Background
Methods
Brain tissue specimens
Histopathological examination
Parameters | Histological characterization | Score |
---|---|---|
Sequestration of PRBCs | None | 0 |
≤ 30%/HPF | 1 | |
31-50%/HPF | 2 | |
51-70%/HPF | 3 | |
71-90%/HPF | 4 | |
> 91%/HPF | 5 | |
Ring haemorrhage | None | 0 |
≤ 10 areas/slide | 1 | |
11-20 areas/slide | 2 | |
21-30 areas/slide | 3 | |
Durck’s granuloma | None | 0 |
≤ 2 areas/slide | 1 | |
> 2 areas/slide | 2 | |
Total Score: (5) + (3) + (2) = 10
|
Immunohistochemistry for NF-κB p65 and cleaved caspase-3
Positive and negative controls
Evaluation of NF-κB p65 and cleaved caspase-3 immunostaining
Statistical analysis
Results
Clinical data of fatal CM and NCM cases
Code no. | Group | Age (Year) | Sex | Hb†(g/dl) | WBC†(μl) | Time to death (hr) | Admission parasite count/μl | Last parasite count/μl | Other complications |
---|---|---|---|---|---|---|---|---|---|
A001 | CM | 3 | F | 12 | ND | 12 | 677,040 | 677,040 | Anaemia, Jaundice, Pneumonia, Shock, PE |
A002 | CM | 18 | M | 8 | 28,200 | 4 | 29,900 | 29,900 | Anaemia, Jaundice, PE |
A003 | CM | 15 | M | 12 | 18,350 | 0.5 | 190,000 | 190,000 | Jaundice, Pneumonia, DIC, PE |
A004 | CM | 28 | M | 7.3 | 32,500 | 96 | 20,460 | 3,410 | Anaemia, Jaundice, Pneumonia, AKI |
A005 | CM | 35 | F | 14.6 | 6,941 | 144 | 52,780 | 81,200 | AKI |
A006 | CM | 41 | M | 14.3 | 23,250 | 13 | 521,730 | 521,730 | Pneumonia, Shock |
A007 | CM | 18 | M | 13.2 | 4,350 | 48 | 2,494,940 | 702,800 | Jaundice, AKI, Hypoglycemia |
A008 | CM | 13 | M | 10.7 | 6,600 | 96 | 313,900 | 64,500 | Anaemia, Jaundice, Pneumonia, Hypoglycemia, PE |
A009 | CM | 8 | F | 9.5 | 27,700 | 8 | 780,700 | 1,122,806 | Anaemia, Jaundice, AKI, Acidosis, DIC, PE |
A101 | NCM | 65 | M | 11.4 | 9,312 | 120 | 150 | 0 | Pneumonia, PE |
A102 | NCM | 38 | M | 6.8 | 4,800 | 144 | 96 | 0 | Metabolic acidosis, Pneumonia, PE |
Histopathological observations in the brain of fatal CM and NCM patients
Histopathological changes | |||||
---|---|---|---|---|---|
Code no. | Group | aPRBC sequestration | bRing haemorrhage | cDurck’s granuloma | †Total score |
A001 | CM | 5 | 1 | 0 |
6
|
A002 | CM | 5 | 3 | 0 |
8
|
A003 | CM | 3 | 0 | 0 |
3
|
A004 | CM | 1 | 3 | 1 |
5
|
A005 | CM | 1 | 1 | 0 |
2
|
A006 | CM | 5 | 1 | 0 |
6
|
A007 | CM | 5 | 0 | 0 |
5
|
A008 | CM | 1 | 0 | 2 |
3
|
A009 | CM | 1 | 0 | 1 |
2
|
A101 | NCM | 0 | 0 | 0 |
0
|
A102 | NCM | 0 | 0 | 0 |
0
|