Background
Multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) are characterised by infiltration to the central nervous system (CNS) of autoantigen-specific T cells, and recruitment of myeloid cells, including dendritic cells (DC) and macrophages, leading to development of inflammatory lesions, demyelination and axonal damage [
1,
2]. Recognition of usually myelin epitopes is required for initiation and progression of EAE [
1]; however, the mechanisms underlying initiation and control of T cell responses in CNS inflammation are less well understood. Two CD4
+ T cell subtypes, the IFN-γ secreting T helper (Th)1 and IL-17 secreting Th17 cells, have been shown to play a pathogenic role in EAE [
1,
3], although neither of the nominal cytokines is absolutely required [
4,
5].
Activation of CD4
+ T cells is a multistep process initiated by the appropriate binding of the T-cell receptor to its cognate antigenic peptide presented by major histocompatibility complex (MHC) class II molecules and subsequent stimulation by co-stimulatory molecules such as CD80 and CD86 on the antigen presenting cells (APC). During the activation process, both T cell and APC produce cytokines that shape the immune response. Cytokines that direct Th1 responses include IL-12 and IL-18, while Th17 are directed by transforming growth factor (TGF)-β, IL-1β, IL-6, and IL-23. Regulatory T cells, that exert an anti-inflammatory effect, are directed by TGF-β in the absence of other Th17-inducing signals, or by IL-10 [
1].
The APC responsible for T cell re-activation within the CNS remain to be precisely identified. DC, infiltrating macrophages, B cells and CNS-resident microglia can all express MHC class II and co-stimulatory molecules that are required for the initiation and progression of EAE [
1]. Of these, DC are the most generally accepted as ‘professional’ APC that can induce an immune response. The fact that DC in the uninflamed CNS are located outside the parenchyma, in perivascular locations [
6,
7], has contributed to a model whereby T cells receive pro-infiltratory signals through interaction with DC in post-capillary perivascular space [
8]. However it remains unclear whether parenchymally located APC, within the CNS, can provide an equivalent signal for infiltrating T cells. Results from a number of studies led to the conclusion that microglia were not as effective APC as DC or macrophages [
1,
9,
10].
We described a subset of CD11c
+ microglia that were induced in cuprizone-demyelinated or injury-reactive CNS. These cells shared with CD11c
− microglia the characteristic of an intermediate level of expression of CD45 that discriminates microglia from blood-infiltrating cells [
11]. Phenotypically similar cells were induced by experimental synaptic degeneration in the hippocampus dentate gyrus [
12]. Importantly, the cuprizone-induced CD11c
+ microglia were potent APC for a T cell proliferative response [
11].
In this study, we demonstrate that infiltrating CD11c+ cells that include DC, and CNS-resident CD11c+ microglia sorted from the CNS during EAE and studied directly ex vivo, express similar levels of the MHC class I and II molecules, CD80 and CD86. We furthermore show that both populations are equivalently capable of inducing an antigen-specific proliferative response from primed T cells. Whereas infiltrating CD11c+ cells expressed all of the Th1- and Th17-inducing cytokines tested, CD11c+ microglia only expressed TGF-β and a low level of IL-1β but not IL-6, IL-12p35 or IL-23p19. Interestingly, in contrast to CD11c+ microglia, CD11c− microglia did express IL-6, IL-12p35 and IL-23p19. Correspondingly, T cell cytokine responses elicited by these three CNS APC populations differed in magnitude and cytokine profile. CD11c+ microglia were weak inducers of Th1 and Th17 cytokines, whereas infiltrating CD11c+ cells more strongly induced both Th1 and Th17 cytokines. CNS-resident CD11c− microglia induced very weak proliferative and cytokine responses. Thus, the inflamed CNS contains APC subpopulations with distinct and possibly complementary capability.
Methods
Mice
Female C57BL/6j bom (B6) mice aged 6 to 8 weeks were obtained from Taconic Europe A/S, (Lille Skensved, Denmark) and maintained in the Biomedical Laboratory, University of Southern Denmark (Odense). All experiments were approved by the Danish Ethical Animal Care Committee (approval number 2009/561-1724 and 2012-15-2934-00110).
Active induction of experimental autoimmune encephalomyelitis
Seven- to eight-week-old female mice were immunised by injecting subcutaneously 100 μl of an emulsion containing 100 μg of myelin oligodendrocyte glycoprotein (MOG)p35–55 (Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense) in incomplete freunds adjuvant (DIFCO, Alberstslund, Denmark) supplemented with 400 μg H37Ra Mycobacterium tuberculosis (DIFCO). Bordetella pertussis toxin (300 ng; Sigma-Aldrich, Brøndby, Denmark) in 200 μl of PBS was injected intraperitoneally at day 0 and day 2. Animals were monitored daily from day 5 and scored on a 6-point scale as follows: 0, no symptoms; 0.5, partial loss of tail tonus; 1, complete loss of tail tonus; 2, difficulty to right, 3, paresis in one or both hind legs; 4, paralysis in one or both hind legs; 5, front limb paresis; 6, moribund. About 75% of the mice showed symptoms of EAE. Severe EAE usually developed 14 to 18 days after immunisation and was defined as a score of 3 to 5.
Isolation of central nervous system antigen presenting cells, spleen dendritic cells and T cells
To isolate mononuclear cells from the CNS, mice were anaesthetised with 0.2 mg pentobarbital (200 mg/ml; Glostrup Apotek, Glostrup, Denmark) per gram of mouse and intracardially perfused with ice-cold PBS when they showed symptoms of severe EAE. CNS tissue was collected and a single cell suspension was generated by forcing through a 70 μm cell strainer (BD Biosciences, Albertslund, Denmark). Mononuclear cells were collected after centrifugation on 37% Percoll (GE Healthcare Bio-sciences AB, Brøndby, Denmark). They were then first incubated with anti-Fc receptor (Clone 2.4G2; 1 μg/ml; BD Pharmingen,Albertslund, Denmark) and Syrian hamster IgG (50 μg/ml; Jackson Immuno Research Laboratories Inc., Skanderborg, Denmark) in PBS 2% fetal bovine serum (FBS), then with anti-CD45, anti-CD11b and anti-CD11c antibodies (Table
1) in PBS 2% FBS. Cell populations were gated based on isotype control antibodies as CD45
dim CD11b
+ CD11c
− (CD11c
− microglia), CD45
dim CD11b
+ CD11c
+ (CD11c
+ microglia) and CD45
high CD11c
+ and were sorted on a FACSVantage™ or FACSAria™ III cell sorter (BD Biosciences).
Table 1
Antibodies used in this study
PerCP-Cy5.5 -anti-mouse CD11b | M1/70 | Biolegend |
Biotinylated- anti-mouse CD11c | HL3 | BD Pharmingen |
FITC- anti-mouse CD80 (B7.1) | 16-10A1 | Biolegend |
FITC- anti-mouse CD86 (B7.2) | GL1 | Biolegend |
FITC- anti mouse I-Ab (MHC class II) | 25-9-17 | Biolegend |
FITC- anti mouse H2-Kb (MHC Class I) | AF6-88.5 | Biolegend |
PE- anti-mouse CD45 | 30-F11 | Biolegend |
To isolate spleen DC, naive B6 mice were killed by cervical dislocation. Spleens were removed and dissociated by forcing through a 70 μm cell strainer. Erythrocytes were lysed with 0.83% NH4Cl. DC were isolated by magnetic separation using CD11c nanobeads (Stemcell Technologies, Grenoble, France). Final purity was 85 to 90%.
T cells for proliferation assay were isolated from mice with severe EAE. Lymph nodes were dissociated by forcing through a 70 μm cell strainer and CD4 T cells were isolated by magnetic separation using a CD4 negative selection kit (Stemcell Technologies). Final purity was 90 to 95% CD4 cells.
Flow cytometry
Cells were first incubated with anti-Fc receptor and Syrian hamster IgG in PBS, 2% FBS, 0.1% sodium azide then with cell surface marker specific antibodies in PBS, 2% FBS, 0.1% sodium azide as specified in Table
1. Cells stained with biotinylated antibodies were incubated with streptavidin-fluorochrome conjugates (Biolegend, Copenhagen, Denmark). We used the same gating strategy as for fluorescence-activated cell sorting (FACS) and appropriate isotype control antibodies. Data were collected on a FACSCalibur™ or LSRII™ flow cytometer (BD Biosciences) and analyzed using FACSDiva™ software version 6.1.2 (BD Biosciences).
Proliferation assay
Primed T cells were stained with 2 μM CFSE (Sigma-Aldrich). T cells (2 × 105) from each stained preparation were cultured with CNS APC (5 × 104) with or without 25 μg/ml MOGp35–55 peptide in RPMI Glutamax (Invitrogen, Nærum, Denmark) supplemented with 10% FBS, 50 U/ml penicillin-streptomycin (Invitrogen) and 50 μM β-mercaptoethanol (Invitrogen). Supernatants were collected for cytokine assay at day 1. Three days after initiation of the co-culture, cells were harvested, stained with an anti-CD4 antibody and CFSE dilution was analyzed by flow cytometry.
Cytokine assay
IL-17A and IFN-γ were measured in the culture supernatants from the APC-T-cell cultures using a BD cytometry bead array (CBA) Th1/Th2/Th17 CBA kit following the manufacturer’s instructions (BD Biosciences).
Sorted CD11c+ microglia, CD11c− microglia and CD45high CD11c+ cells were placed in RLT buffer (Qiagen, Copenhagen, Denmark) and total RNA was extracted using RNeasy columns as per manufacturer’s protocol (Qiagen). Reverse transcription was performed with M-MLV reverse transcriptase (Invitrogen) according to the manufacturer’s protocol.
Quantitative real-time PCR (qRT-PCR) was performed with 1 μl cDNA in a 25 μl reaction volume containing Maxima® Probe/ROX qPCR Master mix (Fermentas, St Leon-rot, Germany), forward and reverse primers (800 nM; from TAG Copenhagen A/S, Frederiksberg, Denmark) and probe (200 nM; Applied Biosystems, Nærum, Denmark, and TAG Copenhagen A/S). Primer and probe sequences were as follows: CCR2 - Forward: GAAGTATCCAAGAGCTTGATGAAGG; Reverse: CAAGCTCCAATTTGCTTCACAC; Probe: CCACCACACCGTATGACT. TGFβ - Forward: TGACGTCACTGGAGTTGTACGG; Reverse: GGTTCATGTCATGGATGGTGC; Probe: TTCAGCGCTCACTGCTCTTGTGACAG. IL-1β - Forward: CTTGGGCCTCAAAGGAAAGAA; Reverse: AAGACAAACCGTTTTTCCATCTTC; Probe: AGCTGGAGAGTGTGGAT. IL-6 - Forward: TATGAAGTTCCTCTCTGCAAGAGA; Reverse: TAGGGAAGGCCGTGGTT; Probe: CCAGCATCAGTCCCAAGAAGGCAACT. IL-23p19 - Forward: TCTCTGCATGCTAGCCTGGAA; Reverse: ACAACCATCTTCACACTGGATACG; Probe: CGGGACATATGAATCTA. IL-12p35 - Forward: AAGACATCACACGGGACCAAA; Reverse: CAGGCAACTCTCGTTCTTGTGTA; Probe: CAGCACATTGAAGACCTGTTTACCACTGGA.
PCR reactions were done on an ABI Prism 7300 Sequence Detection System (Applied Biosystems). Results were expressed relative to 18S rRNA (2ΔCT method) as endogenous control (TaqMan® Ribosomal RNA control Reagents kit; Applied Biosystems). cDNA was diluted 1/500 for 18S rRNA analysis.
Statistical analysis
All experiments were repeated at least three times and data are presented as means ± SEM. Statistical significance was assessed using the two-tailed Mann–Whitney U-test (GraphPad Prism 4). P values less than 0.05 were considered significant.
Discussion
We show that three different populations of potential APC can be isolated from the CNS of mice with EAE. These are individual and distinct in terms of their ability to promote CD4+ T cell proliferation as well as to induce pro-inflammatory cytokines. Infiltrating CD11c+ cells and an inflammation-associated subset of CNS-resident CD11c+ microglia show equivalent and potent ability to induce proliferation of antigen-primed CD4+ T cells. However they differ in their expression of Th1- and Th17-inducing cytokines and in their quantitative ability to induce such T cell responses, CD11c+ microglia being noticeably less effective. In contrast to these, CNS-resident CD11c− microglia express low levels of MHC II and co-stimulatory molecules and are poor inducers of T cell proliferation. Despite higher expression of Th1- and Th17-inducing cytokines than their CD11c+ counterparts, they do not induce meaningful Th1 or Th17 responses. The distinct cytokine-producing and response-inducing capabilities of these APC subpopulations identify the complexity of the inflammatory milieu in the CNS in diseases such as MS.
The need for parenchymal APC is based on the fundamental immunological principle of reactivation for CD4
+ T cell effector function within the target tissue. A role for DC in directing T cell transit from the perivascular space in postcapillary venules has been proposed [
10,
15]. The possibility that such T cells would exert adequate effector function to induce pathology immediately after crossing the glia limitans cannot be excluded. However, competent microglia are also required for EAE and microglia have been shown to induce final effector CD4
+ T cell response [
9,
16]. Furthermore, reactivation of effector function in T cells that migrate deeper than the juxtavascular zone would either require co-infiltrating or already-resident APC. Such considerations motivated our study. Our findings confirm the importance of co-infiltrating CD11c
+ APC for T cell response in the CNS, but also identify CNS-resident CD11c
+ APC that can mediate a qualitatively similar outcome.
Despite lack of expression of most of the cytokines that are conventionally associated with Th1 and Th17 induction, CD11c
+ microglia could nevertheless induce both IFN-γ and IL-17A
in vitro, although at low levels. IL-17A induction can be explained by the production of TGF-β and IL-1β by CD11c
+ microglia. Expression of TGF-β was equivalent to that in infiltrating CD11c
+ populations and levels of IL-1β were likely sufficient to override the induction of regulatory T cells [
17]. The induction of a functional Th17 response by IL-1β + TGF-β producing CD11c
+ microglia that we observed may then reflect the supplementing contribution of IL-6 or IL-23 produced either by other APC contaminating the T cell population, or by
in vitro induction in microglial APC. IL-12-independent induction of Th1 responses has been described that depends on Type I IFN and IL-18 production by APC [
18]. Microglia are known producers of both these cytokines [
19‐
21], so this is a likely explanation for the Th1 responses we observed
in vitro. Taken together, the observation is that both infiltrating APC and CNS-resident CD11c
+ microglia can induce Th1 and Th17 responses, but possibly by different routes. How these different routes influence the outcome of CNS inflammation will require increased knowledge of the effect of these induction pathways on the effector CD4
+ T cell response.
Thus, CD11c+ microglia potently induce T cell proliferation but are weak inducers of Th1 and Th17 differentiation, due to lack of expression of necessary cytokines. At the same time, CD11c− microglia are poor inducers of T cell expansion but do produce Th1- and Th17-inducing cytokines. Since both subsets of microglia co-exist during neuroinflammation it is likely that they synergise to drive T cell proliferation and Th1 and Th17 differentiation, and so together may function as equivalently effective CNS-resident APC to the infiltrating CD11c+ cells. Emergence of the CD45dimCD11c+ subset primarily overrides a deficit in induction of T cell expansion.
The infiltrating CD11c
+ population contained DC. It is noteworthy that their ability to promote T cell proliferation not only was equivalent to that of CD11c
+ microglia, but that both were markedly less effective than splenic DC-containing populations. Neither DC population was characterised in any more detail and it is possible that this difference reflects varying proportions of other CD11c
+ cells such as macrophages or granulocytes which produced a quantitative bias. Alternatively, the CNS is known for its immune quiescence, including downregulation of MHC on infiltrating cells [
22], and it is possible that the activity of DC that enter the CNS is modulated by the local microenvironment. The relatively high levels of neuronal-derived TGF-β in the CNS have been shown to be responsible for deviation of T cell responses towards a regulatory outcome [
23], as well as on microglia [
24], and there may be analogous effects on DC.
DC-like CD11c
+ microglia with neurogenic potential were induced by glatiramer acetate in a transgenic mouse model for Alzheimer’s disease [
25]. Other descriptions of phenotypically DC-like microglia in CNS have not discriminated on the basis of relative CD45 levels or other markers that would differentiate them from actual DC or other CD11c
+ leukocytes [
26]. Microglia derive from mesodermal progenitors in the yolk sac that colonise the CNS early in foetal development [
27]. They are ontogenically distinct from other mononuclear phagocytic cells, being colony stimulating factor-1-independent [
27] and CCR2
− CX3CR1
+[
14]. The origin of the CD11c
+ subset of microglia is of interest. In this study we verified that the two CD11c
+ populations that emerge in the inflamed CNS in EAE are ontogenically as well as functionally and phenotypically distinct. Our previous data from study of cuprizone demyelination suggested that CD11c
+ microglia arise through proliferative expansion of a small pre-existing pool [
11]. Many studies show an increase of microglia via proliferation during EAE [
28‐
30]. We consider it unlikely that CD11c
+ microglia increase at the cost of the entire microglia population, but that they are part of a general microglial expansion, although perhaps more favoured under certain circumstances.
The CNS-resident CD11c+ microglial subset has by now been described under demyelinating, degenerative and inflammatory conditions and so must be recognised as an important component of innate CNS response.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
AW and ML performed the experiments. AW, ML, OC and TO analyzed the data and wrote the paper. All authors read and approved the final manuscript.