Background
Results and discussion
Demography of enrolled subjects
Characteristic | All enrolled subjects (n = 2524) |
Diarrheagenic E. coli (n = 117)a
|
Salmonella spp. (n = 109) |
Shigella spp. (n = 37) |
V. parahaemolyticus (n = 10) | Total of all pathogens (n = 269) |
---|---|---|---|---|---|---|
Age (years), n (%) | ||||||
<1 | 1497 (59.3) | 53 (3.5) | 44 (2.9) | 5 (0.3) | 6 (0.4) | 107 (7.1) |
1–2 | 844 (33.4) | 58 (6.9) | 52 (6.2) | 16 (1.9) | 4 (0.5) | 127 15.0) |
3–4 | 131 (5.2) | 5 (3.8) | 11 (8.4) | 9 (6.9) | 0 (0.0) | 25 (19.1) |
=5 | 52 (2.1) | 1 (1.9) | 2 (3.8) | 7 (13.5) | 0 (0.0) | 10 (19.2) |
Mean age ± SD | 1.2 (1.1) | 1.3 (1.0) | 1.5 (1.1) | 2.9 (1.8) | 1.1 (0.7) | 1.6 (1.3) |
P value | – | 0.002 | <0.001 | <0.001 | 0.836 | <0.001 |
Sex, n (%) | ||||||
Male | 1616 (64.0) | 70 (4.3) | 71 (4.4) | 26 (1.6) | 3 (0.2) | 170 (10.5) |
Female | 908 (36.0) | 47 (5.2) | 38 (4.2) | 11 (1.2) | 7 (0.8) | 99 (10.9) |
P value | – | 0.333 | 0.517 | 0.302 | 0.025 | 0.765 |
Seasons, n (%) | ||||||
Spring (Mar–May) | 584 (23.1) | 22 (3.8) | 28 (4.8) | 0 (0.0) | 1 (0.1) | 50 (8.6) |
Summer (Jun–Aug) | 1029 (40.8) | 62 (6.0) | 50 (4.9) | 31 (3.0) | 6 (0.6) | 146 (14.2) |
Autumn (Sep–Nov) | 615 (24.4) | 27 (4.4) | 25 (4.1) | 6 (0.9) | 3 (0.5) | 61 (9.9) |
Winter (Dec–Feb) | 296 (11.7) | 6 (5.1) | 6 (5.1) | 0 (0.0) | 0 (0.0) | 12 (2.0) |
P value | – | 0.017 | 0.229 | <0.001 | 0.397 | <0.001 |
Symptom, n (%) | ||||||
Fever (>38 °C) | 181 (7.2) | 3 (2.6)b
| 21 (19.3)b
| 14 (37.8)b
| 0 (0.0) | 38 (14.1)b
|
Abdominal pain | 112 (4.4) | 4 (3.4) | 8 (7.3) | 7 (18.9)b
| 0 (0.0) | 19 (7.1)b
|
Vomiting | 165 (6.5) | 6 (5.1) | 5 (4.6) | 9 (24.3)b
| 0 (0.0) | 20 (7.4) |
Dehydration | 11 (0.4) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Watery diarrhea | 758 (30.0) | 38 (32.5) | 20 (18.3)b
| 8 (21.6) | 1 (10.0) | 66 (24.5)b
|
Mucus diarrhea | 1724 (68.3) | 76 (65.0) | 87 (79.8)b
| 26 (70.3) | 8 (80.0) | 193 (71.7) |
Bloody diarrhea | 63 (2.5) | 2 (1.7) | 7 (6.4)b
| 3 (8.1)b
| 1(10.0) | 13 (4.8)b
|
Meandiarrhea frequency ± SD | 4.9 (2.4) | 4.3 (2.3) | 5.7 (2.6) | 6.9 (3.6) | 4.1 (1.6) | 5.2 (2.8) |
Antibiotic consumption | 507 (20.1) | 25 (21.4) | 28 (25.7) | 23 (62.2) | 5 (50.0) | 79 (29.4) |
Distribution of pathogenic bacteria by species and serotype
Any simple pathogen | No. of isolates (%) |
---|---|
Diarrheagenic E.coli (n = 118, 5 serogroups) | 118 (43.1) |
EPEC | |
Atypical (eae+ , bfpA−) | 58 (49.2) |
Typical (eae+ , bfpA +) | 9 (7.6) |
EAEC | |
aggR+ | 28 (23.7) |
ETEC | |
est+ | 9 (7.6) |
elt+ | 6 (5.1) |
Both+ | 2 (1.7) |
EIEC (ipaH +) | 5(4.2) |
STEC (eae + , stx1 +)a
| 1 (0.8) |
Salmonella spp. (n = 109, 33 serotypes) | 109 (39.8) |
S. enteritidis
| 35 (32.1) |
S. typhimurium
| 15 (13.8) |
Salmonella 1, 4, [5], 12:i:- | 13 (11.9) |
S. agona
| 10 (9.2) |
S. paratyphi C
| 4 (3.7) |
S. infantis
| 3 (2.8) |
S. braenderup
| 2 (1.8) |
S. tennessee
| 2 (1.8) |
Other serotypesb
| 25 (22.5) |
Shigella spp. (n = 37, 4 serotypes) | 37 (13.5) |
S. sonnei
| 32 (86.5) |
S. flexneri 2a | 2 (5.4) |
S. flexneri 4c | 2 (5.4) |
S. dysenteriae 2 | 1 (2.7) |
V. parahaemolyticus (n = 10, 3 serotypes) | 10 (3.6) |
V. parahaemolyticus O3:K6 | 8 (80) |
V. parahaemolyticus O3:KUT | 1 (10) |
V. parahaemolyticus O1:KUT | 1 (10) |
Total | 274 (100) |
Mixed infections (n = 5) | |
EPEC/Salmonella (S. enteritidis) | 1 |
EPEC/Salmonella (S. infantis)
| 1 |
EAEC/Salmonella (S. typhimurium) | 1 |
EPEC/Shigella (S. sonnei) | 1 |
EPEC/EAEC | 1 |
Prevalence of pathogenic bacteria associated with era, season, age and clinical symptoms
Antimicrobial resistance of Salmonella and Shigella
Antimicrobial | No. (%) resistance of Salmonella species
| No. (%) resistance of Shigella species
| |||||||
---|---|---|---|---|---|---|---|---|---|
S. enteritidis (n = 35) |
S. typhimurium (n = 15) | 1, 4, [5], 12:i:- (n = 13) |
S. agona (n = 10) | Overall (n = 109) |
S. sonnei (n = 32) |
S. flexneri (n = 4) |
S. dysenteriae (n = 1) | Overall (n = 37) | |
AMP | 21 (60.0) | 12 (80.0) | 11 (84.6) | 1 (10.0) | 51 (46.8) | 31 (96.9) | 4 (100.0) | 0 (0.0) | 35 (94.6) |
CHL | 3 (8.6) | 8 (53.3) | 4 (30.8) | 1 (10.0) | 18 (16.5) | 2 (6.3) | 4 (100.0) | 0 (0.0) | 6 (16.2) |
CIP | 1 (2.9) | 6 (40.0) | 3 (23.1) | 0 (0.0) | 10 (9.2) | 0 (0.0) | 1 (25.0) | 0 (0.0) | 1 (2.7) |
CRO | 7 (20.0) | 0 (0.0) | 2 (15.4) | 0 (0.0) | 10 (9.2) | 10 (31.3) | 1 (25.0) | 0 (0.0) | 11 (29.7) |
GEN | 3 (8.6) | 7 (46.7) | 3 (23.1) | 1 (10.0) | 14 (12.8) | 30 (93.8) | 0 (0.0) | 0 (0.0) | 30 (81.1) |
NAL | 34 (97.1) | 9 (60.0) | 5 (38.5) | 1 (10.0) | 61 (56.0) | 32 (100.0) | 4 (100.0) | 0 (0.0) | 36 (97.3) |
STR | 15 (42.9) | 10 (66.7) | 12 (92.3) | 2 (20.0) | 48 (44.0) | 32 (100.0) | 4 (100.0) | 1 (100.0) | 37 (100.0) |
SUL | 19 (54.3) | 12 (80.0) | 9 (69.2) | 2 (20.0) | 60 (55.0) | 32 (100.0) | 1 (25.0) | 1 (100.0) | 34 (91.9) |
TET | 7 (20.0) | 10 (66.7) | 10 (76.9) | 1 (10.0) | 32 (29.4) | 12 (37.5) | 4 (100.0) | 1 (100.0) | 17 (45.9) |
Multidrug-resistance (MDR) | |||||||||
MDR3 | 22 (62.9) | 12 (80.0) | 11 (84.6) | 1 (10.0) | 54 (50.0) | 32 (100) | 4 (100.0) | 1 (100) | 37 (100) |
MDR4 | 17 (48.6) | 12 (80.0) | 9 (69.2) | 1 (10.0) | 45 (41.3) | 31 (96.9) | 4 (100.0) | 0 (0.0) | 35 (94.6) |
MDR5 | 7 (20.0) | 10 (66.7) | 5 (38.5) | 1 (10.0) | 25 (22.9) | 31 (96.9) | 4 (100.0) | 0 (0.0) | 35 (94.6) |
MDR6 | 2 (5.7) | 7 (46.7) | 4 (30.8) | 1 (10.0) | 14 (12.8) | 18 (56.3) | 3 (75.0) | 0 (0.0) | 21 (56.8) |