Background
Methods
Study site
Experimental huts
Biological materials
Sleepers and mosquito collection
-
Deterrence rate: percentage of reduction in the number of mosquitoes caught in treated hut relative to the number caught in the control hut;
-
Exophily rate: percentage of mosquitoes that have escaped the hut and have taken refuge in the veranda trap divided by the total number of mosquitoes collected in the hut;
-
Blood-feeding rate: percentage of blood fed mosquitoes collected divided by the total of mosquitoes collected in verandah and hut;
-
Immediate mortality: percentage of dead mosquitoes collected in the morning compared to total mosquitoes collected in the hut;
-
Overall mortality: general mortality: immediate mortality + delayed mortality recorded after 24 h.
Collection of qualitative data
Statistical analysis
Ethical approval
Results
Attractiveness of huts before treatment
Results of collections after insecticide treatment
Deterrency and induced exophily
Treatments | Month | Number of females caught | Deterrency (%) | Exophily (%) | Blood feeding (%) | Immediate mortality (%) | Overall mortality (%) | ||||
---|---|---|---|---|---|---|---|---|---|---|---|
Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | ||||
Control
|
1
|
80
|
-
|
12,5
a
|
-
|
31,25
a
|
-
|
0
a
|
-
|
3,7
a
|
-
|
2 | 101 | - | 19,80a | - | 29,70a | - | 0a | - | 2,97a | ||
Bend
|
1
|
70
|
12,5
|
28,6
a
|
[14,49-42,30]
|
20
a
|
[12,13-31,85]
|
57,14
c
|
[48,65-69,86]
|
92,85
c
|
[85,76-99,65]
|
2 | 80 | 20,79 | 22,50a | [9,80-28,85] | 12,50b | [3,05-19,05] | 25bc | [10,93-41,82] | 68,75c | [56,42-81,80] | |
Delta
|
1
|
55
|
31,25
|
45,4
b
|
[25,31-74,95]
|
18,18
a
|
[6,28-33,91]
|
32,72
b
|
[18,40-51,46]
|
72,72
b
|
[58,79-84,06]
|
2 | 76 | 24,75 | 26,31a | [15,86-37,36] | 23,68a | [7,04-50,63] | 15,78b | [6,81-29,32] | 31,57b | [22,81-40,33] | |
A-Cyper
|
1
|
61
|
23,8
|
42,6
b
| [24,62-61,36] |
19,67
a
|
[6,65-33,45]
|
27,86
b
|
[16,29-44,95]
|
77,04
b
|
[59,85-95,52]
|
2 | 89 | 11,88 | 22,47a | [15,18-31,77] | 13,48a | [6,88-21,50] | 19,10b | [10,98-29,85] | 39,32b | [34,33-44,32] | |
Féni
|
1
|
85
|
0
|
24,7
a
|
[13,16-39,70]
|
9,41
b
|
[3,03-17,35]
|
91,76
d
|
[84,30-96,87]
|
100
c
|
[100-100]
|
2 | 95 | 5,94 | 22,10a | [14,20-28,93] | 12,63b | [4,14-20,38] | 40cd | [30,73-52,55] | 73,68c | [64,41-82,95] | |
Chlor- Delta
|
1
|
85
|
0
|
17,6
a
|
[8,95-25,94]
|
10,58
b
|
[3,01-22,59]
|
95,3
d
|
[89,20-101,36]
|
100
c
|
[100-100]
|
2 | 70 | 30,69 | 25,71a | [15,05-39,97] | 11,42b | [4,02-17,35] | 44,28d | [27,12-74,50] | 78,57c | [62,06-95,00] |
Treatments | Number of females Catches | Deterrency (%) | Exophily (%) | Blood feeding (%) | Immediate mortality (%) | Overall mortality (%) | ||||
---|---|---|---|---|---|---|---|---|---|---|
Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | |||
Control
| 975 | - | 30,76a | - | 19,48a | - | 0,30a | - | 2,56a | - |
Bend
| 550 | 43,58 | 27.27a | [23,00-32,53] | 16,36b | [14,22-19,00] | 19,09c | [15,84-23,02] | 38,36c | [35,46-41,35] |
Delta
| 525 | 46,15 | 33,33a | [28,67-38,33] | 14,28b | [9,58-20,07] | 8,5b | [7,07-10,10] | 20,95b | [17,74-23,79] |
α-Cyper
| 601 | 38,35 | 33,44a | [31,74-35,72] | 16,63b | [9,24-24,02] | 11,81b | [5,57-18,05] | 24,29b | [14,57-33,72] |
Féni
| 805 | 17,43 | 21,73a | [18,41-24,63] | 14,90b | [13,91-15,77] | 31,18e | [27,80-34,13] | 53,41d | [50,14-56,37] |
Chlor- Delta
| 850 | 12,82 | 27,05a | [25,10-29,01] | 14,11b | [12,79-15,45] | 23,64d | [22,26-24,86] | 52,11d | [49,22-55,21] |
Treatments | Month | Number of females caught | Exophily (%) | Blood feeding (%) | Immediate mortality (%) | Overall mortality (%) | ||||
---|---|---|---|---|---|---|---|---|---|---|
Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | Rate | Conf Lim | |||
1
|
62
|
16,12
a
|
-
|
32,25
a
|
-
|
0
a
|
-
|
3,22
a
|
-
| |
2 | 73 | 27,39a | - | 13,69a | - | 0a | - | 2,73a | - | |
Control
|
3
|
73
|
6,84
a
|
-
|
10,95
a
|
-
|
0
a
|
-
|
4,10
a
|
-
|
4 | 49 | 42,85a | - | 10,20a | - | 0a | - | 6,12a | - | |
1
|
60
|
25
b
|
[21,92-28,16]
|
25
b
|
[21,92-28,16]
|
50
e
|
[43,86-56,31]
|
81,66
d
|
[76,23-87,20]
| |
2 | 48 | 33,33b | [28,28-38,31] | 10,41a | [2,43-18,19] | 72,91d | [67,44-78,50] | 81,25d | [68,78-94,14] | |
Bend
|
3
|
72
|
51,38
e
|
[45,40-57,37]
|
8,33
a
|
[8,33-8,33]
|
69,44
c
|
[63,47-75,40]
|
77,77
c
|
[71,79-83,76]
|
4 | 46 | 63,04d | [54,68-71,42] | 4,34a | [-5,11-14,01] | 10,86b | [1,82-19,85] | 47,82d | [35,72-60,11] | |
Delta
|
1
|
60
|
38,33
c
|
[29,55-47,26]
|
16,66
d
|
[7,31-19,24]
|
25
b
|
[21,92-28,16]
|
66,66
b
|
[62,45-70,95]
|
2 | 52 | 71,15d | [68,80-73,47] | 9,61a | [1,57-17,60] | 7,69b | [-0,9385-16,4] | 67,30c | [59,88-74,76] | |
3
|
63
|
23,80
b
|
[23,81-23,81]
|
6,34
a
|
[-0,48-13,17]
|
6,34
a
|
[-0,48-13,17]
|
22,22
b
|
[15,39-29,05]
| |
4 | 32 | 46,87ab | [40,44-53,49] | 9,37a | [8,09-10,70] | 3,12a | [-10,00-16,07] | 28,12b | [24,26-32,09] | |
1
|
60
|
35
c
|
[30,70-39,42]
|
18,33
c
|
[10,71-26,02]
|
26,66
b
|
[22,15-31,11]
|
78,33
c
|
[71,68-81,75]
| |
2 | 82 | 59,75c | [57,66-61,82] | 12,19a | [7,60-16,73] | 39,02c | [34,39-43,65] | 60,97b | [56,34-65,60] | |
α-Cyper
|
3
|
78
|
42,30
d
|
[42,31-42,31]
|
5,12
a
|
[-0,37-10,63]
|
3,84
a
|
[3,85-3,85]
|
24,35
b
|
[18,85-29,86]
|
4 | 34 | 55,88bcd | [38,81-73,31] | 5,88a | [-6,97-19,10] | 29,41c | [14,60-44,47] | 35,29c | [31,01-39,70] | |
1
|
70
|
27,14
b
|
[22,70-31,53]
|
24,28
b
|
[18,65-29,90]
|
42,85
c
|
[40,28-45,47]
|
85,71
d
|
[80,54-90,96]
| |
2 | 43 | 67,44d | [58,43-76,49] | 11,62a | [1,95-21,23] | 74,4d | [65,25-83,63] | 83,72d | [75,62-91,99] | |
Féni
|
3
|
54
|
22,22
b
|
[19,18-25,34]
|
3,7
b
|
[-4,31-11,73]
|
68,51
c
|
[56,65-80,65]
|
90,74
d
|
[76,47-105,37]
|
4 | 45 | 51,11bc | [41,55-60,66] | 4,44a | [-5,11-14,01] | 71,11d | [61,55-80,66] | 77,77f | [65,99-85,11] | |
1
|
80
|
25
b
|
[20,89-29,07]
|
26,25
b
|
[24,84-27,68]
|
46,25
d
| 41,64-50,86] |
81,25
d
|
[72,79-89,80]
| |
2 | 68 | 58,82c | [53,40-64,26] | 8,82a | [8,28-9,40] | 70,58d | [66,09-75,15] | 82,35d | [76,92-90,67] | |
Chlor- Delta
|
3
|
67
|
35,82
c
|
[33,56-38,09]
|
4,47
b
|
[4,19-4,77]
|
61,19
b
|
[55,50-66,89]
|
88,05
d
|
[81,93-94,21]
|
4 | 49 | 57,14cd | [45,17-60,66] | 2,04b | [-6,88-11,05] | 28,57c | [20,57-36,53] | 63,26e | [60,07-66,41] |