Background
Why is there still relevance for a new population-based cohort study?
Scope of research
Methods
Study area, collaboration and overview
LIFE Child HEALTH cohort | LIFE Child OBESITY cohort | |||
---|---|---|---|---|
Participant age
|
0-2 years
|
2-6 years
|
≥ 6 years
|
≥ 6 years
|
Anthropometry
| · Weight | · Weight | · Weight | · Weight |
· Height | · Height and sitting height | · Height and sitting height | · Height and sitting height | |
· Head, arm, leg, waist, neck, hip, thoracal and abdominal circumference | · Head, arm, leg, waist, neck and hip circumference | · Head, arm, leg, waist, neck and hip circumference | · Head, arm, leg, waist, neck and hip circumference | |
· Biparietal diameter | · Skinfolds | · Skinfolds | · Skinfolds | |
· Blood pressure | · Blood pressure | · Blood pressure | · Blood pressure | |
Clinical exam
| · Clinical history (perinatal and past medical history, medications, immunisations, developmental history) | · Clinical history (perinatal and past medical history, medications, immunisations, developmental history) | · Clinical history (perinatal and past medical history, medications, immunisations, developmental history) | · Clinical history (perinatal and past medical history, medications, immunisations, developmental history, specific details relating to weight) |
· Clinical examination including neurological examination and pubertal assessment | · Clinical examination including neurological examination and pubertal assessment | · Clinical examination including neurological examination and pubertal assessment | · Clinical examination including neurological examination and pubertal assessment | |
· Dental examination including dentition and early childhood caries | · Dental examination including dentition and early childhood caries | · Dental examination (jaw models, dental caries, periodontal disease, orofacial pain, bruxism and tongue function) | · Dental examination (jaw models, dental caries, periodontal disease, orofacial pain, bruxism and tongue function) | |
Biological samples
| · Blood, urine and hair | · Blood, urine and hair | · Blood, urine and hair | · Oral glucose tolerance test |
· Blood, urine and hair | ||||
Questionnaires
| · See Table 4
| · See Table 4
| · See Table 4
| · See Table 4
|
Motor and cognitive development
| · Bayley scales | · Bayley scales (< 3.6 years) | · Motor skills test | · Motor skills test |
· Motor skills test (> 3.6 years) | ||||
Imaging
| · Cranial ultrasound (3rd month of life) | · 3D-Bodyscan1
| · 3D-Bodyscan1
| |
· Sonography (heart, intima media thickness, thyroid and kidney2, for a sub-sample) | · Sonography (heart, intima media thickness, thyroid and kidney2) | |||
· Magnetic resonance imaging (for a sub-sample2) | ||||
Cardiovascular and pulmonary function tests
| Spirometry | · Spiroergometry | ||
· Spirometry | ||||
· Endopat (for a sub-sample) | ||||
Additional assessments
| · Voice examination1
| · Accelerometry | ||
· Prick test1, 2 (LIFE Child ATOPY cohort) | · Resting electrocardiography | |||
Optical coherence tomography and ophthalmoscopy2 (for a sub-sample) | · Indirect calorimetry (for a sub-sample) | |||
· Voice examination | ||||
· Electrical bioimpedance analysis | ||||
· Polysomnography (for a sub-sample) | ||||
· Eating behaviour test2
| ||||
· Optical coherence tomography and ophthalmoscopy2
| ||||
Prick test1, 2 (LIFE Child ATOPY cohort) |
Questionnaires and interview | Other assessments: |
---|---|
· Clinical history | Biological samples: |
· Questionnaires: see Table 4 | · Blood, urine and hair |
Anthropometry: | |
· Weight | |
· Height | |
· Blood pressure | |
Imaging: | |
· 3D-Bodyscan | |
Additional assessments: | |
· Voice examination |
24th– 26thweek of gestation | 34th- 36thweek of gestation | Delivery | 3rd, 6thand 12thmonth postpartum | |
---|---|---|---|---|
Anthropometry
| · Weight | · Blood pressure | · Weight | |
· Height and sitting height | ||||
· Height and sitting height | ||||
· Head, arm, leg, waist, neck and hip circumference | ||||
· Head, arm, leg, waist, neck and hip circumference | ||||
· Blood pressure | ||||
· Blood pressure | ||||
Imaging
| · 3D-Bodyscan | · 3D-Bodyscan | · 3D-Bodyscan | |
· Ultrasound data of fetal size | · Ultrasound data of fetal size | |||
Biological samples
| · Blood, urine and hair | · Blood, urine and hair | · Cord blood, biopsies of the placenta and the umbilical cord | · Blood, urine and hair |
· Breast milk | ||||
· Oral glucose tolerance test | ||||
Clinical exam
| · Family history and history of pregnancy | · Follow-up history of pregnancy | · Follow-up history of pregnancy | |
· History of delivery | ||||
· Current medications | · Current medications | · Current medications | ||
Questionnaires
| · See Table 4
| · See Table 4
| · See Table 4
| |
LIFE Child HEALTH cohort | LIFE Child OBESITY cohort | LIFE Child DEPRESSION cohort | LIFE Child BIRTH cohort | ||||
---|---|---|---|---|---|---|---|
Child participant information (to be completed by the parent and/or child) |
Parent participant information (to be completed by the parent) |
Child participant information (to be completed by the parent and/or child) |
Parent participant information (to be completed by the parent) |
Child participant information (to be completed by the parent and/or child) |
Parent participant information (to be completed by the parent) |
Pregnant woman participant information (to be completed by the pregnant woman) | |
Environment
| Sociodemography1
| Sociodemography1
| Sociodemography1
| Characteristics of family environment1
| Sociodemography1
| ||
Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| Lifestyle (sexual development, addictive behaviour, media use, sports, spare time activities, social interaction …)1
| ||
Quality of life (≥ 10 years)1
| Quality of life1
| Quality of life1
| |||||
Impact of health conditions1
| |||||||
Physical and psychological well-being, autonomy and parents, peers and social support and school environment (> 8 years)1
| Physical and psychological well-being, autonomy and parents, peers and social support and school environment (> 8 years)1
| Physical and psychological well-being, autonomy and parents, peers and social support and school environment (> 8 years)1
| |||||
Parental stress1
| |||||||
Peer relationship1
| |||||||
Parenting1
| Parenting1
| ||||||
Physical Health
| Health-related symptoms1
| Health-related symptoms1
| Health-related symptoms1
| Physical health Screening1
| |||
Alternative medicine1
| Alternative medicine1
| Alternative medicine1
| |||||
Allergies1
| Allergies1
| Allergies1
| Allergies1
| ||||
Sleep behaviour1
| Sleep behaviour1
| Sleep behaviour1
| Sleep behaviour1
| Sleep behaviour1
| Sleep behaviour1
| Sleep behaviour1
| |
Child feeding (2–13 years) Eating disorders (≥ 8 years)1
| Child feeding (2–13 years) Eating disorders (≥ 8 years)1
| Nutrition diary | |||||
Nutrition diary1
| |||||||
Pubertal status1
| |||||||
Stress regulation3 (incl. salivary cortisol samples) | |||||||
Family and School
| Self-assessed partner-attachment1
| Self-assessed partner-attachment1
| Self-assessed partner-attachment1
| ||||
Performance at school (≥ 6 years)1
| Performance at school1
| Performance at school1
| |||||
Mental Health
| Symptom Screening1
| Mental health Screening1
| |||||
Attention deficit/Hyperactivity symptoms1
| Attention deficit/Hyperactivity symptoms1
| ||||||
Perception of teasing (≥ 10 years)1
| |||||||
Life events (≥ 10 years)1
| Life events (≥ 10 years)1
| Life events, neglect, physical/emotional/sexual abuse1,2
| |||||
Mental disorders (≥ 18 years)1
| Mental disorders1
| Mental disorders (≥ 18 years)1
| Mental disorders1
| Psychiatric diagnoses2
| Mental disorders1
| ||
Illness behaviour and somatoform disorders (9 and 12 years)1
| Illness behaviour and somatoform disorders (9 and 12 years)1
| Somatic complaints1
| |||||
Anxiety symptoms1
| |||||||
Depressive symptomes1
| |||||||
Personality
| Personality (Big five concept) (≥ 18 years)1
| Personality (Big five concept)1
| Personality (Big five concept) (≥ 18 years)1
| Personality (Big five concept)1
| Personality (Big five concept)1
| ||
Risk and sensation seeking behaviour (≥ 4 years)1
| Risk and sensation seeking behaviour1
| ||||||
Control and competence beliefs (≥ 4 years)1
| Control and competence beliefs1
| ||||||
Strengths and weaknesses (≥ 3 years)1
| Strengths and weaknesses1
| ||||||
Body attitudes (≥ 8 years)1
| Body attitudes (≥ 8 years)1
| ||||||
Social competence1
| |||||||
Self perception1
| |||||||
Intelligence3
|
Participant age | Maximum volume of stored aliquots | Direct analysis |
---|---|---|
Birth
| 24 x 0.3 ml (EDTA, SG) | Not performed |
3 Month
| 4 x 0.3 ml (EDTA, SG) | Electrolytes, bilirubin, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters, vitamins, NT-pro-BNP, growth hormones |
6 Month
| 6 x 0.3 ml (EDTA, SG) | Electrolytes, bilirubin, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters, vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones |
1 – 3 years
| 8 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, sex steroids |
3 ml (Blood RNA tube) | ||
3 – 6 years
| 10 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, ferritin, sex steroids |
3 ml (Blood RNA tube) | ||
4 ml (Cell preparation tube) | ||
6 – 8 years
| 12 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, troponin T, NT- pro-BNP, growth hormones, liver function tests, renal function tests, transferrin, glucose, insulin, sex steroids |
3 ml (Blood RNA tube) | ||
4 ml (Cell preparation tube) | ||
LIFE Child OBESITY cohort and controls :
| ||
+ Oral glucose tolerance test, proinsulin, c-peptide, uric acid | ||
8 – 14 years
| 20 x 0.3 ml (EDTA, SG) | See 6 – 8 years |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
> 14 years
| 26 x 0.3 ml (EDTA, SG) | See 6 – 8 years |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
Parents
| 24 x 0.3 ml (EDTA, SG) | Not performed |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
Pregnant Women (24
th
- 26
th
week of gestation, 34
th
- 36
th
week of gestation and visits postpartum)
| 30 x 0.3 ml (EDTA, SG) | Electrolytes, protein, creatinin kinase, lipid profile, full blood count, thyroid function test, proinflammatoric cytokines, bone parameters,vitamins, allergy testing, growth hormones, liver function tests, renal function tests, transferrin,glucose, insulin, sex steroids, oral glucose tolerance test |
3 ml (Blood RNA tube) | ||
8 ml (Cell preparation tube) | ||
34th -36th week of gestation: no oral glucose tolerance test | ||
Visits postpartum: no renal function tests |