Background
Results
Expression of the 32 ECGs
CT-values and ECG regulation
Gene Symbol | CT Range | CT Min. | CT Max. | Mean CT ± SEM | CV(%) |
---|---|---|---|---|---|
RPL37A
| 1.13 | 17.95 | 19.08 | 18.44 ± 0.29 | 1.6 |
HMBS
| 1.39 | 23.96 | 25.35 | 24.61 ± 0.41 | 1.7 |
CASC3
| 1.22 | 22.79 | 24.01 | 23.45 ± 0.42 | 1.83 |
RPLP0
| 1.36 | 17.22 | 18.58 | 17.83 ± 0.34 | 1.91 |
PSMC4
| 1.56 | 21.04 | 22.6 | 21.90 ± 0.46 | 2.12 |
ABL
| 1.92 | 22.95 | 24.87 | 23.71 ± 0.51 | 2.15 |
UBC
| 1.62 | 19.22 | 20.85 | 19.81 ± 0.43 | 2.19 |
GAPDH
| 1.25 | 16.01 | 17.25 | 16.53 ± 0.37 | 2.27 |
POLR2A
| 2.03 | 23.5 | 25.53 | 24.19 ± 0.56 | 2.35 |
MT-ATP6
| 1.39 | 15.18 | 16.57 | 15.66 ± 0.38 | 2.46 |
PES1
| 1.97 | 21.04 | 23.02 | 22.12 ± 0.55 | 2.49 |
TBP
| 2.19 | 23.88 | 26.08 | 24.47 ± 0.64 | 2.62 |
GUSB
| 1.7 | 22.28 | 23.98 | 23.30 ± 0.63 | 2.71 |
EIF2B1
| 2.53 | 23.593 | 26.12 | 24.11 ± 0.70 | 2.91 |
RPS17
| 1.6 | 17.58 | 19.18 | 18.14 ± 0.53 | 2.92 |
RPL30
| 1.89 | 17.24 | 19.14 | 18.05 ± 0.53 | 2.96 |
POP4
| 1.17 | 22.73 | 23.91 | 23.64 ± 0.72 | 3.05 |
PPIA
| 2.15 | 17.43 | 19.58 | 18.08 ± 0.55 | 3.07 |
PUM1
| 2.88 | 22.15 | 25.03 | 23.10 ± 0.71 | 3.08 |
HPRT1
| 2.73 | 21.18 | 23.91 | 22.08 ± 0.68 | 3.1 |
CDKN1B
| 2.08 | 22.8 | 24.98 | 23.98 ± 0.81 | 3.41 |
PGK1
| 2.25 | 18.17 | 20.42 | 19.35 ± 0.69 | 3.61 |
MRPL19
| 3.09 | 22.73 | 25.82 | 24.01 ± 0.95 | 3.96 |
CDKN1A
| 2.6 | 21.3 | 23.99 | 22.98 ± 0.95 | 4.17 |
ACTB
| 5.65 | 16.1 | 21.75 | 17.80 ± 0.75 | 4.23 |
ELF1
| 3.59 | 21.81 | 25.39 | 22.76 ± 1.00 | 4.4 |
IPO8
| 4.26 | 23.68 | 27.93 | 24.80 ± 1.13 | 4.57 |
GADD45A
| 4.2 | 24.37 | 28.57 | 26.29 ± 1.37 | 4.97 |
YWHAZ
| 3.56 | 22.17 | 25.73 | 23.96 ± 1.26 | 5.27 |
TFRC
| 4.26 | 21.15 | 25.41 | 22.13 ± 1.18 | 5.37 |
B2M
| 3.76 | 17.99 | 21.75 | 19.33 ± 1.21 | 6.29 |
18 S
| 2.95 | 8.79 | 11.95 | 10.78 ± 1.02 | 9.5 |
ECG regulation using heat map
Identification of appropriate ECGs
Ranking of the 32 ECGs
A431 |
M
| A549 |
M
| BxPC3 |
M
|
---|---|---|---|---|---|
PGK1-RPL37A
| 0.02 |
RPLPO-UBC
| 0.12 |
RPL37A-RPLPO
| 0.03 |
PSMC4
| 0.04 |
GAPDH
| 0.13 |
CASC3
| 0.03 |
GUSB
| 0.07 |
MT-ATP6
| 0.16 |
RPL30
| 0.08 |
RPLPO
| 0.08 |
CASC3
| 0.19 |
UBC
| 0.08 |
UBC
| 0.09 |
PES1
| 0.27 |
RPS17
| 0.08 |
TBP
| 0.12 |
RPS17
| 0.31 |
EIF2B1
| 0.10 |
GAPDH
| 0.17 |
ACTB
| 0.32 |
ACTB
| 0.12 |
PPIA
| 0.16 |
TBP
| 0.42 |
POP4
| 0.13 |
ABL
| 0.16 |
PPIA
| 0.39 |
PSMC4
| 0.13 |
HPRT1
| 0.17 |
HMBS
| 0.41 |
PPIA
| 0.13 |
RPL30
| 0.17 |
PSMC4
| 0.46 |
ABL
| 0.14 |
PUM1
| 0.19 |
ABL
| 0.51 |
PGK1
| 0.14 |
ELF1
| 0.20 |
POLR2A
| 0.54 |
TBP
| 0.14 |
POLR2A
| 0.22 |
PUM1
| 0.60 |
GUSB
| 0.15 |
CDKN1A
| 0.23 |
RPL30
| 0.64 |
MRPL19
| 0.17 |
POP4
| 0.28 |
EIF2B1
| 0.65 |
CDKN1A
| 0.17 |
PES1
| 0.32 |
HPRT1
| 0.66 |
HPRT1
| 0.18 |
MT-ATP6
| 0.33 |
CDKN1B
| 0.67 |
PUM1
| 0.20 |
CASC3
| 0.32 |
MRPL19
| 0.65 |
HMBS
| 0.21 |
RPS17
| 0.33 |
RPL37A
| 0.68 |
MT-ATP6
| 0.21 |
HMBS
| 0.34 |
ELF1
| 0.73 |
B2M
| 0.23 |
EIF2B1
| 0.37 |
GUSB
| 0.87 |
GAPDH
| 0.25 |
YWHAZ
| 0.39 |
YWHAZ
| 0.88 |
PES1
| 0.27 |
CDKN1B
| 0.40 |
POP4
| 0.92 |
POLR2A
| 0.27 |
MRPL19
| 0.43 |
PGK1
| 0.97 |
YWHAZ
| 0.32 |
ACTB
| 0.48 |
GADD45A
| 1.04 |
ELF1
| 0.33 |
GADD45A
| 0.51 |
B2M
| 1.01 |
TFRC
| 0.36 |
IPO8
| 0.52 |
TFRC
| 1.22 |
IPO8
| 0.37 |
B2M
| 0.52 |
CDKN1A
| 1.28 |
18S
| 0.41 |
TFRC
| 0.64 |
18S
| 1.27 |
GADD45A
| 0.45 |
18S
| 0.96 |
IPO8
| 1.34 |
CDKN1B
| 0.58 |
Calculation of normalization factor
Validation of the gene-stability measure M
Radiation-specific expression of the ECGs within the cell lines
Identifying the consensus set of ECGs for comparative studies across all cell types
Discussion
Symbol | Name |
---|---|
RPLPO
| Ribosomal protein, large, P0 |
UBC
| Ubiquitin C |
PSMC4
| Protease 26S subunit, ATPase, 4 |
PPIA
| Peptidylprolyl isomerase A |
TBP
| TATA box binding protein |