Background
Methods
Animals
Drugs
Scientific name | Part used | Proportion of ingredients (100 %) |
---|---|---|
Astragalus mongholicus | Root | 12 |
Codonopsis pilosula | Root | 12 |
Rhizoma Atractylodis Macrocephalae | Rhizome | 12 |
Poria cocos | Sclerotium | 12 |
Fructus Aurantii | Fruit | 12 |
Amomum villosum | Fruit | 6.4 |
Ligusticum chuanxiong Hort. | Sclerotium | 9.6 |
Rhizoma corydalis | Rhizoma | 9.6 |
Medicated Leaven | Fermentation products | 12 |
Glycyrrhiza uralensis Fisch. | Root | 2.4 |
Food intake measurement
Sucrose preference test (SPT)
Gastric balloon distensions for electromyographic (EMG) testing [28]
Immunohistochemistry
Real-time PCR
ELISA
Data analysis
Results
XSLJZD increased the food intake of the rats with FD
XSLJZD increased the percentage of sucrose consumption (>75 %) of rats with FD
XSLJZD reduced the hypersensitivity to gastric distention of rats with FD
Expression of relative neuropeptides in the brain and the stomach of each group
Part | Groups | Ghrelin | CCK-8 | VIP |
---|---|---|---|---|
Stomach | Control | 0.583 ± 0.008 | 0.714 ± 0.042 | 0.823 ± 0.025 |
Model | 0.368 ± 0.010** | 0.467 ± 0.057* | 0.486 ± 0.025** | |
XSLJZD | 0.716 ± 0.050*△△ | 0.706 ± 0.090△ | 0.861 ± 0.107△△ | |
Low dose XSLJZD | 0.356 ± 0.044** | 0.498 ± 0.039 | 0.652 ± 0.082 | |
Domperidone | 0.543 ± 0.163△△ | 0.863 ± 0.122△△ | 0.711 ± 0.092△ | |
Hypothalamus | Control | 0.497 ± 0.036 | 0.825 ± 0.081 | 0.561 ± 0.077 |
Model | 0.268 ± 0.010** | 0.372 ± 0.006** | 0.365 ± 0.017* | |
XSLJZD | 0.417 ± 0.017△△ | 0.995 ± 0.088△△ | 0.594 ± 0.105△ | |
Low dose XSLJZD | 0.372 ± 0.011*△ | 0.540 ± 0.050* | 0.391 ± 0.012 | |
Domperidone | 0.441 ± 0.059△△ | 0.824 ± 0.163△△ | 0.449 ± 0.046 |
XSLJZD increased the neuropeptides in the serum of rats with FD
XSLJZD increased the gene expression of the relative neuropeptides in the stomach
Part | Groups | Ghrelin | CCK | VIP |
---|---|---|---|---|
Stomach | Control | 1.536 ± 0.509 | 0.981 ± 0.069 | 1.092 ± 0.244 |
Model | 0.232 ± 0.172* | 0.291 ± 0.121* | 0.111 ± 0.059* | |
XSLJZD | 1.735 ± 0.747 | 1.212 ± 0.320△△ | 1.127 ± 0.336△ | |
Low dose XSLJZD | 0.439 ± 0.074 | 0.428 ± 0.125 | 0.394 ± 0.139 | |
Domperidone | 1.797 ± 0.869 | 0.637 ± 0.095 | 0.555 ± 0.417 | |
hypothalamus | Control | 0.967 ± 0.172 | 1.075 ± 0.268 | 0.871 ± 0.222 |
Model | 4.917 ± 0.239* | 2.923 ± 0.640* | 2.084 ± 0.442* | |
XSLJZD | 2.646 ± 0.797△ | 2.162 ± 0.511* | 0.777 ± 0.295 | |
Low dose XSLJZD | 2.804 ± 0.407△ | 0.916 ± 0.281△ | 1.268 ± 0.285 | |
Domperidone | 1.925 ± 0.448△ | 1.113 ± 0.247△ | 0.617 ± 0.073△ |