Erschienen in:
01.12.2009
Methods for Preparing Fluorescent and Neutral Red-Stained Whole Mounts of Mouse Mammary Glands
verfasst von:
John D. Landua, Adriana P. Visbal, Michael T. Lewis
Erschienen in:
Journal of Mammary Gland Biology and Neoplasia
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Ausgabe 4/2009
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Abstract
Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.