Background
Methods
Microbiology
Campylobacter isolation and identification
Genome sequencing, assembly and annotation
Amplified fragment length polymorphism (AFLP)
PCR detection of hcp and gltA genes
Motility assay
Resistance to bile salts
Gentamicin protection assay
Visualisation of capsular polysaccharide (CPS)
Statistical analysis
Results
Prevalence of hcp and identification of T6SS open reading frames in C. coli and C. jejuni isolates
Species | Total number of isolates | Number of hcp positive strains (%) |
---|---|---|
Campylobacter coli
| 57 | 32 (56.1) |
Campylobacter jejuni
| 59 | 17 (28.8) |
TssA | TssB | TssC. |
TssD
| TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Amino acids | 415 | 161 | 484 | 171 | 130 | 573 | 302 | 299 | 838 | 148 | 465 | 257 | 1175 | ||
REFERENCE
| TssA | TssB | TssC |
TssD
| TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | PCR results | Spp. |
RC008 | + | C.colli | |||||||||||||
RC013 | + | C.colli | |||||||||||||
RC023 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 568 (99.1%) | 302 (100%) | 294 (98.3%) | 758 (90.5%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1171 (99.7%) | + | C.colli |
RC026 | + | C.colli | |||||||||||||
RC037 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC043 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC096 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 791 (94.4%) | 147 (99.3%) | 436 (93.8%) | 257 (100%) | 1151 (97.8%) | + | C.colli |
RC105 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RG106 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC116 | 311 (74.9%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC126 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC127 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC148 | 389 (93.7%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 465 (100%) | 254(98.8%) | 1170 (99.6%) | + | C.colli |
RC182 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 432 (92.9%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC264 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 465 (100%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC269 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC281 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC282 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC284 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC285 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC289 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1168(99.4%) | + | C.colli |
RC291 | + | C.colli | |||||||||||||
RC382 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC383 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC386 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC387 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC430 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 459 (98.7%) | 226 (87.9%) | 1170 (99.6%) | + | C.colli |
RC415 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC428 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.colli |
RC459 | 126 (97.0%) | 567 (99.0%) | 146 (48.3%) | 295(98.7%) | 599 (71.5%) | 1170 (99.6%) | + | C.colli | |||||||
12502 | 413 (99.5%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 127 (97.7%) | 569 (99.3%) | 301 (99.7%) | 294 (98.3%) | 825 (98.4%) | 147 (99.3%) | 464 (99.8%) | 254 (98.8%) | 1171 (99.7%) | + | C.jejuni |
RC018 | 344 (71.1%) | 127 (97.7%) | 569 (99.3%) | 302 (100%) | 821 (98.0%) | 332 (28.3%) | + | C.colli | |||||||
RC039 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 169 (98.8%) | 126 (97.0%) | 541 (94.4%) | 302 (100%) | 294 (98.3%) | 821 (98.0%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC104 | 340 (81.9%) | 155 (96.3%) | 477 (99.4%) | 156 (91.2%) | 115 (88.5%) | 567 (99.0%) | 272 (90.1%) | 296 (99.0%) | 782 (93.3%) | 146(98.6%) | 465 (100%) | 255 (99.2%) | 1158 (98.6%) | + | C.jejuni |
RC168 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 526 (91.8%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC169 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC179 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC186 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC185 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC220 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 416 (89.5%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC270 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC280 | 367 (88.4%) | 156 (96.9%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 226 (87.9%) | 1170 (99.6%) | + | C.jejuni |
RC317 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC410 | + | C.jejuni | |||||||||||||
RC427 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 449 (96.6%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC508 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 294 (98.3%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | 1170 (99.6%) | + | C.jejuni |
RC517 | 412 (99.3%) | 160 (99.4%) | 481 (99.4%) | 171 (100%) | 126 (97.0%) | 567 (99.0%) | 302 (100%) | 820 (97.9%) | 147 (99.3%) | 464 (99.8%) | 257 (100%) | + | C.jejuni | ||
RC526 | 422 (87.2%) | 126 (97.0%) | 147 (49.2%) | 493 (58.8%) | + | C.jejuni |
Relative motility of isolates
AFLP analysis of a subset of C. jejuni and C. coli strains
In vitro cell invasion abilities of hcp+ isolates
Capsule polysaccharide profiles of C. coli isolates
Bile salts resistance
Isolate | Control |
Campylobacter coli
|
Campylobacter jejuni
| ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
12502 | RC037 | RC430 | RC008 | RC013 | RC026 | RC039 | RC317 | RC188 | RC185 | RC179 | |
Bile salt resistance | + | – | – | ± | – | – | – | – | – | – | ± |