 ± 1.34 | −9.31 ± 1.13 | −11.15 ± 1.22 | |
SA Δct ± SD
| −13.98 ± 2.15 | −14.18 ± 1.32 | −13.46 ± 1.45 | −13.12 ± 1.42 | −11.01 ± 1.08 | |
CEmb Δct ± SD
| −14.36 ± 2.18 | −14.78 ± 1.95 | −14.12 ± 1.53 | −13.92 ± 1.67 | −10.93 ± 1.52 | |
UND Δct ± SD
| −14.23 ± 1.58 | −14.97 ± 1.78 | −14.67 ± 1.36 | −12.93 ± 1.76 | −10.96 ± 1.63 | |
p value
| 0.01 | 0.02 | 0.01 | 0.01 | 0.01 |
Specifity and sensitivity of miRNAs
95% CI | AUC | Cut-off point | Specificity (%) | Sensitivity (%) | |
---|---|---|---|---|---|
miR-30a
| |||||
24 h
| 0.869–0.979 | 0.91 | 1.675 | 94 | 80 |
1 W
| 0.848–0.971 | 0.91 | 1.75 | 93 | 84 |
4 W
| 0.856–0.976 | 0.92 | 1.67 | 90 | 84 |
24 W
| 0.875–0.984 | 0.93 | 1.665 | 92 | 84 |
miR-126
| |||||
24 h
| 0.871–0.978 | 0.92 | 1.75 | 92 | 84 |
1 W
| 0.895–0.985 | 0.94 | 1.875 | 90 | 86 |
4 W
| 0.878–0.982 | 0.93 | 1.845 | 92 | 84 |
24 W
| 0.864–0.977 | 0.92 | 1.77 | 92 | 82 |
Let-7b
| |||||
24 h
| 0.879–0.980 | 0.93 | 1.675 | 92 | 84 |
1 W
| 0.866–0.98 | 0.92 | 1.665 | 90 | 84 |
4 W
| 0.858–0.98 | 0.92 | 1.66 | 92 | 86 |
24 W
| 0.849–0.97 | 0.91 | 1.605 | 89 | 80 |