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Open Access 01.12.2013 | Review

Malaria in South America: a drug discovery perspective

verfasst von: Luiza R Cruz, Thomas Spangenberg, Marcus VG Lacerda, Timothy NC Wells

Erschienen in: Malaria Journal | Ausgabe 1/2013

Abstract

The challenge of controlling and eventually eradicating malaria means that new tools are urgently needed. South America’s role in this fight spans both ends of the research and development spectrum: both as a continent capable of discovering and developing new medicines, and also as a continent with significant numbers of malaria patients. This article reviews the contribution of groups in the South American continent to the research and development of new medicines over the last decade. Therefore, the current situation of research targeting malaria control and eradication is discussed, including endemicity, geographical distribution, treatment, drug-resistance and diagnosis. This sets the scene for a review of efforts within South America to discover and optimize compounds with anti-malarial activity.

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Electronic supplementary material

The online version of this article (doi:10.1186/1475-2875-12-168) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

LRC and TS conceived the review. LRC performed the bibliographical research, retrieved the references and drafted the manuscript. TS, MVGL and TNCW critically revised the document. All authors read and approved the final manuscript.

ACT

Artemisinin-based combinations therapy

API

Annual Parasite Incidence

G6PD

Glucose-6-phosphate dehydrogenase

RAVREDA

Amazon Network for the Surveillance of Antimalarial Drugs Resistance

WHO

World Health Organization

WRAIR

Walter Reed Army Institute of Research.

Background

Malaria is the tropical disease with the highest global mortality. In 2010, there were an estimated 216 million cases of malaria and 655,000 deaths worldwide, with children under five years and pregnant women the most vulnerable [1]. Over 81% of cases and 91% of deaths were in Africa, with the majority of the remaining being in India, Southeast Asia and South America.

South America includes 13 countries: Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela and French Guiana. Most malaria cases are concentrated in the Amazon basin, with 580,000 reported in 2010, mainly in Brazil (281,586) [2] and Colombia (115,000) [3]. In 2010 only 240 deaths were registered, 0.085% of the global total. This low number reflected a combination of factors: the higher quality of health care, and the fact that the majority of cases are Plasmodium vivax rather than Plasmodium falciparum (estimated in 70%). Plasmodium vivax mortality is often assigned to sequelae, such as haemolysis or lung inflammation, rather than the parasite itself [4, 5]. Other species of malaria have been reported. Suriname [6] and French Guiana [7] report 12% and 6% Plasmodium malariae infections respectively, although this may be an underestimate resulting from difficult diagnosis in thick-smear blood or rapid tests.

Malaria has been a long-term health issue in South America. Throughout the 20th Century, the continent underwent a rapid and disorganized development and settlement process, leading to a population migration. In the Amazon basin, with increased prospecting for minerals and agricultural projects [8, 9], work opportunities surged. This led to an increase in malaria prevalence and incidence in the 1970s and 1980s [10], a trend that is only now starting to be reversed [11].

South America, with its large biodiversity, has also played a key role in the identification of new medicines to combat malaria. The active cinchona bark, which led to the purification of quinine was first identified in Peru [12], and lapachol, the forerunner of atovaquone, also came from the Amazon basin [13]. This raises the question as to whether there are other natural products that could be useful in malaria. In addition, South America has an excellent scientific and clinical base, which can continue to support the discovery and development of new therapeutics. This review provides an overview of malaria in South America, focusing on progress in drug discovery, and highlighting critical future areas where the continent can support the malaria eradication agenda.

Malaria in South America

The endemicity of malaria can be divided into three levels: high risk, if the annual parasite incidence (API) is higher than 1% of the inhabitants; medium risk, when it is 0.1 to 1% and low risk where it is less than 0.1% [11], (see Figure 1).

Of all the South American countries, Uruguay and Chile are malaria free, with no mosquito-transmitted infections. Argentina and Paraguay are progressing towards elimination [1]. The remainder of the continent shows a broad distribution of cases, with increasing frequency towards the tropics. Brazil, has an overall API of 0.16%, reaching 0.6 to 0.7% in Amazonas and Acre [2]. On the other hand, in Colombia and Suriname 15% of the population live in areas with high transmission, and this number reaches 85% in French Guiana and Guyana where APIs of 35% have been reported locally [7].

In the rainforest region, the primary vector species that transmits Plasmodium parasites is Anopheles darlingi[14‐16], with other species such as Anopheles albitarsis, Anopheles albimanus, Anopheles aquasalis and Anopheles marajoara playing roles in transmission [17‐19]. Anopheles gambiae was imported into South America from Africa in the transatlantic slave trade but was eliminated from the continent in the first half of the 20th Century [20, 21]. Anopheles darlingi is an efficient vector, preferring humans over animals, and with a high susceptibility to Plasmodium infection [16]. Although nets are important, they are not sufficient, since many vectors have peak-biting hours before bedtime [22, 23] and in addition not all families have appropriate numbers of bed nets.

The standard treatment for uncomplicated P. falciparum malaria is artemisinin-based combination therapy (ACT), as recommended by the World Health Organization (WHO) [24], outlined in Figure 2. Chloroquine (CQ) is still effective for P. vivax in many countries. However, the Amazon Network for the Surveillance of Antimalarial Drugs Resistance (RAVREDA, Red Amazónica de Vigilancia de la Resistencia a los Antimaláricos) reported 10% resistance to chloroquine in Amazonas, Brazil [25]. Primaquine is the standard therapy for preventing relapses of P. vivax, although there are issues with compliance to the 14-day regimen and a risk of haemolysis in G6PD-deficient subjects. Studies in Brazil showed that primaquine failed to prevent relapses in 24.5% of cases [26]. Whether this is true resistance to the drug or lack of compliance is not clear. It has been suggested that an increased dosage of primaquine is required for South America [27], and Brazil and Peru have recently shifted from 14 days of 0.25 mg/kg/day to seven days of 0.5 mg/kg/day. G6PD deficiency was detected at 3% prevalence in Manaus, Brazil [28] and was predominantly the mild A^- form. In Buenaventura, Colombia, where a higher proportion of the population has African origins, prevalence of the mild A^- form is 12% [29]. An improved version of primaquine, developed originally by the Walter Reed Army Institute of Research (WRAIR), called tafenoquine, is under clinical development as a single-dose anti-relapse agent, but this is not expected to be launched before 2017. For severe falciparum malaria, most countries use parenteral quinine, although data from Africa and Asia support a shift to artesunate for injection [30], which has already been pioneered by Brazil.

Search methodology

A literature search was conducted in February and March 2012 to identify studies regarding malaria research activities in South America. The sources for published data were SciFinder Scholar®, PubMed® and LILACS®. The date of publication considered spanned from January 2000 to February 2012. The following key words were used for the database search: malaria or anti-malarial. The search list was refined by country by means of the affiliation field. All papers describing any type of drug (based on medicinal chemistry, natural products or other approaches) were selected. Only research showing either in vitro or in vivo activities of molecules was considered. In addition, the database Thomson Pharma® was screened for clinical trials’ protocols conducted within the continent. Papers regarding drug discovery research were divided into two groups: those covering natural products (divided into plant extracts and isolated substances) and those covering studies of new synthetic drug compounds.

Results

Natural products

Pharmacognosy is the study of naturally occurring molecules with medicinal properties. Plant-derived compounds have been the backbone of the anti-malarial class of drugs over the last centuries, and two emerged from South America. Quinine is the active ingredient in cinchona tree bark in Peru and was purified in 1820, becoming the first disclosed compound with known anti-malarial activity. Lapachol, belonging to the chemical class of naphthoquinones, was first isolated from Tabebuia avellanedae in 1882 and used to treat fever and malaria in the 19th Century in South America. A third natural product, artemisinin, was isolated by Chinese scientists from Artemisia annua. These natural products have served as starting points for medicinal chemistry optimization. Chloroquine was designed based on quinine, massively reducing the frequency of administration, and paving the way for a whole new generation of aminoquinolines and amino-alcohols. The chemical optimization from lapachol to atovaquone gave new molecules with more reliable oral bio-availability, allowing them to be used in prophylaxis. Modifying artemisinin to artesunate massively improved solubility (Figure 3), but has also led to the design of new improved endoperoxides such as OZ439 which is currently in Phase II trial to evaluate its efficacy and stability in malaria patients [31]. These improved molecules have been used to treat hundreds of millions of patients over the last century.

South America has a long tradition of studies of natural products based on two approaches: the biological evaluation of traditional medicines and the identification of plants (or organisms) with differences in secondary metabolism [32]. The natural products identified (pharmacognosy) are shown on Table 1. A cut-off of approximately EC₅₀ of 1 μg/mL (1 μM where the active ingredient is well characterized) was used based on the experience that almost 0.5% of chemical diversity is active at this level [33]. The structures of molecules are shown in Figure 4.

Table 1

Summary of pharmacognosy studies

Compound	Authors	Plant with the lowest IC₅₀	Type	IC₅₀(P. falciparum strain)
1	Bravo et al.[34]	Bowdichia virgiloides	Extracts	1.0 μg/mL
1	Bravo et al.[34]	Bowdichia virgiloides	Isolated substances	5 μg/mL (F32 and Indo)
-	Deharo et al.[35]	Bowdichia virgiloides	Extracts	1 μg/mL (F32)
2	da Silva Filho et al.[36]	Baccharis dracunculifolia	Extracts	13 μg/mL
2	da Silva Filho et al.[36]	Baccharis dracunculifolia	Isolated substances	0.8 μg/mL (W2 e D6)
3	de Andrade-Neto et al.[37]	Picrolemma spruce	Isolated substances	0.002 μM (K1)
4	de Andrade-Neto et al.[38]	Holostylis reniformis	Isolated substances	0.20 μM (field isolate)
-	Kayano et al.[39]	Caesalpina pluviosa	Extracts	0.59 μg/mL (3D7)
5	Rocha e Silva et al.[40]	Piper peltatum	Isolated substances	0.05–2.11 μg/mL (M1)
5	Pinto et al.[41]	Pothomorphe peltata	Isolated substances	0.67 μM (K1)
-	Garavito et al.[42]	Remijia peruviana	Extracts	0.85 μg/mL (FcB2)
-	Debenedetti et al.[43]	Buddleja globosa	Extracts	8.9 μg/mL (K1)
-	Baelmans et al.[44]	Caesalpina pluviosa	Extracts	8 μg/mL (D2)
-	Flores et al.[45]	Caesalpina pluviosa	Extracts	3.4 μg/mL
-	Flores et al.[45]	Caesalpina pluviosa	Isolated substances	Inactive (F32)
-	Ibáñez-Calero et al.[46]	Rumex obtusifolius	Isolated substances	71 μg/mL ⁱ
-	Muñoz et al.[47]	Sparanttanthelium amazonum	Extracts	2 μg/mL (F32)
-	Muñoz et al.[48]	Swietenia macrophylla	Extracts	73% ⁱⁱ
-	Muñoz et al.[49]	Tripodanthus acutifolis	Extracts	98% ⁱⁱⁱ
-	Costa et al.[50]	Montrichardia linifera	Extracts	11.7 μg/mL (W2)
-	da Silva Filho et al.[51]	Nectandra megapotamica	Extracts	28 μg/mL
-	da Silva Filho et al.[51]	Nectandra megapotamica	Isolated substances	3.8 μg/mL (D6)
-	de Andrade-Neto et al.[52]	Bidens pilosa	Extracts	3.1 μg/mL (D6)
-	de Andrade-Neto et al.[53]	Remijia ferruginea	Extracts	48% ^iv
-	de Mesquita et al.[54]	Matayba guianensis	Isolated substances	2.5 μg/mL (FcB1)
-	Dolabela et al.[55]	Esenbeckia febrifuga	Extracts	15.5 μg/mL
-	Dolabela et al.[55]	Esenbeckia febrifuga	Isolated substances	75.3 μg/mL (W2)
-	Estevam et al.[56]	Ouratea nitida	Extracts	51.04% ^v
-	Fischer et al.[57]	Xylopia emarginata	Extracts	3.3 μg/mL (PA)
-	Morais et al.[58]	Pentacalia desiderabilis	Isolated substances	7.82 μg/mL (K1)
-	Oliveira et al.[59]	Bidens pilosa	Extracts	38% ^vi
-	Sá et al.[60]	Physalis angulata	Isolated substances	2.2 μM (W2)
-	Uchôa et al.[61]	Cecropia pachystachya	Extracts	66% ⁴
-	Uchôa et al.[61]	Cecropia pachystachya	Isolated substances	58% ^vii
-	Loyola et al.[62]	Azorella compacta	Isolated substances	60% ^viii
-	Pabón et al.[63]	Solanum nudum	Isolated substances	21 μM (FcB2)
-	Céline et al.[64]	Siparuna aspera	Extracts	6.4 μg/mL (FCR-3)
-	Ruiz et al.[65]	Minquartia guianensis	Extracts	4.2 μg/mL (FCR-3)

ⁱ Inhibition of biocrystallization of ferriprotoporphyrin IX.

ⁱⁱ Percentage of inhibition of parasite growing (dose: 250 mg/kg).

ⁱⁱⁱ Percentage of inhibition of parasite growing (at 10 μg/mL).

^iv Reduction of parasitaemia (dose: 1000 mg/kg).

^v Activity tested against Plasmodium berghei in mice (dose: 1000 mg/kg).

^vi Reduction of parasitaemia at day 5 (dose: 250 mg/kg).

^vii Percentage inhibition of parasitaemia in relation to untreated infected mice on day 8 after malaria infection (doses: 250 and 15 mg/kg respectively).

^viii Activity in mice measured by the growth of inhibition (dose: 10 mg/kg/day).

Thus, only five purified compounds (Figure 4) have been identified from these efforts. Studies of Bowdichia virgiloides, a plant used by the Tacana indigenous group as a traditional medicine for the relief of high fever, produced alkaloid 1 (ormosanine), having an EC₅₀ = 5 μg/mL against F32. In vivo, the extract showed 51% suppression of parasites in mice at 100 mg/kg, but was toxic at 250 mg/kg. Baccharis dracunculifolia is broadly used in traditional medicine in Brazil, in inflammatory and gastrointestinal diseases. Although the total extract was inactive, the isolated the triterpenoid 2 showed anti-malarial and anti-leishmanial activity. In vitro screening of substances isolated from the Brazilian folk medicines identified neosergeolide 3, from Picrolemma spruce, which inhibits K1 with an impressive EC₅₀ = 2 nM; and, the aryltetralone 4 from Holostylis reniformis, with an EC₅₀ = 20 nM; both are claimed to have good therapeutic window against hepatocytes. Further testing of these compounds would be needed to assess their strengths and weaknesses. The 4-nerolidylcatechol 5 was isolated from another traditional Amazonian treatment of malaria, Piper peltatum and shown to have an EC₅₀ between 50 and 830 ng/ml. Catechol 5 was also independently isolated from Pothomorphe peltata as shown in Table 1.

Medicinal chemistry

Medicinal chemistry approaches start from the knowledge of a structure combined with biological activity. Such starting points can be found from screening efforts (for example, pharmaceutical diversity or natural products against a biochemical target or whole cell), de novo design or from a published active, which can then act as a starting point for optimization. The molecules that have been identified from various sources against malaria with relevant endpoints and published within the South American medicinal chemistry community are summarized in Table 2, and in addition, their structures are presented in Figure 5. Studies characterizing the spectroscopy of previously described molecules, or studies on marketed anti-malarials have not been included.

Table 2

Summary of medicinal chemistry studies

Compound	Authors	Target	Chemical class	Lowest IC₅₀(strain)
6	Sperandeo and Brun [[66]]	-	Pyrazolylnaphthoquinones, 5-aminoisoxazole	0.11 μg/mL (K1)
7	Silva et al.[67]	-	1,4-naphthoquinones	0.03 μM (FcB1)
8	Charris et al.[68]	β-haematin	Thieno(2,3-b)quinoline	74.42%¹
9	Cunico et al.[69]	Aspartyl protease	Hydroxyethylpiperazines	4.6 μM (3D7)
10	Cunico et al.[70]	Aspartyl protease	Hydroxyethyylpiperazines	5.1 μg/mL (W2)
11	Gnoatto et al.[71]	β-haematin	Piperazine, 3-acetylursolic acid	0.08 μM (FcB1)
12	de Sá et al.[72]		Betulinic acid	5.99 μM (W2)
13	Pinheiro et al.[73]	-	Sesquiterpenes	0.05 ng/mL (P. falciparum mefloquine resistant)
14	Barbosa et al.[74]	-	Ozonides	13.6 μg/mL
15	Oliveira et al.[75]	Cysteine proteases	Semicarbazone, Thiosemicarbazone	7.2 μM (W2)
16	Camacho et al.[76]	β-haematin	Benzimidazole-5-carbohydrazides	8.43 μM
17	Corrales et al.[77]	Hypoxanthine-guanine phosphoribosyltransferase	6-thiopurine Steroids	82%¹
18	Barazarte et al.[78]	β-haematin	Pyrazolo and pyrimido benzothiazine dioxide	92.32%²
19	Barazarte et al.[79]	β-haematin	Benzothiazines	78.17%²
20	Vellasco Junior et al.[80]	Aspartyl protease	Thioetherhydroxyethyl, Sulfonamides	15 μM (W2)
21	Dominguez et al.[81]	β-haematin	Chlorovinyl sulfones	0.025 μM (W2)
22	Dominguez et al.[82]	β-haematin	Sulfonamide chalcones	0.48 μM (W2)
23	León et al.[83]	Glucose metabolism β-haematin	Sulfonylureas	1.2 μM (W2)
24	Arancibia et al.[84]	β-haematin	Rhenium bioorganometalics, Aminoquinoline	0.048 μM (3D7)
25	Rojas Ruiz et al.[85]	β-haematin	Aminoquinolines, thiazolidinone	0.25 μM (3D7)
26	Charris et al.[86]	β-haematin	E-2-quinolinylbenzo-cycloalcanones	90%³
27	Vashist et al.[87]	β-haematin	Quinolone, 6-thiopurine	inactive
28	de Souza et al.[88]	β-haematin	4-aminoquinolines, platinum (II) complexes	84 %⁴
29	Cunico et al.[89]	β-haematin	4-aminoquinolines	1.39 μg/mL (W2)
30	Rodrigues et al.[90]	β-haematin	Quinoline	Active
31	Domínguez et al.[91]	Cysteine protease falcipain	Quinolinyl chalcones	19 μM (FcB1)
32	Ferrer et al.[92]	β-haematin	Chloroquinolines	94.93%³
33	Navarro et al.[93]	β-haematin	Gold-chloroquine complexes	−⁵
34	Domínguez et al.[94]	Cysteine protease β-haematin	Phenylurenyl chalcones	1.76 μM
35	de Andrade-Neto et al.[95]	-	Naphthoquinones, Phenazines	1.67 μM (W2)
36	Hilário et al.[96]	-	3-alkylpyridines alkaloids	<3.38 μM (W2)
37	Rodrigues et al.[97]	β-haematin	Bisquinoline	56.76 %²

¹ Percentage of inhibition of parasite growth (P. berghei in mice) at day 9 (dose: 10 mg/kg).

² Inhibition of globin proteolysis (IGP) expressed as percentage.

³ Inhibition of β-haematin synthesis (IβHS) expressed as percentage.

⁴ Inhibition of parasite multiplication on days (dose: 25 mg/kg).

⁵ Activity expressed as IC₅₀(CQDP)/IC₅₀(complex).

Those compounds having EC₅₀ values less than 1 μg/ml (with a square box around) are discussed further as this is the typical potency cut-off required for “Validated Hits” – the starting points for drug discovery projects, as considered by the Medicines for Malaria Venture [98, 99].

New pyrazolylnaphthoquinones (heterocyclic naphthoquinones, building on the atovaquone template bearing 3-aminopyrazole rings) and 5-aminoisoxazole analogues showed activity against P. falciparum, Trypanosoma cruzi and Trypanosoma brucei. The 5-aminoisoxazole analogue 6 showed an EC₅₀ of 110 ng/mL and an independent naphthoquinone, 7, demonstrated an EC₅₀ of 30 nM against FcB1. A novel piperazinyl/steroidal analogue, 11, also inhibited FcB1 with an EC₅₀ of 0.08 μM. Pinheiro et al. used a multivariate and quantum mechanical method to analyse 15 dihydroartemisinin derivatives and the most potent compound, 13, showed an EC₅₀ of 0.05 ng/mL, over 10-fold more potent than the reported values for dihydroartemisinin. Two papers describe chalcone derivatives: the electrophilic chloro-vinyl sulphone 21 showed an EC₅₀ of 0.025 μM against W2 and the sulphonamide chalcone 22 showed an EC₅₀ of 0.48 μM. Finally, approaches to aminoquinolines identified the derivative 24 with an EC₅₀ of 48 nM, against the 3D7 strain; and new heterocyclic hybrids based on the chloroquine and thiazolidinone scaffolds such as 25 have an EC₅₀ of 0.25 μM.

Discussion

In South America, the morbidity and mortality due to malaria is much less significant than in Africa. However, the continent has historically been the source of two of the major classes of drugs against malaria, and the combination of both biodiversity and skilled medicinal chemists could position the continent in a leading position in the search for the new medicines needed for malaria eradication. The current biggest threat in the fight against malaria is the emerging resistance to artemisinin derivatives [100, 101]. Artemisinin derivatives within ACT are the most widely used anti-malarials. Even though there has been a concerted attempt to protect them against resistance by banning artesunate monotherapy for uncomplicated disease, the first signs of artemisinin resistance or insensitivity have been described in Cambodia [102] and more recently in Thailand [103]. There is a great need for new combination therapy, replacing the three days’ dosing of ACT with a single dose that also prevents transmission and relapse (in the case of P. vivax or Plasmodium ovale) [104].

Pharmacognosy has continued to identify new active structures [105]. However, the progress in bringing forward new medicines from these structures and extracts is extremely difficult. Where such molecules are reported to have interesting properties from observational studies, then it is important to confirm these observations in carefully controlled human clinical studies [106]. Also, it is possible that the active principle is a metabolite from the original extract, and so analysis of plasma samples is also important in understanding and identifying the active species [33]. Secondary metabolites are usually thought to play a key role in protection against predators, and therefore could be expected to be cytotoxic. Screening for activity in early safety assays is therefore of paramount concern here. Ultimately the goal of such experiments is to identify new starting points for medicines, similar to the way that quinine and lapachol opened up new fields in previous centuries. Such approaches require long-term commitment, and hence the need to verify the original clinical observations.

The other approach to discover new drugs is to use medicinal chemistry, either with scaffolds already known to be effective against the parasite, or a target-based approach based on structural biology. The results of this survey show that molecules coming from South American programmes are able to demonstrate innovative and active new structures. However over the last five years, the bar has been raised and a new challenge has been set as a result of the success of phenotypic screening. With over 20,000 structures of compounds active against the parasite deposited in the public domain [107, 108], it is important to benchmark the successes found in South America against these results. Clearly the prize no longer goes to compounds that simply kill the parasite in vitro, but to molecules that have good properties supporting excellent oral administration in patients, or perhaps equal artemisinins in speed of killing parasites [109], or have a very low propensity to resistance generation [110]. In the light of the malaria eradication agenda, it will be important also to know how these new molecules work in the different stages of the parasite lifecycle [111]. A molecule that could be shown to inhibit the dormant liver stages of P. vivax would clearly stand out from the crowd [112]. All of the tools are available to enable South American anti-malarial drug research to make these steps forward over the next five years, the challenge will be to put these together, and focus the agenda to the needs of the South American community.

Conclusion

Malaria continues to be a health issue, particularly P. vivax in the Amazon basin, and P. falciparum and mixed infections in northern countries. The natural diversity along with the indigenous folk medicines allows a great potential in the treatment and identification of new anti-malarial drugs, as happened with the South American compounds, lapachol and quinine. New molecules are being identified but their optimization for in vivo activity has been slow, arguing that more resource needs to be focused in these areas. In addition, the new assays for transmission and relapse of dormant liver stages need to be put into routine practice. If all this is put together, then South America can again play a leading role in the discovery of the next generation of therapeutics against malaria.

Acknowledgements

We acknowledge the Medicines for Malaria Venture, especially Jeremy Burrows for critical comments on the manuscript. The authors are also grateful to Carlos Morel and Simon Campbell. MVGL is a level 2 Fellow from the National Council for Scientific and Technological Development (CNPq).

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

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Titel: Malaria in South America: a drug discovery perspective
verfasst von: Luiza R Cruz
Thomas Spangenberg
Marcus VG Lacerda
Timothy NC Wells
Publikationsdatum: 01.12.2013
Verlag: BioMed Central
Erschienen in: Malaria Journal / Ausgabe 1/2013
Elektronische ISSN: 1475-2875
DOI: https://doi.org/10.1186/1475-2875-12-168

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Abstract

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Competing interests

Authors’ contributions

Background

Malaria in South America

Search methodology

Results

Natural products

Medicinal chemistry

Discussion

Conclusion

Acknowledgements

Competing interests

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Authors’ original submitted files for images

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