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09.02.2016 | Original Article

Long-noncoding RNAs in basal cell carcinoma

verfasst von: Michael Sand, Falk G. Bechara, Daniel Sand, Thilo Gambichler, Stephan A. Hahn, Michael Bromba, Eggert Stockfleth, Schapoor Hessam

Erschienen in: Tumor Biology | Ausgabe 8/2016

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Abstract

Long noncoding RNAs (lncRNAs) are fundamental regulators of pre- and post-transcriptional gene regulation. Over 35,000 different lncRNAs have been described with some of them being involved in cancer formation. The present study was initiated to describe differentially expressed lncRNAs in basal cell carcinoma (BCC). Patients with BCC (n = 6) were included in this study. Punch biopsies were harvested from the tumor center and nonlesional epidermal skin (NLES, control, n = 6). Microarray-based lncRNA and mRNA expression profiles were identified through screening for 30,586 lncRNAs and 26,109 protein-coding transcripts (mRNAs). The microarray data were validated by RT-PCR in a second set of BCC versus control samples. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of mRNAs were performed to assess biologically relevant pathways. A total of 1851 lncRNAs were identified as being significantly up-regulated, whereas 2165 lncRNAs were identified as being significantly down-regulated compared to nonlesional skin (p < 0.05). Oncogenic and/or epidermis-specific lncRNAs, such as CASC15 or ANRIL, were among the differentially expressed sequences. GO analysis showed that the highest enriched GO targeted by up-regulated transcripts was “extracellular matrix.” KEGG pathway analysis showed the highest enrichment scores in “Focal adhesion.” BCC showed a significantly altered lncRNA and mRNA expression profile. Dysregulation of previously described lncRNAs may play a role in the molecular pathogenesis of BCC and should be subject of further analysis.

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Titel: Long-noncoding RNAs in basal cell carcinoma
verfasst von: Michael Sand
Falk G. Bechara
Daniel Sand
Thilo Gambichler
Stephan A. Hahn
Michael Bromba
Eggert Stockfleth
Schapoor Hessam
Publikationsdatum: 09.02.2016
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 8/2016
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-016-4927-z

Springer Medizin

Long-noncoding RNAs in basal cell carcinoma

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