Background
Methods
Animals
Interventions and groups
Histopathological examination
Flow cytometric analysis of Th17 and Th9 cells
Polymerase chain reaction(PCR) of IL-17 and IL-9 mRNA
Molecule | Sequence (5' -3') | Length |
---|---|---|
IL-17 | sense:5'GTCAATGCGGAGGGAAAG 3' | 349 bp |
[GenBank:16171] | antisense:5'CACGAAGCAGTTTGGGAC 3' | |
β-actin | sense: 5'CCAGCCTTCCTTCTTGGGTAT 3' | 102 bp |
[GenBank:11461] | antisense: 5'TTGGCATAGAGGTCTTTACGG 3' | |
IL-9 | PCR1 sense: 5'GCATCAGAGACACCAATTACCT 3' | 140 bp |
[GenBank:16198] | PCR1 antisense: 5' TTAAGGAGGGGAGGTTTTGTA 3' | |
PCR2 sense: 5'AACTGATGATTGTACCACACCGTGC 3' | ||
PCR2 antisense: 5' AGGACGGACACGTGATGTTCTTTAG 3' |