Introduction
Partial pneumonectomy (PNX), the surgical removal of a lung lobe or lobes, substantially diminishes diffusion capacity by reducing the total number of alveoli and the associated vasculature available for gas exchange. The immediate challenge to a pneumonectomized animal is to maintain adequate gas exchange following resection of lung tissue. Physiological compensation for the loss of lung mass is achieved primarily through two mechanisms: enhancement of diffusion capacity in the remnant lung, and/or generation of new pulmonary gas exchange units.
PNX elicits a number of anatomical changes within the thoracic cavity that augment the diffusion capacity of the remaining lobes. Ligation and surgical removal of one or more lobes directs the entire cardiac output into remaining lung tissue and creates an empty hemithorax that results in a shift of the mediastinum toward the vacated thoracic compartment [
1,
2]. In addition, increased space within the chest releases constraints on lung expansion imposed by the thoracic wall. Increased lung inflation on inspiration can then recruit alveoli that might have been incompletely ventilated prior to PNX. Likewise, increased pulmonary blood flow to the remaining tissue may contribute to tissue distension and enhance parenchymal perfusion. As a consequence of these post-surgical anatomical changes, adequate gas exchange can be re-established after PNX by exploiting the physiological reserves of diffusion capacity in the remaining lung tissue.
A second adaptive response to PNX is compensatory growth of the remaining lobes. In a variety of mammalian species, PNX stimulates rapid compensatory lung growth, restoring normal mass, structure and function. Although post-PNX compensatory lung growth has been documented in rabbits [
3], mice [
4,
5], ferrets [
6,
7] and dogs [
8,
9], the response is best characterized in the rat [
10,
11,
12]. Excision of the single lobe of the left lung in rats removes 35% of the total lung mass. Following left PNX, the right lung mass remains stable for 2 post-operative days; subsequently, the right lung mass increases at an accelerated rate until a value equivalent to the total lung mass in sham pneumonectomy (SHAM)-operated animals is achieved [
11]. In rats of about 300 g body weight (BW), restoration of total lung mass is complete approximately 14 days after left PNX [
11].
The post-PNX compensatory growth response is independent of the lobe or lobes removed [
12]; all remaining lobes grow rapidly in proportion to their initial mass until normal total lung mass is restored [
1,
11]. Increases in lung volume parallel accumulation of tissue. The volume of the residual lobes thus increases until a value similar to that of both lungs in age-matched control animals is achieved [
12]. The issue of whether restoration of lung volume is primarily due to formation of new alveoli [
3,
8] or to expansion of existing air spaces [
10] has been subject to much investigation. Development of more sophisticated morphometric methods has permitted accurate analysis of lung volume and alveolar number. The latest data indicate that new alveoli are indeed formed during the growth response [
13]. Compensatory growth of alveolar septal tissue restores both alveolar and capillary surface areas to values comparable with those in lungs of SHAM-operated controls (Table
1), resulting in complete normalization of gas exchange function in lungs of PNX animals [
9,
14].
Table 1
Morphometric analysis of post-operative day 14 PNX and ADX/PNX rat lungs
| 138 ± 6 | 156 ± 5 |
| 140 ± 5* | 113 ± 5 |
| | |
Epithelial | 151 ± 17 | 201 ± 22* |
Type I | 156 ± 17 | 186 ± 23* |
Type II | 189 ± 16 | 303 ± 54* |
Interstitial | 135 ± 19 | 189 ± 30* |
Cellular | 138 ± 21 | 193 ± 26* |
Non-cellular | 133 ± 19 | 189 ± 37* |
Endothelial | 148 ± 14 | 108 ± 21* |
Alveolar macrophages | 205 ± 25 | 235 ± 7* |
| | |
Epithelial | 126 ± 12 | 137 ± 15 |
Endothelial | 114 ± 9 | 142 ± 17* |
Age and gender can also modulate post-PNX compensatory lung growth. Whereas the general characteristics of the response are similar in both adult and immature animals of the same species [
11], the post-surgical interval necessary to replace the resected tissue is substantially shorter in young animals. Young rodents undergo a vigorous compensatory lung growth response that exceeds normal maturational lung growth, restoring morphological and gas diffusion properties to values observed in both lungs of age-matched controls within the first postoperative week [
8,
14,
15]. Nevertheless, the absolute rate of gain in lung mass is nearly equivalent in young and adult rats [
11]. In as much as left PNX removes less tissue in young animals [
11], restoration of total lung mass is completed more rapidly. A similar difference in the rate of compensatory lung growth has been reported in studies examining the effects of gender on lung growth [
16]. Female rodents, which are smaller than age-matched males, complete post-PNX compensatory lung growth more rapidly [
17].
Following PNX, rapid changes in gene expression have been detected in the remaining lobes prior to increases in DNA content or tissue mass. As early as 30 min after surgery, expression of the immediate early genes c-
fos and
junB is elevated [
18]. Concurrent changes in cAMP content and the activities of several cAMP-dependent enzymes are observed during the early post-PNX interval [
19,
20]. Activity of both calmodulin [
20] and ornithine decarboxylase increases in the remaining lung of mice following PNX [
5]. It is probable that altered expression and activity of these intracellular molecules play a role in the cascade of intracellular signal transduction events that ultimately lead to the compensatory growth response.
Biochemical analysis indicates that elevations in DNA polymerase and thymidine kinase activity precede increases in DNA content in the remnant lobes [
21]. DNA synthesis, as measured by [
3H]thymidine incorporation, is increased within 24 hours after PNX and remains elevated for at least 4 days [
2,
22]. Total protein, RNA and DNA content increase in concert, indicating that post-PNX compensatory lung growth is predominantly due to hyperplasia rather than hypertrophy [
13]. Morphometric analysis confirms significant post-operative increases in capillary endothelial, type I and type II epithelial, and interstitial cell numbers (Table
1). Peak levels of [
3H]thymidine incorporation, measured by audioradiography, are observed in endothelial cells, interstitial fibroblasts and alveolar type II cells within 3 days after surgery [
4]. On post-operative day 6, labeling of the epithelial cell DNA is evident in most alveoli, but is more predominant in type I cells than in type II cells [
4]. This time course is consistent with type II cell to type I cell conversion during the growth process [
4,
23]. Collectively, these observations indicate that increased lung mass following PNX is due to induction of cell proliferation in the residual lung tissue, rather than hypertrophy of the remaining cell populations [
10].
Initiation of post-PNX growth
Several general hypotheses have been advanced to account for events that initiate cellular and molecular changes that lead to compensatory lung growth. Mechanical signals, transient hypoxia associated with thoracotomy, and elevated blood flow have been considered; however, no single event has been proven to account for the growth response.
Increased inflation or elevated blood flow to the remaining lobes may be important initiating events in the post-PNX response [
24]. Space created within the thoracic cavity allows increased inflation of residual tissue as the pneumothorax is reduced. This expansion or 'stretch' of the tissue may signal subsequent tissue growth [
1]. Increased lung inflation results in elevated cAMP content [
19], increased protein kinase A activity [
19], and immediate early gene expression, responses very similar to those observed following partial PNX [
18]. Expansion in fetal lungs promotes both tissue growth and differentiation of type II cells into type I cells [
23]. Similarly, mechanical deformation of lung fibroblasts
in vitro stimulates both cellular proliferation and growth factor production [
25].
Furthermore, availability of space within the thoracic cavity modulates the extent of compensatory lung growth. Filling the vacant hemithorax with inert material, such as sponge or wax, prevents both the shift of the mediastinum toward the contralateral hemithorax and overinflation of the residual lobes. Analysis of remnant lung from PNX animals subjected to intrathoracic plombage reveals that the mass and proliferative status of the residual tissue closely resemble that in SHAM-operated animals [
1,
2]. These observations suggest that reduction of intrathoracic space inhibits compensatory growth. Remarkably, removal of thoracic plombage 3 weeks–9 months after PNX elicits rapid increases in both lung volume and mass that are characteristic of those observed during the immediate post-operative compensatory interval [
6,
26]. Taken together, these studies underscore the essential role of mechanical signals in post-PNX compensatory lung growth.
Thoracotomy equilibrates intrathoracic pressure with that of the atmosphere, causing both lungs to collapse. A mild hypoxemia is induced in the time needed to ligate and remove a single lobe [
27]. Surgical closure of the chest cavity and evacuation of the pneumothorax restores both regular breathing and arterial oxygen content; nevertheless, transient perioperative hypoxemia must be considered as a potential signal for initiation of the compensatory response. Intubation and ventilation of rats during thoracotomy does not change the extent or biochemical nature of the subsequent growth response in spite of significant improvements in perioperative PO
2, PCO
2 and pH [
27]. It is therefore unlikely that transient, surgically induced hypoxia is involved in initiation of compensatory growth.
Post-operative changes in pulmonary blood flow have also been considered as possible signals for compensatory lung growth. Increased perfusion, reflecting constant cardiac output to the residual lung tissue, could cause physical distention of the pulmonary vasculature, resulting in a mechanical signal for lung growth and a concurrent increase in growth factors and/or nutrient availability. The effect of increased blood flow has been measured directly in ferrets using a novel technique [
7]. Arterial vessels supplying the caudal left lobe were banded prior to right PNX. The procedure prevented an increase in post-surgical blood flow to this lobe; subsequent physiological and biochemical analyses revealed that the caudal lobe underwent compensatory lung growth in a manner similar to the left cranial lobe where blood flow remained intact. These data provide strong evidence that elevated blood flow is not critical to initiate the compensatory growth response.
In summary, most recent efforts to elucidate signals responsible for initiating post-PNX lung growth indicate that the major stimulus to growth is mechanical strain in the remaining tissue. Although elevated pulmonary blood flow and transient hypoxia occur following PNX, current data suggest they do not contribute significantly to initiation of the overall response.
Clinical relevance
A number of pathophysiological conditions necessitate lung resection in humans. In current clinical practice, pneumonectomy, lobectomy or wedge resections are frequently performed on patients with primary or metastatic lung tumors; nevertheless, cancer recurrence is a frequent post-surgical complication [
55,
56]. The biological mechanisms that underlie recurrence are poorly understood. It is, however, generally accepted that a microenvironment conducive to wound healing or organ growth would also support growth of cancer cells [
57,
58]. Partial resection of organs that have the capacity to undergo compensatory growth may thus also stimulate outgrowth of additional primary tumors or latent micrometastases. In fact, a number of studies in rodents have shown that partial hepatectomy increases both the number of artificially induced metastases and the growth of locally implanted tumors [
59,
60]. The consequence of post-PNX compensatory lung growth on the metastatic process is currently under investigation (LM Brown, unpublished observations, 2001).
The extent of compensatory lung growth in humans following PNX or lobectomy is incompletely investigated; a number of long-term physiological studies suggest, however, that some degree of compensatory growth may occur, especially in children [
61]. In adults, post-operative increases in lung volume appear to depend on alveolar distention and recruitment rather than on alveolar multiplication [
47]. The consequences of PNX in humans have been studied primarily through analysis of post-operative pulmonary function. Interpretation of data gathered from pulmonary function tests must be qualified with the knowledge that PNX is rarely performed in healthy human subjects. Residual lung disease and patient illness frequently compromise the condition of the remaining lobes [
47] and thus the potential for post-PNX compensatory growth in otherwise healthy adults is poorly defined.
Conclusion
Animal models offer invaluable tools for understanding both physiological processes and mechanisms of human disease. Because post-PNX lung growth occurs in a number of species widely used in biological research, potential applications of the PNX model are numerous. Experimental observations in rats provide a foundation for subsequent studies in other species, including dogs and mice, each of which offers unique advantages. For example, dogs are easily trained to exercise in a controlled environment and may therefore offer an excellent vehicle to define mechanisms by which diffusion capacity is recruited and maintained following partial resection of the lung.
Although most studies of compensatory lung growth have been performed in rats and dogs, mice may prove a more powerful research tool. Commercial availability of genetically altered mice will support investigations of the consequences of gain and/or loss of gene function on the compensatory growth response. Transgenic and knockout technologies, which can be applied most efficiently in mice, will permit the genetic basis of both the physiological and pathophysiological aspects of compensatory organ growth to be elucidated. Cytokines and growth factors essential to liver regeneration following partial hepatectomy have been identified utilizing these types of animal models [
62]. Investigations of compensatory lung growth in transgenic mice may thus offer a unique
in vivo system to elucidate cellular and molecular events that initiate, regulate, and terminate both normal and compensatory growth of the lung.
Finally, our recent studies of pulmonary tumorigenesis in lungs of partially pneumonectomized animals suggest that mice provide a unique model system that may facilitate definition of both the physiological and pathological mechanisms that underlie the stages of lung cancer progression. By modulating gene expression in these animals, the contribution of individual gene products to lung tumorigenesis may be delineated, along with their potential role in human lung cancer.