Background
Methods
Patients and tumor samples
Factor | No. | % |
---|---|---|
Age (years)
| ||
≤50 | 63 | 60 |
>50 | 42 | 40 |
Pathological type
| ||
Invasive ductal carcinomas | 93 | 88.6 |
Others | 12 | 11.4 |
Stage
| ||
I | 6 | 5.7 |
II | 34 | 32.4 |
III | 65 | 61.9 |
Tumor size
| ||
≤2 cm | 19 | 18.1 |
>2 cm | 86 | 81.9 |
Relapse
| ||
Yes | 38 | 36.2 |
No | 67 | 63.8 |
Lymph node metastasis
| ||
0-4 | 45 | 42.9 |
5-8 | 39 | 37.1 |
≥9 | 21 | 20.0 |
Status
| ||
Dead | 67 | 63.8 |
Alive | 38 | 36.2 |
Cell culture, plasmids, lentivirus and siRNAs
Cell proliferation assay
Cell invasion and migration assays
Total RNA isolation and qRT-PCR assay
Western blotting analysis
Tissue microarray preparation
Immunohistochemical analysis
Statistical analysis
Results
The expression level of ETV1 is inverse with COP1 in clinical TNBC samples
The expression status of ETV1 and COP1 is related with clinical factors and prognosis of TNBC patients
Characteristics | ETV1 |
χ
2
| Pvalue | COP1 |
χ
2
| Pvalue | ||
---|---|---|---|---|---|---|---|---|
Negative | Positive | Negative | Positive | |||||
Age (y)
| ||||||||
≤50 | 14 | 49 | 0.545 | 0.460 | 41 | 17 | 0.213 | 0.645 |
>50 | 12 | 30 | 35 | 12 | ||||
Tumor Size
| ||||||||
≤2 cm | 5 | 14 | −0.030 | 0.535▲ | 13 | 6 | 0.182 | 0.443 |
>2 cm | 21 | 65 | 63 | 23 | ||||
Pathological type
| ||||||||
Invasive ductal carcinomas | 21 | 72 | −2.078 | 0.139▲ | 69 | 24 | 1.337 | 0.204 |
Others | 5 | 7 | 7 | 5 | ||||
TNM Stage
| ||||||||
I | 3 | 3 | −6.220 | 0.045▲* | 2 | 4 | −7.466 | 0.024▲* |
II | 12 | 22 | 22 | 12 | ||||
III | 11 | 54 | 52 | 13 | ||||
Lymph node metastasis
| ||||||||
0-4 | 17 | 28 | −16.986 | 0.028▲* | 31 | 22 | −10.405 | 0.006▲* |
5-8 | 6 | 33 | 29 | 5 | ||||
≥9 | 3 | 18 | 16 | 2 | ||||
Relapse
| ||||||||
No | 23 | 44 | 9.094 | 0.002 | 41 | 26 | −11.590 | 0.000▲* |
Yes | 3 | 35 | 35 | 3 |
Regression coefficient | SE | Relative risk (RR) | 95.0% CI for RR |
P
| ||
---|---|---|---|---|---|---|
Lower | Upper | |||||
TNM stage | 0.514 | 0.088 | 1.672 | 1.407 | 1.987 | 0.009* |
Relapse | −0.780 | 0.586 | 0.458 | 0.145 | 1.446 | 0.183 |
Lymph node | 0.434 | 0.406 | 1.544 | 0.696 | 3.425 | 0.285 |
ETV1 | 0.405 | 0.158 | 1.502 | 1.100 | 2.044 | 0.028* |
COP1 | −0.431 | 0.753 | 0.650 | 0.149 | 6.732 | 0.000* |
Age | 0.291 | 0.268 | 1.338 | 0.792 | 2.261 | 0.276 |
Tumor size | 0.449 | 0.610 | 1.566 | 0.474 | 5.175 | 0.462 |
Pathological type | 0.857 | 0.677 | 2.357 | 0.626 | 8.879 | 0.205 |