Background
Methods
Patients and specimens
Characteristics | No. of patients | ALDH1A1 expression (%) |
P-value | |
---|---|---|---|---|
Negative | Positive | |||
Gender | ||||
Male | 140 | 64 (45.7%) | 76 (54.3%) | |
Female | 76 | 44 (57.9%) | 32 (42.1%) | 0.087 |
Age (years) | ||||
≤ 60 | 136 | 74 (54.4%) | 62 (45.6%) | |
> 60 | 80 | 34 (42.5%) | 46 (57.5%) | 0.091 |
Size (cm) | ||||
≤ 5.0 | 139 | 76 (54.7%) | 63 (45.3%) | |
> 5.0 | 77 | 32 (41.6%) | 45 (58.4%) | 0.065 |
Tumor site | ||||
Upper | 86 | 36 (41.9%) | 50 (58.1%) | |
Middle/Lower | 130 | 72 (55.4%) | 58 (44.6%) | 0.052 |
Differentiation | ||||
Well/Moderate | 96 | 41 (42.7%) | 55 (57.3%) | |
Poor | 120 | 67 (55.8%) | 53 (44.2%) | 0.055 |
Depth of invasion | ||||
T1/T2 | 81 | 58 (71.6%) | 23 (28.4%) | |
T3/T4 | 135 | 50 (37.0%) | 85 (63.0%) | < 0.001 |
Lymph node metastasis | ||||
Negative | 59 | 46 (78.0%) | 13 (22.0%) | |
Positive | 157 | 62 (39.5%) | 95 (60.5%) | < 0.001 |
Stages | ||||
I/II | 71 | 52 (73.2%) | 19 (26.8%) | |
III | 145 | 56 (38.6%) | 89 (61.4%) | < 0.001 |
Immunohistochemistry staining
Follow-up
Statistical analysis
Results
The association of ALDH1A1 with clinicopathological variables
Effect of tumor ALDH1A1 protein level on prognosis
Variables | No. of patients | OS rate (%) |
P-value | RFS rate (%) |
P-value | ||
---|---|---|---|---|---|---|---|
3 y | 5 y | 3 y | 5 y | ||||
Gender | |||||||
Male | 140 | 46.9 | 39.6 | 41.5 | 35.4 | ||
Female | 76 | 54.0 | 43.1 | 0.308 | 44.4 | 41.2 | 0.381 |
Age (years) | |||||||
≤ 60 | 136 | 49.8 | 43.9 | 45.3 | 42.2 | ||
> 60 | 80 | 48.9 | 36.1 | 0.338 | 38.1 | 30.6 | 0.391 |
Size (cm) | |||||||
≤ 5.0 | 139 | 55.6 | 47.4 | 50.4 | 45.5 | ||
> 5.0 | 77 | 38.1 | 28.8 | 0.001 | 28.3 | 23.3 | < 0.001 |
Tumor site | |||||||
Upper | 86 | 45.9 | 33.8 | 34.6 | 28.6 | ||
Middle/Lower | 130 | 51.8 | 45.7 | 0.074 | 47.8 | 43.7 | 0.047 |
Differentiation | |||||||
Well/Moderate | 96 | 47.8 | 39.7 | 41.2 | 34.6 | ||
Poor | 120 | 50.7 | 41.6 | 0.673 | 43.6 | 40.1 | 0.822 |
Depth of invasion | |||||||
T1/T2 | 81 | 71.4 | 66.5 | 70.2 | 65.3 | ||
T3/T4 | 135 | 36.2 | 25.5 | < 0.001 | 25.6 | 20.8 | < 0.001 |
Lymph node metastasis | |||||||
Negative | 59 | 83.5 | 79.2 | 82.0 | 77.5 | ||
Positive | 157 | 36.5 | 26.6 | < 0.001 | 27.5 | 22.6 | < 0.001 |
ALDH1A1 protein expression | |||||||
Negative | 108 | 69.3 | 57.1 | 60.5 | 54.8 | ||
Positive | 108 | 28.7 | 23.9 | < 0.001 | 23.7 | 19.2 | < 0.001 |
Variables | β | SE | Hazard ratio (95% CI) |
P-valuea
|
---|---|---|---|---|
OS | ||||
Tumor size | 0.410 | 0.177 | 1.507 (1.065 ~ 2.133) | 0.021 |
Depth of invasion | 0.576 | 0.255 | 1.779 (1.079 ~ 2.931) | 0.024 |
Lymph node metastasis | 1.018 | 0.341 | 2.767 (1.417 ~ 5.403) | 0.003 |
ALDH1A1 protein expression | 0.712 | 0.189 | 2.037 (1.407 ~ 2.950) | < 0.001 |
RFS | ||||
Tumor size | 0.411 | 0.178 | 1.508 (1.064 ~ 2.138) | 0.021 |
Tumor site | 0.043 | 0.180 | 1.044 (0.733 ~ 1.486) | 0.813 |
Depth of invasion | 0.649 | 0.256 | 1.913 (1.158 ~ 3.162) | 0.011 |
Lymph node metastasis | 1.063 | 0.338 | 2.894 (1.491 ~ 5.619) | 0.002 |
ALDH1A1 protein expression | 0.665 | 0.188 | 1.945 (1.346 ~ 2.812) | < 0.001 |