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European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

01.12.2008 | Article

Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens

verfasst von: N. Sainsus, V. Cattori, C. Lepadatu, R. Hofmann-Lehmann

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 12/2008

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Abstract

The goal of this study was to develop a liquid culture medium for the rapid isolation, cultivation, identification and subsequent antibiotics susceptibility testing of Helicobacter pylori directly from biopsy specimens. Five liquid media were tested: Ham’s F-12, Brucella broth, tryptic soybroth, brain heart infusion broth and Mueller-Hinton broth. After optimisation of the medium, it was applied in order to investigate biopsy samples from 150 patients with gastro-duodenal disorders and compared with traditional culture methods, microscopy and an H. pylori-specific TaqMan real-time polymerase chain reaction (PCR). The most reliable and rapid growth of H. pylori, even at a small inoculum size, was obtained in Ham’s F-12 medium with 5% horse serum. The developed system allowed the primary isolation of H. pylori in clinical samples and provided 87% sensitivity and 100% specificity.

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Titel: Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens
verfasst von: N. Sainsus
V. Cattori
C. Lepadatu
R. Hofmann-Lehmann
Publikationsdatum: 01.12.2008
Verlag: Springer-Verlag
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 12/2008
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-008-0567-6

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