Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Digestive Diseases and Sciences
Erschienen in: Digestive Diseases and Sciences 4/2018

09.02.2018 | Original Article

Long Noncoding RNA GAPLINC Promotes Cells Migration and Invasion in Colorectal Cancer Cell by Regulating miR-34a/c-MET Signal Pathway

verfasst von: Yuqi Luo, Jun Ouyang, Donggen Zhou, Shizhen Zhong, Minjie Wen, Wentao Ou, Haitao Yu, Lin Jia, Yaoxin Huang

Erschienen in: Digestive Diseases and Sciences | Ausgabe 4/2018

Einloggen, um Zugang zu erhalten

Abstract

Background

Gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC) has been detected in colorectal cancer (CRC) cells and reportedly performs many functions related to tumor proliferation and metastasis. Aim The present study aimed to comprehensively explore the biological functions of GAPLINC and their underlying mechanism in CRC cell.

Methods

The human cancer LncRNA PCR array was used to detect the differentially expressed long noncoding RNAs in human CRC samples. Real-time PCR, dual-luciferase assay, RNA pull-down assay, Transwell assay, and western blot analysis were performed to explore the molecular mechanism underlying GAPLINC functions related to migration and invasion of a human CRC cell line (HCT116).

Results

Compared to the non-cancerous tissues, GAPLINC expression was obviously increased in CRC tissues. In HCT116, silencing of GAPLINC weakened cell migration and invasion, while overexpression of GAPLINC significantly promoted cell migration and invasion. Through dual-luciferase, RNA pull-down, and Transwell assays, we verified that miR-34a was the downstream molecule of GAPLINC and that miR-34a negatively regulated the migration and invasion of HCT116 cell. Furthermore, we found that GAPLINC positively regulated the miR-34a target gene c-MET in CRC tissues.

Conclusions

Our findings revealed that GAPLINC was up-regulated in CRC tissues and was involved in the migration and invasion of CRC cells by regulating miR-34a/c-MET signaling pathway.
Anhänge
Nur mit Berechtigung zugänglich
Literatur
1.
Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990;61:759–767.CrossRefPubMed
2.
Vinson KE, George DC, Fender AW, et al. The Notch pathway in colorectal cancer. Int J Cancer. 2016;138:1835–1842.CrossRefPubMed
3.
Yashiro M. Molecular alterations of colorectal cancer with inflammatory bowel disease. Dig Dis Sci. 2015;60:2251–2263.CrossRefPubMed
4.
Karsa LV, Lignini TA, Patnick J, et al. The dimensions of the CRC problem. Best Pract Res Clin Gastroenterol. 2010;24:381–396.CrossRefPubMed
5.
6.
7.
Ponting CP, Oliver PL, Reik W. Evolution and functions of long noncoding RNAs. Cell. 2009;136:629–641.CrossRefPubMed
8.
9.
10.
Wang Y, Zhang L, Zheng X, et al. Long non-coding RNA LINC00161 sensitises osteosarcoma cells to cisplatin-induced apoptosis by regulating the miR-645-IFIT2 axis. Cancer Lett. 2016;382:137–146.CrossRefPubMed
11.
Wei X, Wang C, Ma C, et al. Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1alpha and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia. Cancer Cell Int. 2016;16:73.CrossRefPubMedPubMedCentral
12.
Xie CH, Cao YM, Huang Y, et al. Long non-coding RNA TUG1 contributes to tumorigenesis of humanosteosarcoma by sponging miR-9-5p and regulating POU2F1 expression. Bio Med Res Int. 2016;37:15031–15041.
13.
Zheng H, Min J. Role of long noncoding RNA HOTAIR in the growth and apoptosis of osteosarcoma cell MG-63. Bio Med Res Int. 2016;2016:5757641.
14.
Liu H, Wang G, Yang L, et al. Knockdown of long non-coding RNA UCA1 increases the tamoxifen sensitivity of breast cancer cells through inhibition of Wnt/beta-catenin pathway. PLoS One. 2016;11:e0168406.CrossRefPubMedPubMedCentral
15.
Ouimet M, Drouin S, Lajoie M, et al. A childhood acute lymphoblastic leukemia- specific lncRNA implicated in prednisolone resistance, cell proliferation, and migration. Oncotarget. 2017;8:7477–7488.CrossRefPubMed
16.
Ren K, Li Z, Li Y, et al. Long non-coding RNA taurine up regulated gene 1 promotes cell proliferation and invasion in gastric cancervia negatively modulating miRNA-145-5p. Oncol Res. 2017;25:789–798.CrossRefPubMed
17.
Yang ZY, Yang F, Zhang YL, et al. LncRNA-ANCR down-regulation suppresses invasion and migration of colorectal cancer cells by regulating EZH2 expression. Cancer Biomark. 2017;18:95–104.CrossRefPubMed
18.
Hu Y, Wang J, Qian J, et al. Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. Cancer Res. 2014;74:6890–6902.CrossRefPubMed
19.
Liu L, Zhao X, Zou H, et al. Hypoxia promotes gastric cancer malignancy partly through the HIF-1α dependent transcriptional activation of the long non-coding RNA GAPLINC. Front Physiol. 2016;7:420.PubMedPubMedCentral
20.
Yang P, Chen T, Xu Z, et al. Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO. Oncotarget. 2016;7:42183–42194.PubMedPubMedCentral
21.
Wu XL, He XX, Li S, et al. Long non-coding RNA ucoo2kmd.1 regulates CD44- dependent cell growth by competing for miR-211-3p in colorectal cancer. PLoS One. 2016;11:e0151287.CrossRefPubMedPubMedCentral
22.
Fang XY, Pan HF, Leng RX, et al. Long noncoding RNAs: novel insights into gastric cancer. Cancer Lett. 2015;356:357–366.CrossRefPubMed
23.
Schweiger T, Starkl V, Glueck O, et al. Clinical impact of c-MET expression and mutational status in patients with colorectal cancer lung metastases. Eur J Cardiothorac Surg. 2016;49:1103–1111.CrossRefPubMed
24.
Al-Maghrabi J, Emam E, Gomaa W, et al. c-MET immunostaining in colorectal carcinoma is associated with local disease recurrence. BMC Cancer. 2015;15:676.CrossRefPubMedPubMedCentral
25.
Shoji H, Yamada Y, Taniguchi H, et al. Clinical impact of c-MET expression and genetic mutational status in colorectal cancer patients after liver resection. Cancer Sci. 2014;105:1002–1007.CrossRefPubMedPubMedCentral
26.
Huang G, Zhu H, Shi Y, et al. cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/beta-catenin pathway. PloS One. 2015;10:e0131225.CrossRefPubMedPubMedCentral
27.
Zhao JW, Cheng LM. Long non-coding RNA CCAT1/miR-148a axis promotes osteosarcoma proliferation and migration through regulating PIK3IP1. Acta Biochim Biophys Sin. 2017;25:1–10.CrossRef
28.
Lehmann U, Kreipe H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods. 2001;25:409–418.CrossRefPubMed
29.
He ZZ, Wang YJ, Huang GF, et al. The lncRNA UCA1 interacts with miR-182 to modulate glioma proliferation and migration by targeting Iaspp. Arch Biochem Biophys. 2017;623–624:1–8.CrossRefPubMed
30.
Li N, Zhou P, Zheng J, et al. A polymorphism in the lincRNAENST00000515084 exon was found to modulate breast cancer risk via GWAS-based association analyses. PLoS One. 2014;9:e98251.CrossRefPubMedPubMedCentral
31.
Zhang M, Liu Y, Yu B, et al. Over-expression of long non-coding RNA GAPLINC promotes colorectal cancer cell metastasis and poor prognosis. Int J Clin Exp Med. 2016;9:3203–3208.
32.
Yu F, Zheng J, Mao Y, et al. Long non-coding RNA growth arrest-specific transcript5 (GAS5) inhibits liver fibrogenesis through a mechanism of competing endogenous RNA. J Biol Chem. 2015;290:28286–28298.CrossRefPubMedPubMedCentral
33.
Yang F, Huo XS, Yuan SX, et al. Repression of the long noncoding RNALET by histone deacetylase 3 contributes to hypoxia-mediated metastasis. Mol Cell. 2013;49:1083–1096.CrossRefPubMed
34.
35.
36.
Ma MZ, Li CX, Zhang Y, et al. Long non-coding RNA HOTAIR, a c-Myc activated driver of malignancy, negatively regulates miRNA-130a in gallbladder cancer. Mol Cancer. 2014;13:156.CrossRefPubMedPubMedCentral
37.
Steele G Jr, Busse P, Huberman MS, et al. A pilot study of sphincter-sparing management of adenocarcinoma of the rectum. Arch Surg. 1991;126:696–701.CrossRefPubMed
38.
Li W, Zheng J, Deng J, et al. Increased levels of the long intergenic non-protein coding RNA POU3F3 promote DNA methylation in esophageal squamous cell carcinoma cells. Gastroenterology. 2014;146:1714–1726.CrossRefPubMed
39.
Ma Y, Yang Y, Wang F, et al. Long non-coding RNA CCAL regulates colorectal cancer progression by activating Wnt/beta-catenin signalling pathway via suppression of activator protein 2alpha. Gut. 2015;0:1–12.
40.
Wang Q, Yang L, Hu X, et al. Up regulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo. Oncotarget. 2016;8:3441–3453.PubMedCentral
41.
Nissan A, Stojadinovic A, Mitrani-Rosenbaum S, et al. Colon cancer associated transcript-1: a novel RNA expressed in malignant and pre-malignant human tissues. Int J Cancer. 2012;130:1598–1606.CrossRefPubMed
42.
Chen J, Zhang K, Song H, et al. Long noncoding RNA CCAT1 acts as an oncogene and promotes chemoresistance in docetaxel-resistant lung adenocarcinoma cells. Oncotarget. 2016;7:62474–62489.PubMedPubMedCentral
43.
Guo X, Hua Y. CCAT1: an oncogenic long noncoding RNA in human cancers. J Cancer Res Clin Oncol. 2017;143:555–562.CrossRefPubMed
44.
Wang ZH, Guo XQ, Zhang QS, et al. Long non-coding RNA CCAT1 promotes glioma cell proliferation via inhibiting microRNA-410. Biochem Biophys Res Commun. 2016;480:715–720.CrossRefPubMed
45.
Zhang E, Han L, Yin D, et al. H3K27 acetylation activated-long non-coding RNA CCAT1 affects cell proliferation and migration by regulating SPRY4 and HOXB13 expression in esophageal squamous cell carcinoma. Nucleic Acids Res. 2016;45:3086–3101.CrossRefPubMedCentral
46.
Chao DT, Korsmeyer SJ. BCL-2 family: regulators of cell death. Annu Rev Immunol. 1998;16:395–419.CrossRefPubMed
47.
De Luca T, Pelosi A, Trisciuoglio D, et al. MiR-211 and MITF modulation by Bcl-2 protein in melanoma cells. Mol Carcinog. 2016;55:2304–2312.CrossRefPubMed
48.
Kim UJ, Lee MN, Kim EJ, et al. MicroRNA miR-199a regulates the MET proto-oncogene and the downstream extracellular signal-regulated kinase 2 (ERK2). J Biol Chem. 2008;283:18158–18166.CrossRefPubMed
49.
50.
Li N, Fu H, Tie Y, et al. MiR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells. Cancer Lett. 2009;275:44–53.CrossRefPubMed
51.
Dang Y, Luo D, Rong M, et al. Underexpression of miR-34a in Hepatocellular Carcinoma and its contribution towards enhancement of proliferating inhibitory effects of agents targeting c-MET. PLoS One. 2013;8:e61054.CrossRefPubMedPubMedCentral
Metadaten
Titel
Long Noncoding RNA GAPLINC Promotes Cells Migration and Invasion in Colorectal Cancer Cell by Regulating miR-34a/c-MET Signal Pathway
verfasst von
Yuqi Luo
Jun Ouyang
Donggen Zhou
Shizhen Zhong
Minjie Wen
Wentao Ou
Haitao Yu
Lin Jia
Yaoxin Huang
Publikationsdatum
09.02.2018
Verlag
Springer US
Erschienen in
Digestive Diseases and Sciences / Ausgabe 4/2018
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-018-4915-9

Weitere Artikel der Ausgabe 4/2018

Digestive Diseases and Sciences 4/2018 Zur Ausgabe

Original Article

Stool PCR for Gastrointestinal Pathogens in Patients With and Without Immune-Mediated Intestinal Diseases

Case Report

IgG4-Related Disease Mimicking Crohn’s Disease: A Case Report and Review of Literature

Editorial

Gastric Acid and Enteric Infections: Souring on the Use of PPIs

Original Article

Identification of Small Proteins and Peptides in the Differentiation of Patients with Intraductal Mucinous Neoplasms of the Pancreas, Chronic Pancreatitis and Pancreatic Adenocarcinoma

In Memoriam

A Tribute to Paul H. Guth, MD (1927–2017)

Original Article

Gastrointestinal Tract Pathology in a BALB/c Niemann–Pick Disease Type C1 Null Mouse Model

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 | Hypertonie | Nachrichten

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 | Parkinson-Krankheit | Nachrichten

Viel Bewegung in der Parkinsonforschung

25.04.2024 | Nierenkarzinom | Nachrichten

Adjuvante Immuntherapie verlängert Leben bei RCC
weitere anzeigen

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise